An integrated biomarker response index for the mussel <i>Mytilus edulis</i> based on laboratory exposure to anthracene and field transplantation experiments -- Journal of Oceanology and Limnology,2017,35(5):1165-1178

	Cite this paper:
	YUAN Mengqi, WANG You, ZHOU Bin, JIAN Xiaoyang, DONG Wenlong, TANG Xuexi. An integrated biomarker response index for the mussel Mytilus edulis based on laboratory exposure to anthracene and field transplantation experiments[J]. Journal of Oceanology and Limnology, 2017, 35(5): 1165-1178

An integrated biomarker response index for the mussel Mytilus edulis based on laboratory exposure to anthracene and field transplantation experiments

YUAN Mengqi, WANG You, ZHOU Bin, JIAN Xiaoyang, DONG Wenlong, TANG Xuexi

College of Marine Life Science, Ocean University of China, Qingdao 266003, China

Abstract:

Organic pollution is a serious environmental problem in coastal areas and it is important to establish quantitative methods for monitoring this pollution. This study screened a series of sensitive biomarkers to construct an integrated biomarker response (IBR) index using Mytilus edulis. Mussels were exposed to the polycyclic aromatic hydrocarbon anthracene under controlled laboratory conditions and the activities of components of the glutathione antioxidant system, and the concentrations of oxidative-damage markers, were measured in the gills and digestive glands. Anthracene exposure resulted in increased levels of malondialdehyde (MDA) and superoxide radicals (O₂·), indicating that oxidative damage had occurred. Correspondingly, anthracene exposure induced increased activities of glutathione S-transferase (GST), glutathione peroxidase (GPx) and reduced glutathione (GSH) in digestive glands, and GPx and glutathione reductase (GR) in gills, consistent with stimulation of the antioxidant system. A field experiment was set up, in which mussels from a relatively clean area were transplanted to a contaminated site. One month later, the activities of GST, GPx and GR had increased in several tissues, particularly in the digestive glands. Based on the laboratory experiment, an IBR, which showed a positive relationship with anthracene exposure, was constructed. The IBR is suggested to be a potentially useful tool for assessing anthracene pollution.

Key words: zooplankton|abundance|biomass|diversity index|Zhangzi Island waters

Received: 2016-03-29 Revised: 2016-05-11

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Articles by YUAN Mengqi

Articles by WANG You

Articles by ZHOU Bin

Articles by JIAN Xiaoyang

Articles by DONG Wenlong

Articles by TANG Xuexi

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