Effects of elevated pCO2 on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae) -- Journal of Oceanology and Limnology,2018,36(2):317-328

	Cite this paper:
	HU Shunxin, WANG You, WANG Ying, ZHAO Yan, ZHANG Xinxin, ZHANG Yongsheng, JIANG Ming, TANG Xuexi. Effects of elevated pCO₂ on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae)[J]. Journal of Oceanology and Limnology, 2018, 36(2): 317-328

Effects of elevated pCO₂ on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae)

HU Shunxin¹, WANG You¹, WANG Ying¹, ZHAO Yan¹, ZHANG Xinxin¹, ZHANG Yongsheng^1,2, JIANG Ming¹, TANG Xuexi¹

1 College of Marine Life Science, Ocean University of China, Qingdao 266003, China;
2 Rongcheng Ocean and Fisheries Bureau, Weihai 264300, China

Abstract:

The present study was conducted to determine the effects of elevated pCO₂ on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga Dunaliella salina. To accomplish this, D. salina was incubated in semi-continuous cultures under present-day CO₂ levels (390 μatm, pH_NBS:8.10), predicted year 2100 CO₂ levels (1 000 μatm, pH_NBS:7.78) and predicted year 2300 CO₂ levels (2 000 μatm, pH_NBS:7.49). Elevated pCO₂ significantly enhanced photosynthesis (in terms of gross photosynthetic O₂ evolution, effective quantum yield (△F/F'_m), photosynthetic efficiency (α), maximum relative electron transport rate (rETR_max) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity) and dark respiration of D. salina, but had insignificant effects on growth. The photosynthetic O₂ evolution of D. salina was significantly inhibited by the inhibitors acetazolamide (AZ), ethoxyzolamide (EZ) and 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), indicating that D. salina is capable of acquiring HCO₃^- via extracellular carbonic anhydrase and anion-exchange pr 3 oteins. Furthermore, the lower inhibition of the photosynthetic O₂ evolution at high pCO₂ levels by AZ, EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high pCO₂. In conclusion, our results show that photosynthesis, dark respiration and CCMs will be affected by the increased pCO₂/low pH conditions predicted for the future, but that the responses of D. salina to high pCO₂/low pH might be modulated by other environmental factors such as light, nutrients and temperature. Therefore, further studies are needed to determine the interactive effects of pCO₂, temperature, light and nutrients on marine microalgae.

Received: 2016-10-08 Revised:

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Articles by HU Shunxin

Articles by WANG You

Articles by WANG Ying

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Articles by JIANG Ming

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