Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China -- Journal of Oceanology and Limnology,2018,36(2):351-361

	Cite this paper:
	MA Yuexin, TAO Wei, LIU Jiao, LIU Changfa, LI Jin, LIU Jichen. Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China[J]. Journal of Oceanology and Limnology, 2018, 36(2): 351-361

Response of ammonia-oxidizing betaproteobacteria to shortterm fertilization in a salt marsh in China

MA Yuexin¹, TAO Wei², LIU Jiao¹, LIU Changfa², LI Jin³, LIU Jichen¹

1 Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;
2 Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China;
3 Institute of Ocean and Fisheries of Panjin, Panjin 124001, China

Abstract:

This study examines the impacts of short-term (6 months) fertilization on the community structure and abundance of ammonia-oxidizing betaproteobacteria (β-AOB) and the potential nitrification rate in sediment colonized by Suaeda heteroptera in a saltmarsh located in Shuangtai estuary, China. The sediment samples were collected from plots treated with different amounts of an N fertilizer (urea supplied at 0.1, 0.2, 0.4, and 0.8 g/kg (nitrogen content in dry sediment)), and with different forms of N fertilizers (urea, (NH₄)₂SO₄, and NH₄NO₃, each supplied at 0.2 g/kg). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August and September of 2013. Untreated plots were included as a control. As revealed in denaturing gradient gel electrophoresis of the 16S rRNA gene, the β-AOB community responded to both the amount and form of N. Real-time quantitative PCR indicated that both abundance and potential nitrification rate of β-AOB increased after N addition, regardless of concentration and form (except NH₄NO₃). These results provide evidence that short-term N application influences the sediment β-AOB community, β-AOB abundance and potential nitrification rate in a saltmarsh ecosystem.

Key words: ammonia-oxidizing betaproteobacteria (β-AOB)|denaturing gradient gel electrophoresis (DGGE)|nitrification|fertilization|saltmarsh

Received: 2016-05-26 Revised:

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