Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (<i>R</i>)-methyl 2-chloropropionate -- Journal of Oceanology and Limnology,2018,36(2):473-482

	Cite this paper:
	GONG Yanhui, MA Sanmei, WANG Yongfei, XU Yongkai, SUN Aijun, ZHANG Yun, HU Yunfeng. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate[J]. Journal of Oceanology and Limnology, 2018, 36(2): 473-482

Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl 2-chloropropionate

GONG Yanhui³, MA Sanmei³, WANG Yongfei³, XU Yongkai⁴, SUN Aijun^1,2, ZHANG Yun^1,2, HU Yunfeng^1,2,5

1 Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
2 Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
3 Department of Biotechnology, Jinan University, Guangzhou 510632, China;
4 Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China;
5 South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China

Abstract:

A novel esterase EstC10 from Bacillus sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC10 was characterized. At present, the reports about the kinetic resolution of racemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate (R)-methyl 2-chloropropionate with high enantiomeric excess (>99%) after the optimization of process parameters such as pH, temperature, organic co-solvents, surfactants, substrate concentration and reaction time. Notably, the optimal substrate concentration (80 mmol/L) of esterase EstC10 was higher than the kinetic resolution of another esterase, Est12-7 (50 mmol/L). The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of (R)-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry.

Key words: biocatalysis|deep-sea microorganisms|novel esterase|kinetic resolution|(R)-methyl 2-chloropropionate

Received: 2016-11-16 Revised:

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Articles by HU Yunfeng

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