Cite this paper:
WU Chunhui, JIANG Peng, ZHAO Jin, FU Huihui. High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. Journal of Oceanology and Limnology, 2018, 36(5): 1806-1811

High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)

WU Chunhui1,2,3, JIANG Peng1,2, ZHAO Jin1,2, FU Huihui1,2
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
Protoplast isolation was relevant for gene manipulation in Ulva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither Fv/Fm value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an effective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast ((31.5±1.9)×106 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
Key words:    protoplast|Ulva prolifera|regeneration|artificial culture   
Received: 2017-03-01   Revised:
Tools
PDF (671 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by WU Chunhui
Articles by JIANG Peng
Articles by ZHAO Jin
Articles by FU Huihui
References:
Bobin-Dubigeon C, Lahaye M, Guillon F, Barry J L, Gallant D J. 1997. Factors limiting the biodegradation of Ulva sp cell-wall polysaccharides. Journal of the Science of Food and Agriculture, 75(3):341-351.
Carl C, de Nys R, Paul N A. 2014. The seeding and cultivation of a tropical species of filamentous Ulva for algal biomass production. PLoS One, 9(6):e98700.
Chen Y C, Shih H C. 2000. Development of protoplasts of Ulva fasciata (Chlorophyta) for algal seed stock. Journal of Phycology, 36(3):608-615.
Cui Y L, Wang J F, Jiang P, Bian S G, Qin S. 2010.Transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) by agitation with glass beads. World Journal of Microbiology & Biotechnology, 26(9):1 653-1 657.
Davey M R, Anthony P, Power J B, Lowe K C. 2005. Plant protoplasts:status and biotechnological perspectives.Biotechnology Advances, 23(2):131-171.
Fisher D D, Gibor A. 1987. Production of protoplasts from the brown alga, Sargassum muticum (Yendo) Fensholt(Phaeophyta). Phycologia, 26(4):488-495.
Hiraoka M, Oka N. 2008. Tank cultivation of Ulva prolifera in deep seawater using a new "germling cluster" method.Journal of Applied Phycology, 20(1):97-102.
Huang X, Weber J C, Hinson T K, Mathieson A C, Minocha S C. 1996. Transient expression of the GUS reporter gene in the protoplasts and partially digested cells of Ulva lactuca L. (Chlorophyta). Botanica Marina, 39(1-6):467-474.
Jiang R, Ingle K N, Golberg A. 2016. Macroalgae (seaweed) for liquid transportation biofuel production:what is next? Algal Research, 14:48-57.
Kerrison P D, Le H N, Twigg G C, Smallman D R, MacPhee R, Houston F A B, Hughes A D. 2016. Decontamination treatments to eliminate problem biota from macroalgal tank cultures of Osmundea pinnatifida, Palmaria palmata and Ulva lactuca. Journal of Applied Phycology, 28(6):3 423-3 434.
Lahaye M, Robic A. 2007. Structure and functional properties of ulvan, a polysaccharide from green seaweeds.Biomacromolecules, 8(6):1 765-1 774.
Leskinen E, Pamilo P. 1997. Evolution of the ITS sequences of ribosomal DNA in Enteromorpha (Chlorophyceae).Hereditas, 126(1):17-23.
Lin A P, Wang C, Qiao H J, Pan G H, Wang G C, Song L Y, Wang Z Y, Sun S, Zhou B C. 2009. Study on the photosynthetic performances of Enteromorpha prolifera collected from the surface and bottom of the sea of Qingdao sea area. Chinese Science Bulletin, 54(3):399-404.
Millner P A, Callow M E, Evans L V. 1979. Preparation of protoplasts from the green alga Enteromorpha intestinalis(L.) Link. Planta, 147(2):174-177.
Reddy C R K, Dipakkore S, Kumar G R, Jha B, Cheney D P, Fujita Y. 2006. An improved enzyme preparation for rapid mass production of protoplasts as seed stock for aquaculture of macrophytic marine green algae.Aquaculture, 260(1-4):290-297.
Reddy C R K, Fujita Y. 1991. Regeneration of plantlets from Enteromorpha (Ulvales, Chlorophyta) protoplasts in axenic culture. Journal of Applied Phycology, 3(3):265-275.
Reddy C R K, Gupta M K, Mantri V A, Jha B. 2008. Seaweed protoplasts:status, biotechnological perspectives and needs. Journal of Applied Phycology, 20(5):619-632.
Saga N. 1984. Isolation of protoplasts from edible seaweeds. The Botanical Magazine, Tokyo, 97(3):423-427.
Uppalapati S R, Fujita Y. 2002. A simple method for mass isolation of protoplasts from species of Monostroma, Enteromorpha and Ulva (Chlorophyta, Ulvales). Journal of Applied Phycology, 14(3):165-168.
Von Stosch H A. 1964. Wirkungen von Jod und Arsenit auf Meersalgen in Kurtur. Proceedings of the 4th International Seaweed Symposium, 4:142-150.
Wang J X, Li A F, Zhou B C. 2011. Studies on developments of microspheres from macroalgal clones and cultivations in airlift photobioreactor. Marine Science, 35(2):17-21. (in Chinese with English abstract)
Wang Y, Wang Y, Zhu L, Zhou B, Tang X X. 2012. Comparative studies on the ecophysiological differences of two green tide macroalgae under controlled laboratory conditions. PLoS One, 7(8):e38245.
Zhang J H, Huo Y Z, Zhang Z L, Yu K F, He Q, Zhang L H, Yang L L, Xu R, He P M. 2013. Variations of morphology and photosynthetic performances of Ulva prolifera during the whole green tide blooming process in the Yellow Sea. Marine Environmental Research, 92:35-42.
Copyright © Haiyang Xuebao