Structure and diversity dynamics of microbial communities at day and night: investigation of meromictic Lake Doroninskoe, Transbaikalia, Russia -- Journal of Oceanology and Limnology,2018,36(6):1978-1992

	Cite this paper:
	Evgeniya MATYUGINA, Natalia BELKOVA, Svetlana BORZENKO, Pavel LUKYANOV, Marsel KABILOV, Olga BATURINA, Alexandra MARTYNOVA-VAN KLEY, Armen NALIAN, Aleksei PTITSYN. Structure and diversity dynamics of microbial communities at day and night: investigation of meromictic Lake Doroninskoe, Transbaikalia, Russia[J]. Journal of Oceanology and Limnology, 2018, 36(6): 1978-1992

Structure and diversity dynamics of microbial communities at day and night: investigation of meromictic Lake Doroninskoe, Transbaikalia, Russia

Evgeniya MATYUGINA¹, Natalia BELKOVA², Svetlana BORZENKO¹, Pavel LUKYANOV¹, Marsel KABILOV³, Olga BATURINA³, Alexandra MARTYNOVA-VAN KLEY⁴, Armen NALIAN⁴, Aleksei PTITSYN¹

1 Institute of Natural Resources, Ecology and Cryology SB RAS, Chita, Russia;
2 Limnological Institute SB RAS, Irkutsk, Russia;
3 Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia;
4 Stephen F. Austin State University, TX, USA

Abstract:

Extreme environmental conditions are key factors in the formation of the structure and diversity of microbial communities. In meromictic ecosystems, extreme conditions and a stable stratification of physical, chemical and biological parameters lead to diversity and heterogeneity of microenvironments. Lake Doroninskoe is located in an extreme geographical area and differs from other known meromictic reservoirs of the world by a low level of illumination in the chemocline and a rare type of alkaline water in sedimentary rocks formed by evaporative concentration. To understand the key factors that shape the composition and structure of the microbial community, the macro- and micro-variations in space and time are of great importance. We investigated the short-term dynamics of the structure and diversity of microbial communities of the meromictic soda lake, Lake Doroninskoe, at day and night using highthroughput sequencing and bioinformatics. Metagenomic analysis of 16S rRNA gene amplicons showed that the microbial communities had a high taxonomic diversity both at day and night. Sixteen bacterial and three archaeal phyla were identified. Proteobacteria were dominant and comprised 75% during the day, increasing to 90% at night. Metabolically stable denitrifying bacteria that were able to use a variety of alternative electron acceptors and electron donors were prevalent in Lake Doroninskoe. They belonged to the families Enterobacteriaceae (class Gammaproteobacteria) and Alcaligenaceae (class Betaproteobacteria). Statistically significant differences between day and night microbial communities were found. During the day, the microbial community was the most diverse. We discuss the peculiarities of the underexplored shortterm dynamics of the structure and diversity of the microbial communities of the meromictic soda Lake Doroninskoe, and propose topics for prospective studies.

Key words: bacterial diversity|day-night dynamics|meromixis|Lake Doroninskoe|high-throughput amplicon sequencing

Received: 2018-01-27 Revised:

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Articles by Evgeniya MATYUGINA

Articles by Natalia BELKOVA

Articles by Svetlana BORZENKO

Articles by Pavel LUKYANOV

Articles by Marsel KABILOV

Articles by Olga BATURINA

Articles by Alexandra MARTYNOVA-VAN KLEY

Articles by Armen NALIAN

Articles by Aleksei PTITSYN

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