Cite this paper:
LI Wanjuan, XUE Shenglun, PANG Min, YUE Zonghao, YANG Dazuo, ZHOU Yibing, ZHAO Huan. The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis[J]. Journal of Oceanology and Limnology, 2018, 36(6): 2297-2307

The expression characteristics of vitellogenin (VTG) in response to B(a)p exposure in polychaete Perinereis aibuhitensis

LI Wanjuan1, XUE Shenglun1, PANG Min2, YUE Zonghao1, YANG Dazuo1, ZHOU Yibing1, ZHAO Huan1
1 Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China;
2 Key Laboratory of Marine Ecology and Environmental Science and Engineering, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Abstract:
In order to investigate the endocrine toxicity of B(a)p to marine polychaete Perinereis aibuhitensis, vitellogenin (VTG) cDNA from the P. aibuhitensis was isolated, recombinated and expressed for the first time. The full length P. aibuhitensis vitellogenin gene (PaVTG) was 5 325 bp, and encoded 1 692 amino acids. It contained the vitellogenin_N domain of unknown function (DUF1943), a von Willebrand factor type D domain, as well as a conserved KALGNAG motif. The expression of VTG gene and protein were mainly up-regulated after exposed to B(a)p at transcriptional and translational levels. PaVTG gene expression did not change significantly at day 4. At day 7 PaVTG expression was up-regulated in 0.5 μg/L and 5 μg/L B(a)p group. At day 14 PaVTG was significantly up-regulated in 0.5-10 μg/L B(a)p. The protein expression of PaVTG in 0.5 μg/L and 10 μg/L B(a)p group was up-regulated with time prolonging, but the expression in 5 μg/L and 50 μg/L B(a)p group exhibited first increased and then decreased trend. With the increasing of B(a)p concentration PaVTG mRNA and protein expression both firstly increased then decreased. In contrast to B(a)p exposure, estradiol did not induce PaVTG gene and protein expression, until late times of exposure (14 d). Overall, the results in this study indicate that PaVTG could be used as a potential indicator of the effects environmental estrogenic compounds.
Key words:    Perinereis aibuhitensis|vitellogenin|B(a)p|expression profiles   
Received: 2017-10-31   Revised:
Tools
PDF (722 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by LI Wanjuan
Articles by XUE Shenglun
Articles by PANG Min
Articles by YUE Zonghao
Articles by YANG Dazuo
Articles by ZHOU Yibing
Articles by ZHAO Huan
References:
Auttarat J, Phiriyangkul P, Utarabhand P. 2006. Characterization of vitellin from the ovaries of the banana shrimp Litopenaeus merguiensis. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 143(1):27-36.
Boulangé-Lecomte C, Xuereb B, Trémolet G, Duflot A, Giusti N, Olivier S, Legrand E, Forget-Leray J. 2017.Controversial use of vitellogenin as a biomarker of endocrine disruption in crustaceans:new adverse pieces of evidence in the copepod Eurytemora affinis.Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 201:66-75.
Charles G D, Bartels M J, Zacharewski T R, Gollapudi B B, Freshour N L, Carney E W. 2000. Activity of benzo[a]pyrene and its hydroxylated metabolites in an estrogen receptor-α reporter gene assay. Toxicological Sciences, 55(2):320-326.
Chen X, Zhou Y B, Yang D Z, Zhao H, Wang L L, Yuan X T. 2012. CYP4 mRNA expression in marine polychaete Perinereis aibuhitensis in response to petroleum hydrocarbon and deltamethrin. Marine Pollution Bulletin, 64(9):1 782-1 788.
Da Silva Rocha A J, Gomes V, De Arruda Campos Rocha Passos M J, Hasue F M, Alves Santos T C, Bícego M C, Taniguchi S, Van Ngan P. 2012. Erod activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene (Bap) concentrations.Environmental Toxicology and Pharmacology, 34(3):995-1003.
Fertuck K C, Matthews J B, Zacharewski T R. 2001.Hydroxylated benzo[a]pyrene metabolites are responsible for in vitro estrogen receptor-mediated gene expression induced by benzo[a]pyrene, but do not elicit uterotrophic effects in vivo. Toxicological Sciences, 59(2):231-240.
Gagné F, Blaise C, Salazar M, Salazar S, Hansen P D. 2001.Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 128(2):213-225.
García C F, Heras H. 2012. Vitellogenin and Lipovitellin from the prawn Macrobrachium borellii as hydrocarbon pollution biomarker. Marine Pollution Bulletin, 64(8):1 631-1 636.
García-Alonso J, Hoeger U, Rebscher N. 2006. Regulation of vitellogenesis in Nereis virens (Annelida:Polychaeta):effect of estradiol-17β on eleocytes. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 143(1):55-61.
Hayakawa K, Onoda Y, Tachikawa C, Hosoi S, Yoshita M, Woon Chung S, Kizu R, Toriba A, Kameda T, Tang N. 2007. Estrogenic/antiestrogenic activities of polycyclic aromatic hydrocarbons and their monohydroxylated derivatives by yeast two-hybrid assay. Journal of Health Science, 53(5):562-570.
Huang D J, Chen H C, Wu J P, Wang S Y. 2006. Reproduction obstacles for the female green neon shrimp (Neocaridina denticulata) after exposure to chlordane and lindane.Chemosphere, 64(1):11-16.
Hwang D S, Lee K W, Han J, Park H G, Lee J, Lee Y M, Lee J S. 2010. Molecular characterization and expression of vitellogenin (Vg) genes from the cyclopoid copepod, Paracyclopina nana exposed to heavy metals.Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 151(3):360-368.
Jørgensen A, Giessing A M B, Rasmussen L J, Andersen O. 2008. Biotransformation of polycyclic aromatic hydrocarbons in marine polychaetes. Marine Environmental Research, 65(2):171-186.
Jubeaux G, Simon R, Salvador A, Quéau H, Chaumot A, Geffard O. 2012. Vitellogenin-like proteins in the freshwater amphipod Gammarus fossarum (Koch, 1835):functional characterization throughout reproductive process, potential for use as an indicator of oocyte quality and endocrine disruption biomarker in males. Aquatic Toxicology, 112-113:72-82.
Kanaly R A, Harayama S. 2000. Biodegradation of highmolecular-weight polycyclic aromatic hydrocarbons by bacteria. Journal of Bacteriology, 182(8):2 059-2 067.
Laffon B, Rábade T, Pásaro E, Méndez J. 2006. Monitoring of the impact of Prestige oil spill on Mytilus galloprovincialis from Galician coast. Environment International, 32(3):342-348.
Lewis C, Watson G J. 2012. Expanding the ecotoxicological toolbox:the inclusion of polychaete reproductive endpoints. Marine Environmental Research, 75:10-22.
Liu P P, Miao J J, Song Y, Pan L Q, Yin P F. 2017. Effects of 2,2',4,4'-tetrabromodipheny ether (bde-47) on gonadogenesis of the manila clam Ruditapes philippinarum. Aquatic Toxicology, 193:178-186.
Marin M G, Matozzo V. 2004. Vitellogenin induction as a biomarker of exposure to estrogenic compounds in aquatic environments. Marine Pollution Bulletin, 48(9-10):835-839.
Matozzo V, Gagné F, Marin M G, Ricciardi F, Blaise C. 2008.Vitellogenin as a biomarker of exposure to estrogenic compounds in aquatic invertebrates:a review. Environment International, 34(4):531-545.
Miao J J, Pan L Q, Liu Jing, Zhang L. 2009. Effects of benzo[a]pyrene on DNA damage and histological alterations in gonad of scallop Chlamys farreri. Marine Environmental Research, 67(1):47-52.
Monteiro P R R, Reis-Henriques M A, Coimbra J. 2000.Polycyclic aromatic hydrocarbons inhibit in vitro ovarian steroidogenesis in the flounder (Platichthys flesus L.).Aquatic Toxicology, 48(4):549-559.
Ni J B, Zeng Z, Kong D Z, Hou L, Huang H Q, Ke C H. 2014.Vitellogenin of fujian oyster, Crassostrea angulata:synthesized in the ovary and controlled by estradiol-17β.General and Comparative Endocrinology, 202:35-43.
OECD. 2010. Workshop report on OECD countries activities regarding testing, assessment and management of endocrine disrupters. OECD, Copenhagen, Denmark.
Park K, Kwak I S. 2010. Molecular effects of endocrinedisrupting chemicals on the Chironomus riparius estrogenrelated receptor gene. Chemosphere, 79(9):934-941.
Phiriyangkul P, Puengyam P, Jakobsen I B, Utarabhand P. 2007. Dynamics of vitellogenin mRNA expression during vitellogenesis in the banana shrimp Penaeus(Fenneropenaeus) merguiensis using real-time PCR.Molecular Reproduction & Development, 74(9):1 198-1 207.
Phoonsamran K, Direkbusarakom S, Chotipuntu P, Hirono I, Unajak S, Summpunn P, Wuthisuthimethavee S. 2017.Identification and Expression of Vitellogenin Gene in Polychaetes (Perinereis sp.). Journal of Fisheries and Environment, 41(1):1-11.
Puinean A M, Labadie P, Hill E M, Osada M, Kishida M, Nakao R, Novillo A, Callard I P, Rotchell J M. 2006.Laboratory exposure to 17β-estradiol fails to induce vitellogenin and estrogen receptor gene expression in the marine invertebrate Mytilus edulis. Aquatic Toxicology, 79(4):376-383.
Smolenaars M M W, Madsen O, Rodenburg K W, Van der Horst D J. 2007. Molecular diversity and evolution of the large lipid transfer protein superfamily. Journal of Lipid Research, 48(3):489-502.
Song Y Y, Yuan X T, Zhang S L, Yang D Z, Hao G, Zhou Y B. 2011. Single and joint toxic effects of benzo(a) pyrene and cadmium on development of three-setiger juvenile of polychaete Pernereis aibuhitensis Grube. Marine Environmental Science, 30(3):333-336. (in Chinese with English abstract)
Thompson J R, Banaszak L J. 2002. Lipid-protein interactions in lipovitellin. Biochemistry, 41(30):9 398-9 409.
Tran T K A, MacFarlane G R, Kong R Y C, O'Connor W A, Yu R M K. 2016. Mechanistic insights into induction of vitellogenin gene expression by estrogens in sydney rock oysters, Saccostrea glomerata. Aquatic Toxicology, 174:146-158.
Volz D C, Chandler G T. 2004. An enzyme-linked immunosorbent assay for lipovitellin quantification in copepods:a screening tool for endocrine toxicity.Environmental Toxicology and Chemistry, 23(2):298-305.
Wang J, Wang W, Zhang X N, Tian H, Ru S G. 2015.Development of a lipovitellin-based goldfish (Carassius auratus) vitellogenin ELISA for detection of environmental estrogens. Chemosphere, 132:166-171.
Wen J M, Pan L Q. 2015. Short-term exposure to benzo[a]pyrene disrupts reproductive endocrine status in the swimming crab Portunus trituberculatus. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 174-175:13-20.
Won S J, Novillo A, Custodia N, Rie M T, Fitzgerald K, Osada M, Callard I P. 2005. The freshwater mussel (Elliptio complanata) as a Sentinel Species:vitellogenin and steroid receptors1. Integrative and Comparative Biology, 45(1):72-80.
Wu Q Y, Wang S Q, Chen X P, Li P. 2017. Reproductive toxicity assessment of benzo[a]pyrene in the marine polychaete Perinereis nuntia. Chinese Journal of Oceanology and Limnology, 35(4):867-873.
Zhang H, Pan L Q, Zhang L. 2012. Molecular cloning and characterization of estrogen receptor gene in the Scallop Chlamys farreri:expression profiles in response to endocrine disrupting chemicals. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 156(1):51-57.
Zhang Q R, Zhou Q X, Wang J, Sun S L, Hua T, Ren L P. 2008.Influences of Cu or Cd on the neurotoxicity induced by petroleum hydrocarbons in ragworm Perinereis aibuhitensis. Journal of Environmental Sciences, 20(3):364-371.
Zhao H, Li W J, Zhao X D, Li X, Yang D Z, Ren H W, Zhou Y B. 2017. Cu/Zn superoxide dismutase (SOD) and catalase(CAT) response to crude oil exposure in the polychaete Perinereis aibuhitensis. Environmental Science and Pollution Research, 24(1):616-627.
Zheng H P, Zhang Q, Liu H L, Liu W H, Sun Z W, Li S K, Zhang T. 2012. Cloning and expression of vitellogenin(Vg) gene and its correlations with total carotenoids content and total antioxidant capacity in noble scallop Chlamys nobilis (Bivalve:Pectinidae). Aquaculture, 366-367:46-53.
Zheng S L, Chen B, Wang Z, Qiu X Y, Yu X G, Freestone D, Liu Z H, Huang H, Yu W W, Xu X Z. 2010. Reproductive toxic effects of sublethal cadmium on the marine polychaete Perinereis nuntia. Ecotoxicology and Environmental Safety, 73(6):1 196-1 201.
Copyright © Haiyang Xuebao