|
|
Cite this paper: |
|
|
ZHOU Liqing, WANG Xuemei, WU Biao, SUN Xiujun, ZHAO Qing, ZHANG Gaowei, LIU Zhihong, YANG Aiguo. Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China[J]. Journal of Oceanology and Limnology, 2019, 37(1): 169-175 |
|
|
|
|
|
|
|
Karyological analysis of the sea cicada Blepharipoda liberate Shen from the Rizhao intertidal zone, China |
|
| ZHOU Liqing1,3, WANG Xuemei2, WU Biao1,3, SUN Xiujun1,3, ZHAO Qing1, ZHANG Gaowei1, LIU Zhihong1,3, YANG Aiguo1,3 |
|
1 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 Rizhao Ocean & Fisheries Research Institute, Rizhao 276826, China;
3 Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China |
|
| Abstract: |
| Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B. liberate is crustacean, its external characteristics are not entirely those of shrimps or crabs. The question of whether B. liberate is a shrimp or a crab has been debated in recent years. We studied the karyotype of B. liberate by light microscopy using air-drying and spreading methods. We obtained mitotic chromosomal plates from B. liberate larvae, and from adult B. liberate females subsequent to egg-laying. The results revealed that B. liberate has 53 pairs of chromosomes (i.e., n=53 and 2n=106), a characteristic shared with four species of crab. The karyogram of B. liberate consists of 25 metacentric, 14 submetacentric, 11 subtelocentric and 3 telocentric pairs. We did not find any heteromorphosis sex chromosomes. Tissue from larvae, gills and ovaries can be used for chromosomal investigations, and we found similar lampbrush chromosomes in ovary cells. Comparatively speaking, larvae tissue is more practical, and ovary tissue is more suitable for the preparation of lampbrush chromosomes. B. liberate is more closely related to crabs than to shrimps, based on the numbers of chromosomes. The B. liberate karyotype reported here provides a basis for further comparative cytogenetic studies of species populations. |
|
| Key words:
Blepharipoda liberate|chromosome|karyotype|lampbrush chromosome
|
|
| Received: 2017-11-27 Revised: 2018-02-05 |
|
|
|
|
References:
Ding P E, Song N, Wang X M, Han Z Q, Gao T X. 2016. The morphological characters and genetic diversity of Blepharipoda liberate Shen and Lophomastix japonica.Acta Hydrobiologica Sinica, 40(6):1 208-1 214. (in Chinese with English abstract)
Galkina S, Deryusheva S, Fillon V, Vignal A, Crooijmans R, Groenen M, Rodionov A, Gaginskaya E. 2006. FISH on avian lampbrush chromosomes produces higher resolution gene mapping. Genetica, 128(1-3):241-251, https://doi.org/10.1007/s10709-005-5776-7.
Gall J G. 2012. Are lampbrush chromosomes unique to meiotic cells? Chromosome Research, 20(8):905-909, https://doi.org/10.1007/s10577-012-9329-5.
Gwatkin R B L. 1977. The egg. In:Gwatkin R B L ed.Fertilization Mechanisms in Man and Mammals. Plenum Press, New York, USA. p.5-12.
Hou Z H, Song Q W, Cui B. 2017. Analysis of nutrient composition in Blepharipoda liberate Shen captured in the wild. Modern Food Science and Technology, 33(6):262-270. (in Chinese with English abstract)
Lee T H, Naitoh N, Yamazaki F. 2004. Chromosome studies on the mitten crabs Eriocheir japonica and E.sinensis.Fisheries Science, 70(2):211-214, https://doi.org/10.1111/j.1444-2906.2003.00793.x.
Levan A, Fredga K, Sandberg A A. 1964. Nomenclature for centromeric position on chromosomes. Hereditas, 52(2):201-220, https://doi.org/10.1111/j.1601-5223.1964.tb01953.x.
Liu F M, Li J Z, Liang Q B, Lin S J. 2016. DNA barcoding and molecular evolution of Rizhao Haichan. Journal of Anhui Agriculture Science, 44(3):133-135. (in Chinese with English abstract)
Macgregor H, Klosterman L. 1979. Observations on the cytology of Bipes (Amphisbaenia) with special reference to its lampbrush chromosomes. Chromosoma, 72(1):67-87.
Mathers T C, Hammond R L, Jenner R A, Hänfling B, Atkins J, Gómez A. 2015. Transition in sexual system and sex chromosome evolution in the tadpole shrimp Triops cancriformis. Heredity, 115(1):37-46.
Montezol M, Cassel M, Silva D, Ferreira A, Mehanna M. 2018. Gametogenesis and reproductive dynamics of Rhinella schneideri (Anura:Bufonidae):influence of environmental and anthropogenic factors. Acta Zoologica, 99(1):93-104, https://doi.org/10.1111/azo.12195.
Piprek R P, Kloc M, Kubiak J Z. 2014. Bidder's organ-structure, development and function. The International Journal of Developmental Biology, 58(10-12):819-827, https://doi.org/10.1387/ijdb.140147rp
Schmid M, Olert J, Klett C. 1979. Chromosome banding in Amphibia. Ⅲ. Sex chromosomes in Triturus.Chromosoma, 71(1):29-55.
Wang Q, Kong X Y, Yu S S, Yu Z N. 2005. Advance in study on chromosome of deoapoda. Marine Sciences, 29(6):60-65. (in Chinese with English abstract)
Wang X M. 2015. The morphological characteristics of the Blepharipoda liberate Shen. Progress in Fishery Sciences, 36(1):74-78. (in Chinese with English abstract)
Yoon B S, Park J H, Yoon S C, Yang J H, Sohn M H. 2014.Community structure of fisheries resources caught by dredge in the Uljin marine ranching area, Korea. Korean Journal of Fisheries and Aquatic Sciences, 47(6):935-944.
Zhu D F, Wang C L, Li Z Q. 2005. Karyotype analysis on Portunus trituberculatus. Journal of Fisheries of China, 29(5):649-653. (in Chinese with English abstract)
|
|
|
|