Effects of florfenicol exposure on growth, development and antioxidant capacity of flounder <i>Paralichthys olivaceus</i> larvae at different developmental stages -- Journal of Oceanology and Limnology,2020,38(2):550-559

	Cite this paper:
	GUO Rui, ZHANG Yingqi, ZHANG Xianyu, ZHANG Qian, CHENG Rui, MD MOSTAFIZUR Rahman, LIU Ying. Effects of florfenicol exposure on growth, development and antioxidant capacity of flounder Paralichthys olivaceus larvae at different developmental stages[J]. Journal of Oceanology and Limnology, 2020, 38(2): 550-559

Effects of florfenicol exposure on growth, development and antioxidant capacity of flounder Paralichthys olivaceus larvae at different developmental stages

GUO Rui^1,2, ZHANG Yingqi^1,2, ZHANG Xianyu^1,2, ZHANG Qian^1,2, CHENG Rui¹, MD MOSTAFIZUR Rahman², LIU Ying^1,2

1 College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China;
2 Liaoning Aquacultural Engineering R&D Center, Dalian 116023, China

Abstract:

Antibiotics are widespread in various environmental media, and may pose a potential threat to aquatic ecosystems and non-target aquatic organisms. Florfenicol (FLO) is one of the most commonly used antibiotics in aquaculture, and extensively used to substitute chloramphenicol with its strong sterilization and low adverse effect. In this study, flounder Paralichthys olivaceus, an important economic fish species in seawater was used as an experimental subject. Five exposure concentrations of FLO (including environmentrelated concentrations) were set at 0, 0.01, 0.1, 1, and 10 mg/L. Effects of FLO exposure for 168 h on growth and development, motor behavior, antioxidant enzyme activity, malondialdehyde (MDA) content, and thyroid hormone level of P. olivaceus larvae were studied in pre-larvae (1 dpf) and post-larvae (20 dpf). The results show that the short-term FLO exposure could promote the larvae growth to some degrees, but inhibit them as the exposure time prolonged. For pre-larvae, FLO at 0.01 mg/L could stimulate the motor nerve system and increase the swimming ability, but inhibited it at 1 mg/L. With the increasing dosage of FLO, the superoxide dismutase (SOD) and MDA contents were elevated, reaching the maximum in the 1 mg/L FLO group. The pre-larvae were more sensitive than the post-larvae to FLO in the environment, and the growth and immune resistance could be damaged with long exposure. Post-larvae were more tolerant to external pollutants, FLO at 1 mg/L could promote the motor behavior and reduce SOD and MDA contents. Therefore, FLO can be used as an antibiotic at a proper concentration but as a drug to prevent disease in a long-term way.

Key words: florfenicol|Paralichthys olivaceus larvae|growth and development|motor behavior|antioxidant capacity

Received: 2019-01-24 Revised: 2019-04-24

Tools

PDF (792 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by GUO Rui

Articles by ZHANG Yingqi

Articles by ZHANG Xianyu

Articles by ZHANG Qian

Articles by CHENG Rui

Articles by MD MOSTAFIZUR Rahman

Articles by LIU Ying

References:
Alonso A, Camargo J A. 2009. Effects of pulse duration and post-exposure period on the nitrite toxicity to a freshwater amphipod. Ecotoxicology and Environmental Safety, 72(7):2 005-2 008, https://doi.org/10.1016/j.ecoenv.2009.06.008.
Biošić M, Mitrevski M, Babić S. 2017. Environmental behavior of sulfadiazine, sulfamethazine, and their metabolites. Environmental Science and Pollution Research, 24(10):9 802-9 812, https://doi.org/10.1007/s11356-017-8639-8.
Botelho R G, Christofoletti C A, Correia J E, Ansoar Y, Olinda R A, Tornisielo V L. 2015. Genotoxic responses of juvenile tilapia (Oreochromis niloticus) exposed to florfenicol and oxytetracycline. Chemosphere, 132:206-212, https://doi.org/10.1016/j.chemosphere.2015.02.053.
Brewster B. 1987. Eye migration and cranial development during flatfish metamorphosis:a reappraisal (Teleostei:Pleuronectiformes). Journal of Fish Biology, 31(6):805-833, https://doi.org/10.1111/j.1095-8649.1987.tb05281.x.
Caipang C M A, Lazado C C, Brinchmann M F, Berg I, Kiron V. 2009. In vivo modulation of immune response and antioxidant defense in Atlantic cod, Gadus morhua following oral administration of oxolinic acid and florfenicol. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 150(4):459-464, https://doi.org/10.1016/j.cbpc.2009.07.001.
Chen H G, Sun L W, Zhang B L et al. 2016. Effects of shortterm exposure to hexabromocyclododecane (HBCD) on T3 and T4 of liver and brain in juvenile crimson snapper(Lutjanus erythopterus). Journal of Agro-Environment Science, 35(7):1 257-1 263, https://doi.org/10.11654/jaes.2016.07.005. (in Chinese with English abstract)
De Jesus E G, Hirano T, Inui Y. 1991. Changes in cortisol and thyroid hormone concentrations during early development and metamorphosis in the Japanese flounder, Paralichthys olivaceus. General and Comparative Endocrinology, 82(3):369-376, https://doi.org/10.1016/0016-6480(91)90312-t.
Dorman D C. 2000. An integrative approach to neurotoxicology.Toxicologic Pathology, 28(1):37-42, https://doi.org/10.1177/019262330002800106.
Fent K, Meier W. 1992. Tributyltin-induced effects on early life stages of Minnows phoxinus phoxinus. Archives of Environmental Contamination and Toxicology, 22(4):428-438, https://doi.org/10.1007/BF00212563.
Hopkins W A, Durant S E, Staub B P, Rowe C L, Jackson B P. 2006. Reproduction, embryonic development, and maternal transfer of contaminants in the amphibian Gastrophryne carolinensis. Environmental Health Perspectives, 114(5):661-666, https://doi.org/10.1289/ehp.8457.
Huang C H, Huang S L. 2004. Effect of dietary vitamin E on growth, tissue lipid peroxidation, and liver glutathione level of juvenile hybrid tilapia, Oreochromis niloticus×O.aureus, fed oxidized oil. Aquaculture, 237(1-4):381-389, https://doi.org/10.1016/j.aquaculture.2004.04.002.
Khan M F, Wang G D. 2018. Environmental agents, oxidative stress and autoimmunity. Current Opinion in Toxicology, 7:22-27, https://doi.org/10.1016/j.cotox.2017.10.012.
Lin T, Yu S L, Chen Y Q, Chen W. 2014. Integrated biomarker responses in zebrafish exposed to sulfonamides.Environmental Toxicology and Pharmacology, 38(2):444-452, https://doi.org/10.1016/j.etap.2014.07.020.
Lundén T, Lilius E M, Bylund G. 2002. Respiratory burst activity of rainbow trout (Oncorhynchus mykiss)phagocytes is modulated by antimicrobial drugs.Aquaculture, 207(3-4):203-212, https://doi.org/10.1016/S0044-8486(01)00760-8.
Mendes R, Cardoso C, Pestana C. 2009. Measurement of malondialdehyde in fish:a comparison study between HPLC methods and the traditional spectrophotometric test. Food Chemistry, 112(4):1 038-1 045, https://doi.org/10.1016/j.foodchem.2008.06.052.
Milan D J, Peterson T A, Ruskin J N, Peterson R T, MacRae C A. 2003. Drugs that induce repolarization abnormalities cause bradycardia in zebrafish. Circulation, 107(10):1 355-1 358, https://doi.org/10.1161/01.CIR.0000061912.88753.87.
Miwa S, Inui Y. 1987. Effects of various doses of thyroxine and triiodothyronine on the metamorphosis of flounder(Paralichthys olivaceus). General and Comparative Endocrinology, 67(3):356-363, https://doi.org/10.1016/0016-6480(87)90190-0.
Politis S N, Servili A, Mazurais D, Zambonino-Infante J L, Miest J J, Tomkiewicz J, Butts I A E. 2018. Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae. General and Comparative Endocrinology, 259:54-65, https://doi.org/10.1016/j.ygcen.2017.11.003.
Reda R M, Ibrahim R E, Ahmed E N G, El-Bouhy Z M. 2013.Effect of oxytetracycline and florfenicol as growth promoters on the health status of cultured Oreochromis niloticus. The Egyptian Journal of Aquatic Research, 39(4):241-248, https://doi.org/10.1016/j.ejar.2013.12.001.
Serrano P H. 2005. Responsible Use of Antibiotics in Aquaculture. Food and Agriculture Organization of the United Nations, Rome. p.1-50.
Shen H Y, Wang L X, Yang J D, Gao J X, Jiao X H, Liu J W. 2014. Effect of streptomycin wastewater on SOD activity and MDA content in muscles of zebra fish. Journal of Hebei University of Science and Technology, 35(3):303-307, https://doi.org/10.7535/hbkd.2014yx03016. (in Chinese with English abstract)
Sørensen L K, Elbæk T H. 2004. Simultaneous determination of trimethoprim, sulfadiazine, florfenicol and oxolinic acid in surface water by liquid chromatography tandem mass spectrometry. Chromatographia, 60(5-6):287-291, https://doi.org/10.1365/s10337-004-0383-9.
Steele S L, Yang X D, Debiais-Thibaud M, Schwerte T, Pelster B, Ekker M, Tiberi M, Perry S F. 2011. In vivo and in vitro assessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio). Journal of Experimental Biology,214:1 445-1 457, https://doi.org/10.1242/jeb.052803.
Yan Z Y, Yang Q L, Jiang W L, Lu J L, Xiang Z R, Guo R X, Chen J Q. 2018. Integrated toxic evaluation of sulfamethazine on zebrafish:including two lifespan stages(embryo-larval and adult) and three exposure periods(exposure, post-exposure and re-exposure). Chemosphere, 195:784-792, https://doi.org/10.1016/j.chemosphere.2017.12.119.
Yang W, Sun H J, Xiang F H, Yang Z, Chen Y F. 2011.Response of juvenile crucian carp (Carassius auratus) to long-term ammonia exposure:feeding, growth, and antioxidant defenses. Journal of Freshwater Ecology, 26(4):563-570, https://doi.org/10.1080/02705060.2011.570944.
Ye S, Hu J T, Zong H M, Na G S, Yao Z W, Wang J Y, Ma D Y. 2008. Determination of florfenicol residues in aquiculture seawater by High Performance liquid chromatographytandem mass spectrometry. Journal of Shenyang Agricultural University, 39(3):368-370, https://doi.org/10.3969/j.issn.1000-1700.2008.03.027. (in Chinese with English abstract)
Yue Z H, Yu M, Zhang X N, Dong Y F, Tian H, Wang W, Ru S G. 2017. Semicarbazide-induced thyroid disruption in Japanese flounder (Paralichthys olivaceus) and its potential mechanisms. Ecotoxicology and Environmental Safety, 140:131-140, https://doi.org/10.1016/j.ecoenv.2017.02.043.
Zhang Q, Cheng J P, Xin Q. 2015a. Effects of tetracycline on developmental toxicity and molecular responses in zebrafish (Danio rerio) embryos. Ecotoxicology, 24(4):707-719, https://doi.org/10.1007/s10646-015-1417-9.
Zhang Q Q, Ying G G, Pan C G, Liu Y S, Zhao J L. 2015b.Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis, multimedia modeling, and linkage to bacterial resistance.Environmental Science & Technology, 49(11):6 772-6 782, https://doi.org/10.1021/acs.est.5b00729.
Zhao H T, Du X X, Zhang K, Liu Y Z, Wang Y J, Liu J X, He Y, Wang X B, Zhang Q Q. 2018. Weighted correlation network analysis (WGCNA) of Japanese flounder(Paralichthys olivaceus) embryo transcriptome provides crucial gene sets for understanding haploid syndrome and rescue by diploidization. Journal of Ocean University of China, 17(6):1 441-1 450, https://doi.org/10.1007/s11802-018-3656-X.