Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone <i>Haliotis discus hannai Ino</i> -- Journal of Oceanology and Limnology,2020,38(6):1936-1944

	Cite this paper:
	LI Xinxin, FENG Xiuni, LUO Kai, MA Shuoli, DENG Junming, ZHANG Wenbing, MAI Kangsen. Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1936-1944

Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino

LI Xinxin¹, FENG Xiuni², LUO Kai¹, MA Shuoli¹, DENG Junming³, ZHANG Wenbing¹, MAI Kangsen¹

1 Key Laboratory of Mariculture(Ministry of Education), Key Laboratory of Aquaculture Nutrition and Feeds(Ministry of Agriculture), Fisheries College, Ocean University of China, Qingdao 266003, China;
2 Jiaozhou Fisheries Technology Promotion Station, Qingdao 266003, China;
3 College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Abstract:

A 240-day feeding trial was conducted to investigate the effects of dietary vitamin C on growth performance, anti-oxidative response, and fatty acid composition of juvenile abalone Haliotis discus hannai Ino (initial body weight:0.93±0.00 g). Three semi-purified experimental diets were formulated containing 0.00, 94.52, and 9 649.58 mg/kg of vitamin C supplied as L-ascorbyl-2-monophosphate. The results show that there was no significant difference in weight gain ratio, daily increment in shell length, and survival rate among the three treatments. Adding dietary vitamin C (9 649.58 mg/kg) significantly increased the activities of superoxide dismutase (SOD), glutathine peroxidase (GPX), glitathione-S-transferase (GST), glutathione reductase (GR), alkaline phosphatase (AKP), and lysozyme in viscera (P<0.05). In muscle, activities of phenoloxidase, catalase, SOD, GST, GR, and AKP were increased in the treatment with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The highest concentrations of ascorbic acid in viscera and muscle were found in the group with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The contents of crude protein and crude lipid in the soft body were significantly increased in the 9 649.58 mg/kg group (P<0.05). Dietary vitamin C supplementation significantly decreased the contents of saturated fatty acids (14:0, 16:0, and 18:0), and increased the composition of 18:2n-6 and 22:6n-3 in the soft body of abalone (P<0.05). Therefore, although there were no significant effects on the growth performance, dietary vitamin C supplementation improved the anti-oxidation and immune responses, increased specific unsaturated fatty acid (i.e., 16:1, 18:1n-7, 18:1n-9, 18:2n-6 and 22:6n-3), and decreased specific saturated fatty acid (i.e., 14:0, 16:0 and 18:0) contents in the soft body of abalone.

Key words: abalone|ascorbic acid|anti-oxidative capability|fatty acid

Received: 2019-08-03 Revised: 2019-09-05

Tools

PDF (538 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by LI Xinxin

Articles by FENG Xiuni

Articles by LUO Kai

Articles by MA Shuoli

Articles by DENG Junming

Articles by ZHANG Wenbing

Articles by MAI Kangsen

References:
Ames B N, Shigenaga M K, Hagen T M. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. Proceedings of the National Academy of Sciences of the United States of America, 90(17):7 915-7 922.
Andersen F, Lygren B, Maage A, Waagbø R. 1998. Interaction between two dietary levels of iron and two forms of ascorbic acid and the effect on growth, antioxidant status and some non-specific immune parameters in Atlantic salmon (Salmo salar) smolts. Aquaculture, 161(1-4):437-451.
AOAC. 1995. Official Methods of Analysis. 16^th edn. AOAC International Publishers, Arlington VA.
Asaikkutti A, Bhavan P S, Vimala K, Karthik M, Cheruparambath P. 2016. Effect of different levels dietary vitamin C on growth performance, muscle composition, antioxidant and enzyme activity of freshwater prawn, Macrobrachium malcolmsonii. Aquaculture Reports, 3:229-236.
Bendich A, Machlin L J, Scandurra O, Burton G W, Wayner D D M. 1986. The antioxidant role of vitamin C. Advances in Free Radical Biology & Medicine, 2(2):419-444.
Berger T M, Polidor M C, Dabbagh A, Evans P J, Halliwell B, Morrow J D, Roberts L J, Frei B. 1997. Antioxidant activity of vitamin C in iron-overloaded human plasma. The Journal of Biological Chemistry, 272(25):15 656-15 660.
Chagas E C, Val A L. 2003. Efeito da vitamina C no ganho de peso e em parâmetros hematológicos de tambaqui. Pesquisa Agropecuária Brasileira, 38(3):397-402.
Chen Y J, Yuan R M, Liu Y J, Yang H J, Liang G Y, Tian L X. 2015. Dietary vitamin C requirement and its effects on tissue antioxidant capacity of juvenile largemouth bass, Micropterus salmoides. Aquaculture, 435:431-436.
Chew B P. 1996. Importance of antioxidant vitamins in immunity and health in animals. Animal Feed Science and Technology, 59(1-3):103-114.
Chien L T, Hwang D F. 2001. Effects of thermal stress and vitamin C on lipid peroxidation and fatty acid composition in the liver of thornfish Terapon jarbua. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 128(1):91-97.
Fishery Bureau, Ministry of Agriculture, People's Republic of China. 2018. China Fishery Statistical Yearbook 2018. China Agriculture Press, Beijing, China. (in Chinese).
Freeman B A, Crapo J D. 1982. Biology of disease:free radicals and tissue injury. Laboratory Investigation, 47(5):412-426.
Gao J, Koshio S, Ishikawa M, Yokoyama S, Mamauag R E P. 2014. Interactive effects of vitamin C and E supplementation on growth performance, fatty acid composition and reduction of oxidative stress in juvenile Japanese flounder Paralichthys olivaceus fed dietary oxidized fish oil. Aquaculture, 422-423:84-90.
Gao J, Koshio S, Ishikawa M, Yokoyama S, Nguyen B T, Mamauag R E. 2013. Effect of dietary oxidized fish oil and vitamin C supplementation on growth performance and reduction of oxidative stress in Red Sea Bream Pagrus major. Aquaculture Nutrition, 19(1):35-44.
Gollas-Galván T, Hernández-López J, Vargas-Albores F. 1997. Effect of Calcium on the prophenoloxidase system activation of the brown shrimp (Penaeus californiensis, Holmes). Comparative Biochemistry and Physiology Part A:Physiology, 117(3):419-425.
Halver J E. 1995. Vitamin requirement study techniques. Journal of Applied Ichthyology, 11(3-4):215-224.
Hu Y, Huang Y, Wen H, Huan Z L, Zhong L, Mao X W, Li J L, Xiao T Y. 2013. Effect of vitamin C on growth, immunity and anti-ammonia-nitrite stress ability in juvenile black carp (Mylopharyngodon piceus). Journal of Fisheries of China, 37(4):565-573. (in Chinese with English abstract)
Hwang D F, Lin T K. 2002. Effect of temperature on dietary vitamin c requirement and lipid in common carp. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 131(1):1-7.
Kosutarak P, Kanazawa A, Teshima S I, Koshio S. 1995. Interactions of L-ascorbyl-2-phosphate Mg and oxidized fish oil on red sea bream juveniles. Fisheries Science, 61(4):696-702.
Lee M H, Shiau S Y. 2002. Dietary vitamin C and its derivatives affect immune responses in grass shrimp, Penaeus monodon. Fish & Shellfish Immunology, 12(2):119-129.
Liang X P, Li Y, Hou Y M, Qiu H, Zhou Q C. 2017. Effect of dietary vitamin C on the growth performance, antioxidant ability and innate immunity of juvenile yellow catfish(Pelteobagrus fulvidraco Richardson). Aquaculture Research, 48(1):149-160.
López N, Cuzon G, Gaxiola G, Taboada G, Valenzuela M, Pascual C, Sánchez A, Rosas C. 2003. Physiological, nutritional, and immunological role of dietary β 1-3 glucan and ascorbic acid 2-monophosphate in Litopenaeus vannamei juveniles. Aquaculture, 224(1-4):223-243.
Lou Q M, Wang Y M, Xue C H. 2013. Lipid and fatty acid composition of two species of abalone, Haliotis discus hannai Ino and Haliotis diversicolor Reeve. Journal of Food Biochemistry, 37(3):296-301.
Mai K S. 1998. Comparative studies on the nutrition of two species of abalone, Haliotis tuberculata L. and Haliotis discus hannai Ino.:VII. Effects of dietary vitamin C on survival, growth and tissue concentration of ascorbic acid. Aquaculture, 161(1-4):383-392.
Metcalfe L D, Schmitz A A, Pelka J R. 1966. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry, 38(3):514-515.
Ming J H, Xie J, Xu P, Ge X P, Liu W B, Ye J Y. 2012. Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Fish & Shellfish Immunology, 32(5):651-661.
Moreau S J M, Doury G, Giordanengo P. 2000. Intraspecific variation in the effects of parasitism by Asobara tabida on phenoloxidase activity of Drosophila melanogaster larvae. Journal of Invertebrate Pathology, 76(2):151-153.
NRC. 2011. Nutrient Requirements of Fish and Shrimp. National Academy Press, Washington, DC, USA. p.207-209.
Ordás M C, Ordás A, Beloso C, Figueras A. 2000. Immune parameters in carpet shell clams naturally infected with Perkinsus atlanticus. Fish & Shellfish Immunology, 10(7):597-609.
Qiao J, Du Z H, Zhang Y L, Du H, Guo L L, Zhong M Q, Cao J S, Wang X Y. 2011. Proteomic identification of the related immune-enhancing proteins in shrimp Litopenaeus vannamei stimulated with vitamin C and Chinese herbs. Fish & Shellfish Immunology, 31(6):736-745.
Ren T J, Koshio S, Uyan O, Komilus C F, Yokoyama S, Ishikawa M, Abdul K. 2008. Effects of dietary vitamin C on blood chemistry and nonspecific immune response of juvenile red sea bream, Pagrus major. Journal of the World Aquaculture Society, 39(6):797-803.
Roosta Z, Hajimoradloo A, Ghorbani R, Hoseinifar S H. 2014. The effects of dietary vitamin C on mucosal immune responses and growth performance in Caspian roach(Rutilus rutilus caspicus) fry. Fish Physiology and Biochemistry, 40(5):1 601-1 607.
Shahkar E, Yun H, Kim D J, Kim S K, Lee B I, Bai S C. 2015. Effects of dietary vitamin C levels on tissue ascorbic acid concentration, hematology, non-specific immune response and gonad histology in broodstock Japanese eel, Anguilla japonica. Aquaculture, 438:115-121.
Shao L Y, Han D, Yang Y X, Jin J Y, Liu H K, Zhu X M, Xie S Q. 2018. Effects of dietary vitamin C on growth, gonad development and antioxidant ability of on-growing gibel carp (Carassius auratus gibelio var. CAS III). Aquaculture Research, 49(3):1 242-1 249.
Wan J J, Ge X P, Liu B, Xie J, Cui S L, Zhou M, Xia S L, Chen R L. 2014. Effect of dietary vitamin C on non-specific immunity and mRNA expression of three heat shock proteins (HSPs) in juvenile Megalobrama amblycephala under pH stress. Aquaculture, 434:325-333.
Wang C C, Huang C H. 2015. Effects of dietary vitamin C on growth, lipid oxidation, and carapace strength of softshelled turtle, Pelodiscus sinensis. Aquaculture, 445:1-4.
Wang L G, Chen D X, Lou B, Zhan W, Chen Y Q, Liu F, Mao G M. 2017. The effects of dietary vitamin C on growth performance, serum enzymes activities and resistance to Vibrio alginolyticus challenge of yellow drum Nibea albiflora. Aquaculture Research, 48(9):4 684-4 695.
Wu C L, Zhang W B, Mai K S, Liang X F, Xu W, Wang J, Ma H M. 2010. Molecular cloning, characterization and mRNA expression of selenium-binding protein in abalone(Haliotis discus hannai Ino):response to dietary selenium, iron and zinc. Fish & Shellfish Immunology, 29(1):117-125.
Wu C L, Zhang W B, Mai K S, Xu W, Zhong X L. 2011. Effects of dietary zinc on gene expression of antioxidant enzymes and heat shock proteins in hepatopancreas of abalone Haliotis discus hannai. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 154(1):1-6.
Wu Y S, Liau S Y, Huang C T, Nan F H. 2016. Beta 1,3/1,6-glucan and vitamin C immunostimulate the nonspecific immune response of white shrimp (Litopenaeus vannamei). Fish & Shellfish Immunology, 57:269-277.
Xu H J, Jiang W D, Feng L, Liu Y, Wu P, Jiang J, Kuang S Y, Tang L, Tang W N, Zhang Y A, Zhou X Q. 2016. Dietary vitamin C deficiency depresses the growth, head kidney and spleen immunity and structural integrity by regulating NF-κB, TOR, Nrf2, apoptosis and MLCK signaling in young grass carp (Ctenopharyngodon idella). Fish & Shellfish Immunology, 52:111-138.
Zhang W B, Mai K S, Xu W, Ma H M. 2003. Metabolic responses to dietary cholecalciferol and phosphorus in abalone Haliotis discus hannai ino. Journal of Experimental Zoology Part A:Comparative Experimental Biology, 299A(2):110-117.
Zhao Y, Zhao J X, Zhang Y, Gao J. 2017. Effects of different dietary vitamin C supplementations on growth performance, mucus immune responses and antioxidant status of loach (Misgurnus anguillicaudatus Cantor) juveniles. Aquaculture Research, 48(8):4 112-4 123.
Zhou Q C, Wang L G, Wang H L, Xie F J, Wang T. 2012. Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum). Fish & Shellfish Immunology, 32(6):969-975.