Molecular markers for identifying a new selected variety of Pacific white shrimp <i>Litopenaeus vannamei</i> -- Journal of Oceanology and Limnology,2015,33(1):1-10

	Cite this paper:
	YU Yang, ZHANG Xiaojun, LIU Jingwen, LI Fuhua, HUANG Hao, LI Yijun, LIU Xiaolin, XIANG Jianhai. Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei[J]. Journal of Oceanology and Limnology, 2015, 33(1): 1-10

Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei

YU Yang^1,2, ZHANG Xiaojun¹, LIU Jingwen^1,2, LI Fuhua¹, HUANG Hao³, LI Yijun³, LIU Xiaolin⁴, XIANG Jianhai¹

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Hainan Nanjiang Marine Biotechnique Company Limited, Sanya 572536, China;
4 Northwest Agriculture and Forestry University, Yangling 712100, China

Abstract:

Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance. However, the identification of new varieties of shrimps from their phenotypic characters is difficult. This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences. The method was employed to identify a new selected variety, Kehai No. 1 (KH-1), from three representative stocks (control group): Zhengda; Tongwei; and a stock collected from Fujian Province, which is now cultured in mainland China. By pooled genotyping of KH-1 and the control group, five microsatellites showing differences between KH-1 and the control group were screened out. Individual genotyping data confirmed the results from pooled genotyping. The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2. By sequencing the mitochondrial control regions of individuals from the KH-1 and control group, four haplotypes were observed in the KH-1 group, whereas 14 haplotypes were obtained in the control group. By combining the microsatellite assignment analysis with mitochondrial control region analysis, the average accuracy of identification of individuals in the KH-1 group and control group reached 89%. The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L. vannamei from other populations cultured in China.

Key words: molecular identification|Litopenaeus vannamei|new variety|mitochondrial control region|microsatellite

Received: 2013-08-20 Revised: 2014-05-04

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