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LI Jiakai, WU Xiangwei, TAN Jing, ZHAO Ruixiang, DENG Lingwei, LIU Xiande. Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock[J]. Journal of Oceanology and Limnology, 2015, 33(4): 919-927

Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

LI Jiakai1, WU Xiangwei2, TAN Jing1, ZHAO Ruixiang1, DENG Lingwei3, LIU Xiande1
1 Key Laboratory of Mariculture in the East China Sea, Ministry of Agriculture of China; Fisheries College, Jimei University, Xiamen 361021, China;
2 Animal Science and Technology College, Yunnan Agricultural University, Kunming 650201, China;
3 Rice Molecular Breeding in Northern Chinese Academy of Sciences Joint Research Center, Harbin 150086, China
P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 cDNA designated PtHSP20 was cloned from P. textile. The full-length cDNA of PtHSP20 is 1 090 bp long and contains a 5' untranslated region (UTR) of 93 bp, a 3' UTR of 475 bp, and an open reading frame (ORF) of 522 bp. The PtHSP20 cDNA encodes 173 amino acid residues and has a molecular mass of 20.22 kDa and an isoelectric point of 6.2. Its predicted amino acid sequence shows that PtHSP20 contains a typical α-crystallin domain (residues 77-171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, PtHSP20 shows moderate homology to other mollusk sHSPs. PtHSP20 mRNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of PtHSP20 mRNA was down-regulated in all of the tissues except the adductor muscle and gonad.
Key words:    Paphia textile|HSP20|high temperature|environmental stress   
Received: 2014-09-15   Revised: 2015-01-18
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