Cite this paper:
Xiaoyue SONG, Jiangning ZENG, Yi ZHOU, Quanzhen CHEN, Hongsheng YANG, Lu SHOU, Yibo LIAO, Wei HUANG, Ping DU, Qiang LIU. Partial function prediction of sulfate-reducing bacterial community from the rhizospheres of two typical coastal wetland plants in China[J]. Journal of Oceanology and Limnology, 2021, 39(1): 185-197

Partial function prediction of sulfate-reducing bacterial community from the rhizospheres of two typical coastal wetland plants in China
Xiaoyue SONG1, Jiangning ZENG1, Yi ZHOU2,3, Quanzhen CHEN1, Hongsheng YANG2,3, Lu SHOU1, Yibo LIAO1, Wei HUANG1, Ping DU1, Qiang LIU1
1 Key Laboratory of Marine Ecosystem Dynamics, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, China;
2 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
3 Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
Abstract:
Sulfate-reducing bacteria (SRB) are ubiquitous anaerobic microorganisms that play significant roles in the global biogeochemical cycle. Coastal wetlands, one of the major habitats of SRB, exhibit high sulfate-reducing activity and thus play significant roles in organic carbon remineralization, benthic geochemical action, and plant-microbe interactions. Recent studies have provided credible evidence that the functional rather than the taxonomic composition of microbes responds more closely to environmental factors. Therefore, in this study, functional gene prediction based on PacBio single molecular real-time sequencing of 16S rDNA was applied to determine the sulfate-reducing and organic substrate-decomposing activities of SRB in the rhizospheres of two typical coastal wetland plants in North and South China:Zostera japonica and Scirpus mariqueter. To this end, some physicochemical characteristics of the sediments as well as the phylogenetic structure, community composition, diversity, and proportions of several functional genes of the SRB in the two plant rhizospheres were analyzed. The Z. japonica meadow had a higher dissimilatory sulfate reduction capability than the S. mariqueter-comprising saltmarsh, owing to its larger proportion of SRB in the microbial community, larger proportions of functional genes involved in dissimilatory sulfate reduction, and the stronger ability of the SRB to degrade organic substrates completely. This study confirmed the feasibility of applying microbial community function prediction in research on the metabolic features of SRB, which will be helpful for gaining new knowledge of the biogeochemical and ecological roles of these bacteria in coastal wetlands.
Key words:    sulfate-reducing bacteria (SRB)|microbial community function prediction|16S rDNA PacBio SMRT sequencing|Zostera japonica|Scirpus mariqueter|rhizosphere   
Received: 2019-07-12   Revised: 2019-09-16
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Articles by Xiaoyue SONG
Articles by Jiangning ZENG
Articles by Yi ZHOU
Articles by Quanzhen CHEN
Articles by Hongsheng YANG
Articles by Lu SHOU
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Articles by Ping DU
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