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Shasha WANG, Yingqiu ZHENG, Muyan CHEN, Kenneth B. STOREY. Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2021, 39(1): 317-328

Ultrastructural variation and key ER chaperones response induced by heat stress in intestinal cells of sea cucumber Apostichopus japonicus

Shasha WANG1, Yingqiu ZHENG1, Muyan CHEN1, Kenneth B. STOREY2
1 Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China;
2 Institute of Biochemistry, Carleton University, Ottawa ON K1S 5B6, Canada
The unfolded protein response (UPR) is an important protective and compensatory strategy used during endoplasmic reticulum stress caused by factors including glucose starvation, low pH, or heat shock. However, there is very little information on the possible role(s) of the UPR under adverse conditions experienced by marine invertebrates. We observed that rough endoplasmic reticulum (ER) was dramatically expanded and numerous autophagosomes were accumulated in the intestinal cells of sea cucumbers, Apostichopus japonicus, under heat stress (4 h at 25℃ compared with 15℃ controls). Moreover, heat stress led to sharp increases in the relative transcript and protein expression levels of two primary ER chaperones:the endoplasmic reticulum resident protein 29-like (ERP29) and protein disulfide-isomerase A6-like (PDIA6). These results suggest a potential adaptive mechanism to deal with heat-induced stress in sea cucumber intestine.
Key words:    Apostichopus japonicus|endoplasmic reticulum (ER)|unfolded protein response (UPR)|endoplasmic reticulum resident protein 29-like (ERP29)|protein disulfide-isomerase A6-like (PDIA6)   
Received: 2019-11-04   Revised: 2020-02-19
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