Cite this paper:
Yanwei FENG, Bo YUAN, Xu JIANG, Xiangquan LIU, Zan LI, Guohua SUN, Xiaohui XU. Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors[J]. Journal of Oceanology and Limnology, 2021, 39(1): 350-361

Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors

Yanwei FENG1, Bo YUAN2,3,4, Xu JIANG5, Xiangquan LIU2, Zan LI1, Guohua SUN1, Xiaohui XU1
1 School of Agriculture, Ludong University, Yantai 264025, China;
2 Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
3 National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
4 Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;
5 Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China
Haliotis discus hannai is an important marine economic species in China. Its egg color was found to be associated with economic traits, which provides a new idea for breeding. However, the molecular mechanism of the egg-color formation has not been reported. Thus, the pigment composition and comparative transcriptome analyses of H. discus hannai with green and gray egg color were conducted using high-performance liquid chromatography (HPLC) and RNA-Seq methods. Results show that individuals with green and gray eggs both possess the fucoxanthin. Lutein existed in gray-egged individuals, but not in green-egged individuals. In transcriptome analysis, 272 310 unigenes were received from 461 162 transcripts with a mean length of 985 bp and N50 of 1 524 bp, respectively. A total of 185 unigenes were identified as differentially expressed genes (DEGs). The DEGs involved in "flavin-containing compound metabolic process", "melanosome", "glutathione metabolism", and "cytochrome b6f complex" were likely related to the formation of the egg color. Our results provide foundational information for the functional analysis of egg-color related genes and are beneficial to the selective breeding of H. discus hannai.
Key words:    Haliotis discus hannai|egg color|high-performance liquid chromatography|transcriptome|differentially expressed genes   
Received: 2019-12-06   Revised: 2020-01-26
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