Cite this paper:
Zhihai ZHONG, Zhengyi LIU, Longchuan ZHUANG, Wanlin SONG, Weizhou CHEN. Effects of temperature on photosynthetic performance and nitrate reductase activity in vivo assay in Gracilariopsis lemaneiformis (Rhodophyta)[J]. Journal of Oceanology and Limnology, 2021, 39(1): 362-371

Effects of temperature on photosynthetic performance and nitrate reductase activity in vivo assay in Gracilariopsis lemaneiformis (Rhodophyta)
Zhihai ZHONG1, Zhengyi LIU1, Longchuan ZHUANG1, Wanlin SONG1, Weizhou CHEN2
1 Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;
2 Shantou University, Shantou 515063, China
Abstract:
Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in China at present. After being acclimated to different growth temperatures (15, 20, 25, and 30℃) for 7 days, the relative growth rate (RGR), nitrate reductase activity, soluble protein content and chlorophyll a fluorescence of G. lemaneiformis were examined. Results show that RGR was markedly affected by temperature especially at 20℃ at which G. lemaneiformis exhibited the highest effective quantum yield of PSⅡ[Y(Ⅱ)] and lightsaturated electron transport rate (ETRmax), but the lowest non-photochemical quenching. Irrespective of growth temperature, the nitrate reductase activity increased with the incubation temperature from 15 to 30℃. In addition, the greatest nitrate reductase activity was found in the thalli grown at 20℃. The value of temperature coefficient Q10 of alga cultured in 15℃ was the greatest among those of other temperatures tested. Results indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity, and the relationship among growth, photosynthesis, and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature.
Key words:    chlorophyll a fluorescence|Gracilariopsis lemaneiformis|growth|nitrate reductase activity   
Received: 2019-11-20   Revised: 2019-12-30
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