Cite this paper:
HAN Tao, WANG Jiteng, HU Shuixin, LI Xinyu, JIANG Yudong, WANG Chunlin. Effects of different dietary lipid sources on growth performance and tissue fatty acid composition of juvenile swimming crab Portunus trituberculatus[J]. Journal of Oceanology and Limnology, 2015, 33(4): 957-965

Effects of different dietary lipid sources on growth performance and tissue fatty acid composition of juvenile swimming crab Portunus trituberculatus

HAN Tao1,2, WANG Jiteng2, HU Shuixin2, LI Xinyu2, JIANG Yudong2, WANG Chunlin1
1 School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
This study was conducted to evaluate the effects of dietary lipid sources on the growth performance and fatty acid composition of the swimming crab, Portunus trituberculatus. Four isonitrogenous and isoenergetic experimental diets were formulated to contain four separate lipid sources, including fish, soybean, rapeseed, and linseed oils (FO, SO, RO, and LO, respectively). With three replicates of 18 crabs each for each diet, crabs (initial body weight, 17.00 ±0.09 g) were fed twice daily for 8 weeks. There were no significant differences among these groups in terms of weight gain, specific growth rate, and hepatosomatic index. However, the RO groups' survival rate was significantly lower than FO groups. The feed conversion and protein efficiency ratios of RO groups were poorer than other groups. The proximate compositions of whole body and hepatopancreas were significantly affected by these dietary treatments. Tissue fatty acid composition mainly reflected dietary fatty acid compositions. Crabs fed FO diets exhibited significantly higher arachidonic, eicosapentaenoic, and docosahexaenoic acid contents in muscle and hepatopancreas compared with VO crabs. Linoleic, oleic, and linolenic acids in muscle and hepatopancreas were the highest in the SO, RO, and LO groups, respectively. The present study suggested that SO and LO could substitute for FO in fishmeal-based diets for swimming crabs, without affecting growth performance and survival.
Key words:    swimming crab|Portunus trituberculatus|lipid source|growth performance|fatty acid composition   
Received: 2014-10-14   Revised: 2015-01-22
PDF ( KB) Free
Print this page
Add to favorites
Email this article to others
Articles by HAN Tao
Articles by WANG Jiteng
Articles by HU Shuixin
Articles by LI Xinyu
Articles by JIANG Yudong
Articles by WANG Chunlin
AOAC. 1995. Official Methods of Analysis Association of Official Analytical Chemists, 16th edn. Association of Official Analytical Chemists, Arlington, VA.
Asdari R, Aliyu-Paiko M, Hashim R, Ramachandran S. 2011. Effects of different dietary lipid sources in the diet for Pangasius hypophthalmus (Sauvage, 1878) juvenile on growth performance, nutrient utilization, body indices and muscle and liver fatty acid composition. Aquaculture Nutrition, 17 (1): 44-53.
Bottino N R, Gennity J, Lilly M L, Simmons E, Finne G. 1980. Seasonal and nutritional effects on the fatty acids of three species of shrimp, Penaeus setiferus, P. aztecus and P. duorarum. Aquaculture, 19 (2): 139-148.
Caballero M J, Izquierdo M S, Kjørsvik E, Montero D, Socorro J, Fernández A J, Rosenlund G. 2003. Morphological aspects of intestinal cells from gilthead seabream (Sparus aurata) fed diets containing different lipid sources. Aquaculture, 225 (1-4): 325-340.
Calder P C. 2001. Polyunsaturated fatty acids, inflammation, and immunity. Lipids, 36 (9): 1 007-1 024.
Castell J D, Kennedy E J, Robinson S M C, Parsons G J, Blair T J, Gonzalez-Duran E. 2004. Effect of dietary lipids on fatty acid composition and metabolism in juvenile green sea urchins (Strongylocentrotus droebachiensis). Aquaculture, 242 (1-4): 417-435.
Catacutan M R. 1991. Growth and fatty acid composition of Penaeus monodon juveniles fed various lipids. The Israeli Journal of Aquaculture-Bamidgeh, 43 (2): 47-56.
Chen G Q, Jiang M M, Chen B, Yang Z F, Lin C. 2006. Emergy analysis of Chinese agriculture. Agriculture, Ecosystems & Environment, 115 (1-4): 161-173.
D'Abramo L R. 1997. Triacylglycerols and fatty acids. In: D'Abramo L R, Conklin D E, Akiyama D M eds. World Aquaculture Society. Baton Rouge, Louisiana. p.71-84.
Dai A Y, Yang S L, Song Y Z. 1986. Marine Crabs in China Sea. Marine Publishing Company, Beijing. Drew M D, Ogunkoya A E, Janz D M, Van Kessel A G. 2007. Dietary influence of replacing fish meal and oil with canola protein concentrate and vegetable oils on growth performance, fatty acid composition and organochlorine residues in rainbow trout (Oncorhynchus mykiss). Aquaculture, 267 (1-4): 260-268.
Folch J, Lee S M, Sloane Stanley G H. 1957. A simple method for the isolation and purification of total lipides from animal tissues. The Journal of Biological Chemistry, 226 (1): 497-509.
Globefish F. 2009. Fish Oil Market Report-January 2009. Food and Agric. Org. of the United Nations, New York.
González-Félix M L, Lawrence A L, Gatlin III D M, Perez- Velazquez M. 2002. Growth, survival and fatty acid composition of juvenile Litopenaeus vannamei fed different oils in the presence and absence of phospholipids. Aquaculture, 205 (3-4): 325-343.
Greene D H, Selivonchick D P. 1990. Effects of dietary vegetable, animal and marine lipids on muscle lipid and hematology of rainbow trout (Oncorhynchus mykiss). Aquaculture, 89 (2): 165-182.
Halver J E, Hardy R W. 2002. Fish Nutrition. 3rd edn. Academic Press.
Han T, Wang J T, Hu S X, Li X Y, Li R H, Mu C K, Wang C L, Yu F P, Shi H L. 2013a. Effect of dietary lipid level on growth and body composition of juvenile swimming crab Portunus trituberculatus. Oceanologia et Limnologia Sinica, 44 (5): 1 276-1 281. (in Chinese with English abstract)
Han T, Wang J T, Hu S X, Li X Y, Li R H, Mu C K, Wang C L. 2013b. Needs of dietary cholesterol for juvenile swimming crab Portunus trituberculatus. Oceanologia et Limnologia Sinica, 44 (6): 1 525-1 529. (in Chinese with English abstract)
Hu Y, Tan B P, Mai K S, Ai Q H, Zhang L, Zheng S X. 2011. Effects of dietary menhaden oil, soybean oil and soybean lecithin oil at different ratios on growth, body composition and blood chemistry of juvenile Litopenaeus vannamei. Aquaculture International, 19 (3): 459-473.
Izquierdo M, Obach A, Arantzamendi L, Montero D, Robaina L, Rosenlund G. 2003. Dietary lipid sources for seabream and seabass: growth performance, tissue composition and flesh quality. Aquaculture Nutrition, 9 (6): 397-407.
Jin M, Zhou Q C, Zhang W, Xie F J, ShenTu J K, Huang X L. 2013. Dietary protein requirements of the juvenile swimming crab, Portunus trituberculatus. Aquaculture, 414-415 : 303-308.
Kanazawa A. 1985. Essential fatty acid and lipid requirement of fish. In : Cowey C B, Mackie A M, Bell J G eds. Nutrition and Feeding in Fish. Academic Press, London. p.281-298.
Kanazawa A, Teshima S. 1977. Biosynthesis of fatty acid from acetate in the prawn, Penaeus japonicus. Mem. Fac. Fish., Kagoshima Univ., 26 : 49-53.
Kanazawa A, Teshima S, Tokiwa S, Kayama M, Hirata M. 1979a. Essential fatty acids in the diet of the prawn - II. Effect of docosahexaenoic acid on growth. Bulletin of the Japanese Society of Scientific Fisheries, 45 : 1 151-1 153.
Kanazawa A, Teshima S, Tokiwa S. 1979b. Biosynthesis of fatty acids from palmitic acid in the prawn, Penaeus japonicus. Mem. Fac. Fish., Kagoshima Univ., 28 : 17-20.
Lee S M. 2001. Review of the lipid and essential fatty acid requirements of rockfish (Sebastes schlegeli). Aquaculture Research, 32 (S1): 8-17.
Li J Y, Guo Z L, Gan X H, Wang D L, Zhang M F, Zhao Y L. 2011. Effect of different dietary lipid sources on growth and gonad maturation of pre-adult female Cherax quadricarinatus (von Martens). Aquaculture Nutrition, 17 (4): e853-e860.
Li X Y, Wang J T, Han T, Hu S X, Jiang Y D, Wang C L. 2014a. Effect of dietary phospholipids levels and sources on growth performance, fatty acid composition of the juvenile swimming crab, Portunus trituberculatus. Aquaculture, 430 : 166-172.
Li X Y, Wang J T, Han T, Hu S X, Jiang Y D, Wang C L. 2014b. Effects of phospholipid addition to diets with different inclusion levels of fish oil on growth and fatty acid body composition of juvenile swimming crab Portunus trituberculatus. Aquaculture Research, http://dx.doi. org/10.1111/are.12567.
Lim C, Ako H, Brown C L, Hahn K. 1997. Growth response and fatty acid composition of juvenile Penaeus vannamei fed different sources of dietary lipid. Aquaculture, 151 (1- 4): 143-153.
Lin H Z, Liu Y J, He J G, Zheng W H, Tian L X. 2007. Alternative vegetable lipid sources in diets for grouper, Epinephelus coioides (Hamilton): effects on growth, and muscle and liver fatty acid composition. Aquaculture Research, 38 (15): 1 605-1 611.
Meng X H, Jang I K, Seo H C, Cho Y R. 2009. White spot syndrome virus quantification in blue crab Portunus trituberculatus hatchery-produced larvae and wild populations by TaqMan real-time PCR, with an emphasis on the relationship between viral infection and crab health. Aquaculture, 2 9 1 (1-2): 18-22.
Menoyo D, Lopez-Bote C J, Diez A, Obach A, Bautista J M. 2007. Impact of n -3 fatty acid chain length and n -3/n -6 ratio in Atlantic salmon (Salmo salar) diets. Aquaculture, 267 (1-4): 248-259.
Merican Z O, Shim K. 1996. Qualitative requirements of essential fatty acids for juvenile Penaeus monodon. Aquaculture, 147 (3-4): 275-291.
Mourente G, Good J E, Bell J. 2005. Partial substitution of fish oil with rapeseed, linseed and olive oils in diets for European sea bass (Dicentrarchus labrax L.): effects on flesh fatty acid composition, plasma prostaglandins E2 and F, immune function and effectiveness of a fish oil finishing diet. Aquaculture Nutrition, 11 (1): 25-40.
Naylor R L, Hardy R W, Bureau D P, Chiu A, Elliott M, Farrell A P, Forster I, Gatlin D M, Goldburg R J, Hua K. 2009. Feeding aquaculture in an era of finite resources. Proceedings of the National Academy of Sciences of the United States of America, 106 (36): 15 103-15 110.
Olsen R, Myklebust R, Kaino T, Ringø E. 1999. Lipid digestibility and ultrastructural changes in the enterocytes of Arctic char (Salvelinus alpinus L.) fed linseed oil and soybean lecithin. Fish Physiology and Biochemistry, 21 (1): 35-44.
Ponat A, Adelung D. 1980. Studies to establish an optimal diet for Carcinus maenas II. Protein and lipid requirements. Marine Biology, 60 (2-3): 115-122.
Read G. 1981. The response of Penaeus indicus (Crustacea: Penaeidea) to purified and compounded diets of varying fatty acid composition. Aquaculture, 24 : 245-256.
Regost C, Arzel J, Robin J, Rosenlund G, Kaushik S J. 2003. Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima): 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture, 217 (1-4): 465-482.
Sargent J, Bell G, McEvoy L, Tocher D, Estevez A. 1999. Recent developments in the essential fatty acid nutrition of fish. Aquaculture, 177 (1-4): 191-199.
Sánchez D R, Fox J M, Gatlin III D, Lawrence A L. 2014. Dietary effect of fish oil and soybean lecithin on growth and survival of juvenile Litopenaeus vannamei in the presence or absence of phytoplankton in an indoor system. Aquaculture Research, 45 (8): 1 367-1 379.
Secor D H, Hines A H, Place A R. 2002. Japanese hatchery based stock enhancement: lessons for the Chesapeake Bay blue crab. University of Maryland Center for Environmental Science Chesapeake Biological Laboratory.
Shantha N, Ackman R. 1990. Nervonic acid versus tricosanoic acid as internal standards in quantitative gas chromatographic analyses of fish oil longer-chain n -3 polyunsaturated fatty acid methyl esters. Journal of Chromatography B : Biomedical Sciences and Applications, 533 : 1-10.
Shapawi R, Mustafa S, Ng W K. 2008. Effects of dietary fish oil replacement with vegetable oils on growth and tissue fatty acid composition of humpback grouper, Cromileptes altivelis (Valenciennes). Aquaculture Research, 39 (3): 315-323.
Sheen S S, Wu S W. 1999. The effects of dietary lipid levels on the growth response of juvenile mud crab Scylla serrata. Aquaculture, 175 (1-2): 143-153.
Suprayudi M A, Takeuchi T, Hamasaki K. 2004. Essential fatty acids for larval mud crab Scylla serrata : implications of lack of the ability to bioconvert C18 unsaturated fatty acids to highly unsaturated fatty acids. Aquaculture, 231 (1-4): 403-416.
Takeuchi T, Toyota M, Satoh S, Watanabe T. 1990. Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids. Nippon Suisan Gakkaishi, 56 (8): 1 263-1 269.
Thompson K R, Bailey T J, Metts L S, Brady Y J, Webster C D. 2010. Growth response and fatty acid composition of juvenile red claw crayfish (Cherax quadricarinatus) fed different sources of dietary lipid. Aquaculture Nutrition, 16 (6): 604-615.
Trushenski J T, Kasper C S, Kohler C C. 2006. Challenges and opportunities in finfish nutrition. North American Journal of Aquaculture, 68 (2): 122-140.
Turchini G M, Mentasti T, Frøyland L, Orban E, Caprino F, Moretti V M, Valfré F. 2003. Effects of alternative dietary lipid sources on performance, tissue chemical composition, mitochondrial fatty acid oxidation capabilities and sensory characteristics in brown trout (Salmo trutta L.). Aquaculture, 225 (1-4): 251-267.
Turchini G M, Torstensen B E, Ng W K. 2009. Fish oil replacement in finfish nutrition. Reviews in Aquaculture, 1 (1): 10-57.
Unnikrishnan U, Chakraborty K, Paulraj R. 2010. Efficacy of various lipid supplements in formulated pellet diets for juvenile Scylla serrata. Aquaculture Research, 41 (10): 1 498-1 513.
Valfré F, Caprino F, Turchini G M. 2003. The health benefit of seafood. Veterinary Research Communications, 27 (S1): 507-512.
Wang X X, Li Y J, Hou C L, Gao Y, Wang Y Z. 2012. Influence of different dietary lipid sources on the growth, tissue fatty acid composition, histological changes and peroxisome proliferator-activated receptor γ gene expression in large yellow croaker (Pseudosciaena crocea R.). Aquaculture Research, 43 (2): 281-291.
Watanabe T. 1982. Lipid nutrition in fish. Comparative Biochemistry and Physiology Part B : Comparative Biochemistry, 73 (1): 3-15.
Xu R, Hung S S, German J B. 1993. White sturgeon tissue fatty acid compositions are affected by dietary lipids. The Journal of Nutrition, 123 (10): 1 685-1 692. Xu X L, Ji W J, Castell J D, O'dor R K. 1994. Essential fatty acid requirement of the Chinese prawn, Penaeus chinensis. Aquaculture, 127 (1): 29-40.
Xue M, Luo L, Wu X F, Ren Z L, Gao P, Yu Y, Pearl G. 2006. Effects of six alternative lipid sources on growth and tissue fatty acid composition in Japanese sea bass (Lateolabrax japonicus). Aquaculture, 260 (1-4): 206-214.
Yu C G, Song H T, Yao G C, Shen X Y. 2003. Study on rational utilization of crab resources in the inshore waters of Zhejiang. Ma r ine Fish, 25 (3): 136-141. (in Chinese with English abstract)
Zhou Q C, Li C C, Liu C W, Chi S Y, Yang Q H. 2007. Effects of dietary lipid sources on growth and fatty acid composition of juvenile shrimp, Litopenaeus vannamei. Aquaculture Nutrition, 13 (3): 222-229.
Copyright © Haiyang Xuebao