Cite this paper:
Zhongshan ZHANG, Xiaomei WANG, Yongliang PAN, Zhanqi WANG, Zhengshun WEN, Feng LIU, Genxiang MAO. Pyropia haitanensis polysaccharide extends lifespan by inhibiting protein aggregation in Caenorhabditis elegans[J]. Journal of Oceanology and Limnology, 2021, 39(2): 705-713

Pyropia haitanensis polysaccharide extends lifespan by inhibiting protein aggregation in Caenorhabditis elegans
Zhongshan ZHANG1, Xiaomei WANG1, Yongliang PAN1, Zhanqi WANG1, Zhengshun WEN2, Feng LIU3, Genxiang MAO4
1 Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou Central Hospital, Huzhou 313000, China;
2 Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan 316022, China;
3 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
4 Zhejiang Provincial Key Lab of Geriatrics, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310013, China
Abstract:
Pyropia haitanensis polysaccharide (LP) have been found for having many excellent functions such as anti-aging. Using Caenorhabditis elegans models, we evaluated the anti-aging activity of LP by observing the lifespan, reproduction, pharyngeal pumping, stress response, quantitative fluorescence of polyglutamic acid, and nuclear localization of DAF-16 of worms. The results reveal that LP could extend the adult lifespan of wild-type and polyQ nematodes, indicating a connection of its anti-aging benefit with the toxicity-suppressing effect. The number of polyglutamic acid aggregates in high concentration groups decreased by 24.39% (P<0.05) to the control. The high-dose group strongly induced DAF-16 nuclear translocation over intermediate and cytosolic localizations compared with the control (P<0.001). Therefore, we believe that LP could extend the lifespan and reduce the protein aggregation in C. elegans through nuclear DAF-16::GFP expression.
Key words:    Pyropia haitanensis|polysaccharide|protein aggregation|Caenorhabditis elegans   
Received: 2020-02-14   Revised: 2020-04-13
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Articles by Zhongshan ZHANG
Articles by Xiaomei WANG
Articles by Yongliang PAN
Articles by Zhanqi WANG
Articles by Zhengshun WEN
Articles by Feng LIU
Articles by Genxiang MAO
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