Characterization of microbial communities in sediments of the South Yellow Sea -- Journal of Oceanology and Limnology,2021,39(3):846-864

	Cite this paper:
	Ye CHEN, Siqi LI, Xiaoqing XU, Manman MA, Tiezhu MI, Yu ZHEN, Zhigang YU. Characterization of microbial communities in sediments of the South Yellow Sea[J]. Journal of Oceanology and Limnology, 2021, 39(3): 846-864

Characterization of microbial communities in sediments of the South Yellow Sea

Ye CHEN^1,2,3, Siqi LI^1,2,3, Xiaoqing XU^3,4, Manman MA^2,3,4, Tiezhu MI^2,3,4, Yu ZHEN^2,3,4, Zhigang YU^2,5

1 College of Marine Life Science, Ocean University of China, Qingdao 266003, China;
2 Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;
3 Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China;
4 College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;
5 Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China

Abstract:

Illumina sequencing and quantitative PCR (qPCR) based on the 16S ribosomal RNA (rRNA) gene were conducted to characterize the vertical distribution of bacterial and archaeal communities in the sediments of two sites from the South Yellow Sea. Both bacterial and archaeal communities showed a clear stratified distribution with sediment depth. The microbial communities in the upper layers were distinct from those in the deeper layers; the relative abundances of sequences of Thaumarchaeota, Gammaproteobacteria, and Actinobacteria were higher in the upper than in the deeper sediments, whereas the sequences of Bathyarchaeia, Lokiarchaeota, Euryarchaeota, Chloroflexi, and Deltaproteobacteria were relatively more abundant in the deeper sediments. Sediment depth and total organic carbon (TOC) can significantly influence both the bacterial and archaeal communities. Furthermore, bacterial and archaeal groups potentially involved in nitrogen, sulfur, and methane metabolism were detected in both sites. In our study, both ammonia-oxidizing bacteria (Nitrospira) and ammonia-oxidizing archaea (Candidatus Nitrosopumilus) were responsible for ammonia oxidization. Additionally, sulfur-reducing bacteria SEEP-SRB1 forming consortia with anaerobic methane-oxidizing archaea ANME-2a-2b were capable of anaerobic methane oxidation (AOM) in the 3400-02 sediment samples.

Key words: microbial community|16S rRNA gene|high-throughput sequencing|South Yellow Sea|sediment

Received: 2020-03-03 Revised: 2020-04-06

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