Cite this paper:
CHEN Xianfeng, ZHOU Qianjin, DUAN Weijun, ZHOU Chengxu, DUAN Lijun, ZHANG Huili, SUN Aili, YAN Xiaojun, CHEN Jiong. Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters[J]. Journal of Oceanology and Limnology, 2016, 34(1): 86-101

Development and evaluation of a DNA microarray assay for the simultaneous detection of nine harmful algal species in ship ballast and seaport waters

CHEN Xianfeng1,2,3, ZHOU Qianjin1,2, DUAN Weijun3, ZHOU Chengxu2, DUAN Lijun3, ZHANG Huili3, SUN Aili1, YAN Xiaojun2, CHEN Jiong1,2
1 Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 The Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture, Ningbo University, Ningbo 315211, China;
3 Ningbo Academy of Inspection and Quarantine, Ningbo 315012, China
Abstract:
Rapid, high-throughput and reliable methods are urgently required to accurately detect and monitor harmful algae, which are responsible for algal blooms, such as red and green tides. In this study, we successfully developed a multiplex PCR-based DNA microarray method capable of detecting nine harmful algal species simultaneously, namely Alexandrium tamarense, Gyrodinium instriatum, Heterosigma akashiwo, Karenia mikimotoi, Prorocentrum donghaiense, Prorocentrum minimum, Ulva compressa, Ulva ohnoi and Ulva prolifera. This method achieved a limit of detection (LOD) of 0.5 ng of genomic DNA (orders of magnitude of the deci-nanogram range) in the tested algae cultures. Altogether, 230 fi eld samples from ship ballast waters and seaport waters were used to evaluate the DNA microarray. The clinical sensitivity and specifi city of the DNA microarray assay in detecting fi eld samples were 96.4% and 90.9%, respectively, relative to conventional morphological methods. This indicated that this high-throughput, automatic, and specifi c method is well suited for the detection of algae in water samples.
Key words:    ballast waters|DNA microarray|harmful algae|limit of detection|multiplex PCR|seaport waters   
Received: 2014-10-13   Revised: 2014-12-08
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Articles by CHEN Xianfeng
Articles by ZHOU Qianjin
Articles by DUAN Weijun
Articles by ZHOU Chengxu
Articles by DUAN Lijun
Articles by ZHANG Huili
Articles by SUN Aili
Articles by YAN Xiaojun
Articles by CHEN Jiong
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