Characterization and identifi cation of enzyme-producing microfl ora isolated from the gut of sea cucumber <i>Apostichopus japonicus</i> -- Journal of Oceanology and Limnology,2016,34(1):153-162

	Cite this paper:
	LI Fenghui, GAO Fei, TAN Jie, FAN Chaojing, SUN Huiling, YAN Jingping, CHEN Siqing, WANG Xiaojun. Characterization and identifi cation of enzyme-producing microfl ora isolated from the gut of sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2016, 34(1): 153-162

Characterization and identifi cation of enzyme-producing microfl ora isolated from the gut of sea cucumber Apostichopus japonicus

LI Fenghui^1,2, GAO Fei¹, TAN Jie¹, FAN Chaojing^1,2, SUN Huiling¹, YAN Jingping¹, CHEN Siqing¹, WANG Xiaojun³

1 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
3 Zhaoyuan Marine Environmental Monitoring Station, Yantai 265400, China

Abstract:

Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplifi ed with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microfl ora in the A. japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

Received: 2014-05-13 Revised: 2014-08-20

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Articles by LI Fenghui

Articles by GAO Fei

Articles by TAN Jie

Articles by FAN Chaojing

Articles by SUN Huiling

Articles by YAN Jingping

Articles by CHEN Siqing

Articles by WANG Xiaojun

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