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Cite this paper: |
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ZHANG Wenjing, SUN Delin, ZHAO Xia, JIN Weihua, WANG Jing, ZHANG Quanbin. Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica[J]. Journal of Oceanology and Limnology, 2016, 34(1): 177-185 |
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Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica |
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| ZHANG Wenjing1,2, SUN Delin3, ZHAO Xia4, JIN Weihua1, WANG Jing1, ZHANG Quanbin1 |
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1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Heze Junxinyuan Food Company, Heze 274415, China;
4 Ocean University of China, Qingdao 266071, China |
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| Abstract: |
| A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fl uorescence derivatization has been developed to determine the amount of low-molecularweight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fi t a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t1/2α)=11.24±2.93 min, half-time of elimination phase (t1/2β)=98.20±25.78 min, maximum concentration (Cmax)=110.53 μg/mL and peak time (Tmax)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection. |
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| Key words:
sulfated polysaccharide|HPLC post-column derivatization|microanalysis|pharmacokinetics
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| Received: 2014-10-20 Revised: 2015-01-03 |
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References:
Han Y H, Lv Z H, Jiang T F, Wang Y H. 2007. Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fl uorescence derivatization.Journal of Chromatography B, 845 (1): 138-142.
Honda S. 1996. Postcolumn derivatization for chromatographic analysis of carbohydrates. Journal of Chromatography A, 720 (1-2): 183-199.
Huang Y, Washio Y, Hara M, Toyoda H, Koshiishi I, Toida T,Imanari T. 1996. Simultaneous determination of dermatan sulfate and oversulfated dermatan sulfate in plasma by high-performance liquid chromatography with postcolumn fl uorescence derivatization. Analytical Biochemistry, 240 (2): 227-234.
Jin W H, Wang J, Ren S M, Song N, Zhang Q B. 2012.Structural analysis of a heteropolysaccharide from Saccharina japonica by electrospray mass spectrometry in tandem with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS). Marine Drugs, 10 (10): 2 138-2 152.
Jin W H, Zhang W J, Wang J, Ren S M, Song N, Zhang Q B. 2013. Structural analysis of heteropolysaccharide from Saccharina japonica and its derived oligosaccharides.International Journal of Biological Macromolecules, 62 : 697-704.
Millet J, Jouault S C, Mauray S, Theveniaux J, Sternberg C,Boisson Vidal C, Fischer A M. 1999. Antithrombotic and anticoagulant activities of a low molecular weight fucoidan by the subcutaneous route. Thrombosis and Haemostasis, 81 (3): 391-395.
Mizuno M, Nishitani Y, Tanoue T, Matoba Y, Ojima T,Hashimoto T, Kanazawa K. 2009. Quantifi cation and localization of fucoidan in Laminaria japonica using a novel antibody. Bioscience, Biotechnology, and Biochemistry, 73 (2): 335-338.
Olsson Y, Svensjö E, Arfors K E, Hultström D. 1975.Fluorescein labelled dextrans as tracers for vascular permeability studies in the nervous system. Acta Neuropathologica, 33 (1): 45-50.
Polson C, Sarkar P, Incledon B, Raguvaran V, Grant R. 2003.Optimization of protein precipitation based upon eff ectiveness of protein removal and ionization eff ect in liquid chromatography-tandem mass spectrometry.Journal of Chromatography B, 785 (2): 263-275.
Sun S M, Zhao X, Li G S, Yu G L, Xing X X, Zeng Y Y, Wu J,Wang J N. 2012. Microanalysis of oligosaccharide HS203 in beagle dog plasma by postcolumn fl uorescence derivatization method. Carbohydrate Polymers, 89 (2): 661-666.
Suzuki S, Fujimori T, Yodoshi M. 2006. Recovery of free oligosaccharides from derivatives labeled by reductive amination. Analytical Biochemistry, 354 (1): 94-103.
Toida T, Huang Y, Washio Y, Maruyama T, Toyoda H, Imanari T, Linhardt R J. 1997. Chemical microdetermination of heparin in plasma. Analytical Biochemistry, 251 (2): 219- 226.
Tokita Y, Nakajima K, Mochida H, Iha M, Nagamine T. 2010.Development of a fucoidan-specifi c antibody and measurement of fucoidan in serum and urine by sandwich ELISA. Bioscience, Biotechnology, and Biochemistry, 74 (2): 350-357.
Trento F, Cattaneo F, Pescador R, Porta R, Ferro L. 2001.Antithrombin activity of an algal polysaccharide.Thrombosis Research, 102 (5): 457-465.
Wang J, Wang F, Hou Y, Zhang H, Zhang Q B. 2012. Eff ect and mechanism of fucoidan derivatives from Laminaria japonica in experimental adenine-induced chronic kidney disease. Journal of Ethnopharmacology, 139 (3): 807-813.
Wang J, Zhang Q B, Zhang Z S, Li Z E. 2008. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 42 (2): 127-132.
Wang J, Zhang Q B, Zhang Z S, Song H F, Li P C. 2010a.Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. International Journal of Biological Macromolecules, 46 (1): 6-12.
Wang W T, Zhou J H, Xing S T, Guan H S. 1994.Immunomodulating action of marine algae sulfated polysaccharides on normal and immunosuppressed mice.Chinese Journal of Pharmacology and Toxicology, 8 (3): 199-199. (in Chinese with English abstract)
Wang Y, Yin H P, Lv X B, Wang Y F, Gao H Y, Wang M. 2010b. Protection of chronic renal failure by a polysaccharide from Cordyceps sinensis. Fitoterapia, 81 (5): 397-402.
Wijesekara I, Pangestuti R, Kim S K. 2011. Biological activities and potential health benefi ts of sulfated polysaccharides derived from marine algae. Carbohydrate Polymers, 84 (1): 14-21.
Zhang J J, Zhang Q B, Wang J, Shi X L, Zhang Z S. 2009.Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC. Chinese Journal of Oceanology and Limnology, 27 (3): 578-582. (in Chinese with English abstract)
Zhang W J, Wang J, Jin W H, Zhang Q B. 2013. The antioxidant activities and neuroprotective eff ect of polysaccharides from the starfi sh Asterias rollestoni. Carbohydrate Polymers, 95 (1): 9-15.
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