Isolation and characterization of bioactive fungi from shark <i>Carcharodon carcharias</i>' gill with biopharmaceutical prospects -- Journal of Oceanology and Limnology,2016,34(1):186-199

	Cite this paper:
	ZHANG Yi, HAN Jinyuan, FENG Yan, MU Jun, BAO Haiyan, Andreas KULIK, Stephanie GROND. Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects[J]. Journal of Oceanology and Limnology, 2016, 34(1): 186-199

Isolation and characterization of bioactive fungi from shark Carcharodon carcharias' gill with biopharmaceutical prospects

ZHANG Yi^1,2,3,4, HAN Jinyuan², FENG Yan¹, MU Jun⁵, BAO Haiyan², Andreas KULIK⁶, Stephanie GROND⁴

1 College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China;
2 School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian 116028, China;
3 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;
4 Institute of Organic Chemistry, University of T黚ingen, T黚ingen 72076, Germany;
5 Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, China;
6 Inter-Faculty Institute for Microbiology and Infectious Medicine, University of T黚ingen, T黚ingen 72076, Germany

Abstract:

Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark (Carcharodon carcharias). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated signifi cant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit (ITS-LSU) rDNA-sequence BLAST comparison. Four genera, Penicillium , Aspergillus , Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profi les. These results detail the broad range of bioactive fungi found in a shark’s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the fi rst study characterizing shark gill fungi and their bioactivity.

Received: 2014-07-17 Revised: 2014-12-01

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Articles by Stephanie GROND

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