Cite this paper:
ZHOU Jun, HOU Fujing, LI Ye, SU Xiurong, LI Taiwu, JIN Chunhua. Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide[J]. Journal of Oceanology and Limnology, 2016, 34(4): 719-729

Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

ZHOU Jun1, HOU Fujing2, LI Ye1, SU Xiurong1, LI Taiwu3, JIN Chunhua1
1 School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 Ningbo Green-Health Pharmaceutical Co. Ltd., Ningbo 315505, China;
3 Ningbo City College of Vocational Technology, Ningbo 315100, China
Abstract:
Acaudina leucoprocta is an edible sea cucumber of economic interest that is widely distributed in China. Little information is available concerning the molecular genetics of this species although such knowledge would contribute to a better understanding of the optimal conditions for its aquaculture and its mechanisms of defense against disease. Therefore, we constructed a cDNA library and, based on bioinformatics analysis of the sequences, the functions of 75% of the cDNAs were identified, including those involved in cell structure, energy metabolism, mitochondrial function, and signal transduction pathways. Approximately 25% of genes in the library were unmatched. The gene for A. leucoprocta ferritin was also cloned. The predicted amino-acid sequence of ferritin displayed significant homology with other sea-cucumber counterparts but indicated that it was a new member of the ferritin family. Semiquantitative real-time RT-PCR indicated the highest levels of ferritin mRNA expression in the intestine. A polyclonal antibody of ferritin was also produced. These data provide a set of molecular tools essential for further studies of the functions of ferritin protein in A. leucoprocta.
Key words:    Acaudina leucoprocta|cDNA library|ferritin|real-time RT-PCR|polyclonal antibody   
Received: 2015-01-10   Revised: 2015-02-25
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Articles by ZHOU Jun
Articles by HOU Fujing
Articles by LI Ye
Articles by SU Xiurong
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Articles by JIN Chunhua
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