Production of γ-linolenic acid and stearidonic acid by <i>Synechococcus</i> sp. PCC7002 containing cyanobacterial fatty acid desaturase genes -- Journal of Oceanology and Limnology,2016,34(4):772-780

	Cite this paper:
	DONG Xuewei, HE Qingfang, PENG Zhenying, YU Jinhui, BIAN Fei, LI Youzhi, BI Yuping. Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes[J]. Journal of Oceanology and Limnology, 2016, 34(4): 772-780

Production of γ-linolenic acid and stearidonic acid by Synechococcus sp. PCC7002 containing cyanobacterial fatty acid desaturase genes

DONG Xuewei¹, HE Qingfang^2,3, PENG Zhenying², YU Jinhui², BIAN Fei², LI Youzhi¹, BI Yuping²

1 College of Life Science and Technology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China;
2 Biotechnology Research Center, Shandong Academy of Agricultural Science, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China;
3 Department of Applied Science, University of Arkansas, Little Rock, Arkansas 72204, US

Abstract:

Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene and is therefore unable to synthesize γ-linolenic acid (GLA) and stearidonic acid (SDA), which are important in human health. In this work, we constructed recombinant vectors Syd6D, Syd15D and Syd6Dd15D to express the Δ15 desaturase and Δ6 desaturase genes from Synechocystis PCC6803 in Synechococcus sp. PCC7002, with the aim of expressing polyunsaturated fatty acids. Overexpression of the Δ15 desaturase gene in Synechococcus resulted in 5.4 times greater accumulation of α-linolenic acid compared with the wild-type while Δ6 desaturase gene expression produced both GLA and SDA. Co-expression of the two genes resulted in low-level accumulation of GLA but much larger amounts of SDA, accounting for as much to 11.64% of the total fatty acid content.

Key words: Synechococcus sp. PCC7002|Synechocystis sp. PCC6803|Δ15 fatty acid desaturase|Δ6 fatty acid desaturase|polyunsaturated fatty acids

Received: 2014-12-15 Revised: 2015-02-25

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