Screening and isolation of the algicidal compounds from marine green alga <i>Ulva intestinalis</i> -- Journal of Oceanology and Limnology,2016,34(4):781-788

	Cite this paper:
	SUN Xue, JIN Haoliang, ZHANG Lin, HU Wei, LI Yahe, XU Nianjun. Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis[J]. Journal of Oceanology and Limnology, 2016, 34(4): 781-788

Screening and isolation of the algicidal compounds from marine green alga Ulva intestinalis

SUN Xue, JIN Haoliang, ZHANG Lin, HU Wei, LI Yahe, XU Nianjun

Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, China

Abstract:

Twenty species of seaweed were collected from the coast of Zhejiang, China, extracted with ethanol, and screened for algicidal activity against red tide microalgae Heterosigma akashiwo and Prorocentrum micans. Inhibitory effects of fresh and dried tissues of green alga Ulva intestinalis were assessed and the main algicidal compounds were isolated, purified, and identified. Five seaweed species, U. intestinalis, U. fasciata, Grateloupia romosissima, Chondria crassicaulis, and Gracilariopsis lemaneiformis, were investigated for their algicidal activities. Fresh tissues of 8.0 and 16.0 mg/mL of U. intestinalis dissolved in media significantly inhibited growth of H. akashiwo and P. micans, respectively. Dried tissue and ethyl acetate (EtOAc) extracts of U. intestinalis at greater than 1.2 and 0.04 mg/mL, respectively, were fatal to H. akashiwo, while its water and EtOAc extracts in excess of 0.96 and 0.32 mg/mL, respectively, were lethal to P. micans. Three algicidal compounds in the EtOAc extracts were identified as 15-ethoxy-(6z,9z,12z)-hexadecatrienoic acid (I), (6E,9E,12E)-(2-acetoxy-β-D-glucose)-octadecatrienoic acid ester (II) and hexadecanoic acid (III). Of these, compound II displayed the most potent algicidal activity with IC₅₀ values of 4.9 and 14.1 μg/mL for H. akashiwo and P. micans, respectively. Compound I showed moderate algicidal activity with IC₅₀ values of 13.4 and 24.7 μg/mL for H. akashiwo and P. micans, respectively. These findings suggested that certain macroalgae or products therefrom could be used as effective biological control agents against red tide algae.

Key words: Ulva intestinalis|inhibitory effect|red tide algae|Heterosigma akashiwo|Prorocentrum micans

Received: 2015-01-06 Revised: 2015-05-04

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Articles by SUN Xue

Articles by JIN Haoliang

Articles by ZHANG Lin

Articles by HU Wei

Articles by LI Yahe

Articles by XU Nianjun

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