Histological change and heat shock protein 70 expression in different tissues of Japanese flounder <i>Paralichthys olivaceus</i> in response to elevated temperature -- Journal of Oceanology and Limnology,2015,33(1):11-19

	Cite this paper:
	LIU Yifan, MA Daoyuan, XIAO Zhizhong, XU Shihong, WANG Yanfeng, WANG Yufu, XIAO Yongshuang, SONG Zongcheng, TENG Zhaojun, LIU Qinghua, LI Jun. Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature[J]. Journal of Oceanology and Limnology, 2015, 33(1): 11-19

Histological change and heat shock protein 70 expression in different tissues of Japanese flounder Paralichthys olivaceus in response to elevated temperature

LIU Yifan^1,2, MA Daoyuan¹, XIAO Zhizhong¹, XU Shihong¹, WANG Yanfeng¹, WANG Yufu^1,2, XIAO Yongshuang^1,3, SONG Zongcheng⁴, TENG Zhaojun⁵, LIU Qinghua¹, LI Jun¹

1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong 226019, China;
4 Weihai Shenghang Aquatic Product Science and Technology Co. Ltd., Weihai 264200, China;
5 Rizhao Proliferation Station of Marine Aquatic Resources, Rizhao 276805, China

Abstract:

High temperature influences the homeostasis of fish. We investigated the effects of elevated temperature on tissues of Japanese flounder (Paralichthys olivaceus) by analyzing the histology and heat shock protein 70 (hsp70) expression of fish reared in warm conditions. In this study, temperature was increased at 1±0.5℃/day starting at 24±0.5℃, and was kept at that temperature for 5 days before the next rise. After raising temperature at the rate up to 32±0.5℃, tissue samples from midgut, spleen, stomach, liver, muscle, gill, heart, trunk kidney and brain were collected for histological analysis and mRNA assay. Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃. Histological assessment of the tissues indicated that the gill had the most serious damage, including highly severe epithelial lifting and edema, curved tips and hyperemia at the ending of the lamellars, desquamation and necrosis. The next most severe damage was found in liver and kidney. The hsp70 levels in all the tissues first increased and then decreased. The gut, stomach, muscle, heart, and brain had the highest expressions in 6 h, whereas the spleen, liver, gill and kidney had the highest expressions in 2 h. Therefore, tissues with the most significant lesions (especially gill and liver) responded much earlier (2 h) in hsp70 expression than other tissues, and these tissues demonstrated the most marked histological disruption and elevated mRNA levels, making them ideal candidates for further studies on the thermal physiology of this species.

Key words: temperature|Paralichthys olivaceus|histology|hsp70|tissue expression

Received: 2014-01-28 Revised: 2014-04-09

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