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REN Wei, WANG Shujun, LÜ Mingsheng, WANG Xiaobei, FANG Yaowei, JIAO Yuliang, HU Jianen. Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash[J]. Journal of Oceanology and Limnology, 2016, 34(2): 354-366

Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash

REN Wei1,2, WANG Shujun2,3,4, LÜ Mingsheng2,3,4, WANG Xiaobei2,4, FANG Yaowei2,3,4, JIAO Yuliang2,3,4, HU Jianen1
1 College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China;
2 Jiangsu Marine Resources Development Research Institute, Lianyungang 222005, China;
3 Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China;
4 Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China
We adopted the response surface methodology using single factor and orthogonal experiments to optimize four types of antimicrobial agents that could inhibit biofi lm formation by Streptococcus mutans, which is commonly found in the human oral cavity and causes tooth decay. The objective was to improve the function of marine Arthrobacter oxydans KQ11 dextranase mouthwash(designed and developed by our laboratory). The experiment was conducted in a three-level, four-variable central composite design to determine the best combination of ZnSO4, lysozyme, citric acid and chitosan. The optimized antibacterial agents were 2.16 g/L ZnSO4, 14 g/L lysozyme, 4.5 g/L citric acid and 5 g/L chitosan. The biofi lm formation inhibition reached 84.49%. In addition, microscopic observation of the biofi lm was performed using scanning electron microscopy and confocal laser scanning microscopy. The optimized formula was tested in marine dextranase Arthrobacter oxydans KQ11 mouthwash and enhanced the inhibition of S. mutans. This work may be promoted for the design and development of future marine dextranase oral care products.
Key words:    antimicrobial agent|marine dextranase mouthwash|response surface methodology|biofi lm|scanning electron microscopy|confocal laser scanning microscopy   
Received: 2014-12-13   Revised: 2015-03-27
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