Investigation into the hemolytic activity of tentacle venom from jellyfi sh <i>Cyanea nozakii</i> Kishinouye -- Journal of Oceanology and Limnology,2016,34(2):382-385

	Cite this paper:
	LI Cuiping, YU Huahua, LI Rongfeng, XING Ronge, LIU Song, LI Pengcheng. Investigation into the hemolytic activity of tentacle venom from jellyfi sh Cyanea nozakii Kishinouye[J]. Journal of Oceanology and Limnology, 2016, 34(2): 382-385

Investigation into the hemolytic activity of tentacle venom from jellyfi sh Cyanea nozakii Kishinouye

LI Cuiping², YU Huahua¹, LI Rongfeng¹, XING Ronge¹, LIU Song¹, LI Pengcheng¹

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Qingdao University of Technology, Qingdao 266300, China

Abstract:

Cyanea nozakii Kishinouy e(C. nozakii), a giant cnidarian of the class Scyphomedusae, order Semaeostomeae and family Cyaneidae, is widely distributed in the East China Sea, the Yellow Sea and the Bohai Sea, and is abundant from late summer to early autumn. Venom produced by C. nozakii during mass agglomerations can contaminate seawater resulting in death of the halobios and seriously damage commercial fisheries. Swimmers and fishermen commonly suffer painful stings from this jellyfish, resulting in local edema, tingling, breathing difficulties, depressed blood pressure and even death. Such effects arise from the complex mixture of biologically active molecules that make up jellyfish venom. In the present study, the hemolytic activity of venom from tentacles of C. nozakii and factors affecting its activity were assayed. The HU₅₀(defined as the amount of protein required to lyse 50% of erythrocytes) of the venom against dove and chicken erythrocytes was 34 and 59μg/mL, respectively. Carboxylmethyl chitosan and glycerol could increase hemolytic activity at concentrations greater than 0.06% and 0.2 mol/L, respectively.

Key words: jellyfi sh|hemolytic activity|venom|Cyanea nozakii Kishinouye

Received: 2014-11-18 Revised: 2015-02-06

Tools

PDF (284 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by LI Cuiping

Articles by YU Huahua

Articles by LI Rongfeng

Articles by XING Ronge

Articles by LIU Song

Articles by LI Pengcheng

References:
Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2):248-254.
Chung J J, Ratnapala L A, Cooke I M, Yanagihara A A. 2001.Partial purification and characterization of a hemolysin(CAH1) from Hawaiian box jellyfish(Carybdea alata)venom. Toxicon, 39(7):981-990.
Dong J, Liu C Y, Li W Q, Yu H B, Wang B, Wang Y Q. 2005.The morphology and structure of jellyfish(Cyanea nozakii Kishinouye). Fish. Sci., 24(2):22-23.(in Chinese with English abstract)
Feng J H, Yu H H, Li C P, Xing R E, Liu S, Wang L, Cai S B, Li P C. 2010a. Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye. Toxicon, 55(1):118-125.
Feng J H, Yu H H, Xing R E, Liu S, Wang L, Cai S B, Li P C. 2010b. Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye. Toxico l. in Vitro, 24(6):1 750-1 756.
Gusmani L, Avian M, Galil B, Patriarca P, Rottini G. 1997.Biologically active polypeptides in the venom of the jellyfish Rhopilema nomadica. Toxicon, 35(5):637-648.
Li C P, Yu H H, Liu S, Xing R E, Guo Z Y, Li P C. 2005.Factors aff ecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg. Med.Chem. Lett., 15(24):5 370-5 374.
Li R F, Yu H H, Xing R E, Liu S, Qing Y K, Li K C, Li B, Meng X T, Cui J H, Li P C. 2013. Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris.Toxicol. in Vitro., 27(6):1 620-1 625.
Radwan F F Y, Burnett J W, Bloom D A, Coliano T, Eldefrawi M E, Erderly H, Aurelian L, Torres M, Heimer-de la Cotera E P. 2001. A comparison of the toxinological characteristics of two Cassiopea and Aurelia species.Toxicon, 39(2-3):245-257.
Radwan F F Y, Gershwin L A, Burnett J W. 2000. Toxinological studies on the nematocyst venom of Chrysaora achlyos.Toxicon, 38(11):1 581-1 591.
Radwan F F Y, Román L G, Baksi K, Burnett J W. 2005.Toxicity and mAChRs binding activity of Cassiopea xamachana venom from Puerto Rican coasts. Toxicon, 45(1):107-112.
Ramasamy S, Isbister G K, Seymour J E, Hodgson W C. 2003.The in vitro effects of two chirodropid(Chironex fl eckeri and Chiropsalmus sp.) venoms:effi cacy of box jellyfish antivenom. Toxicon, 41(6):703-711.
Rottini G, Gusmani L, Parovel E, Avian M, Patriarca P. 1995.Purification and properties of a cytolytic toxin in venom of the jellyfish Carybdea marsupi a lis. Toxicon, 33(3):315-326.
Torres M, Aguilar M B, Falcón A, Sánchez L, Radwan F F Y, Burnett J W, Heimer-de la Cotera E P, Arellano R O. 2001.Electrophysiological and hemolytic activity elicited by the venom of the jellyfish Cassiopea xamachana. Toxicon, 39(9):1 297-1 307.
Uechi G, Toma H, Arakawa T, Sato Y. 2005. Biochemical and physiological analyses of a hemolytic toxin isolated from a sea anemone Actineria villosa. Toxicon, 45(6):761-766.
Winter K L, Isbister G K, Seymour J E, Hodgson WC. 2007.An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fl eckeri. Toxicon, 49(6):804-809.
Xiao L, He Q, Guo Y F, Zhang J, Nie F, Li Y, Ye X F, Zhang L M. 2009. Cyanea capillata tentacle-only extract as a potential alternative of nematocyst venom:its cardiovascular toxicity and tolerance to isolation and purification procedures. Toxicon, 53(1):146-152.
Yu H H, Li C P, Li R G, Xing R E, Liu S, Li P C. 2007. Factors infl uencing hemolytic activity of venom from the jellyfish Rhopilema Esculentum Kishinouye. Food Chem. Toxico l., 45(7):1 173-1 178.
Yu H H, Li R F, Dong X L, Xing R E, Liu S, Li P C. 2014.Effi cacy of venom from tentacle of jellyfish Stomolophus meleagris(Nemopilema nomurai) against the cotton bollworm Helicoverpa armigera. Biomed Res. Int., 2014:315853, http://dx.doi.org/10.1155/2014/315853.
Yu H H, Liu X G, Dong X L, Li C P, Xing R E, Liu S, Li P C. 2005. Insecticidal activity of proteinous venom from tentacle of jellyfish Rhopilema esculentum Kishinouye.Bioorg. Med. Chem. Lett., 15(22):4 949-4 952.
Yu H H, Liu X G, Xing R E, Guo Zh Y, Wang P B, Li C P, Li P C. 2006. In vitro determination of antioxidant activity of proteins from jellyfish Rhopilema esculentum. Food Chem., 95(1):123-130.
Zhong X M, Tang J H, Liu P T. 2004. A study on the relationship between Cyanea nozakii Kishinouye breaking out and ocean ecosystem. Mod. Fish. Inf., 19(3):15-17.(in Chinese with English abstract)