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LIU Jiao, LI Xianchao, TANG Xuexi, ZHOU Bin. Identifi cation and characterization of a DnaJ gene from red alga Pyropia yezoensis(Bangiales, Rhodophyta)[J]. Journal of Oceanology and Limnology, 2016, 34(2): 405-411

Identifi cation and characterization of a DnaJ gene from red alga Pyropia yezoensis(Bangiales, Rhodophyta)

LIU Jiao, LI Xianchao, TANG Xuexi, ZHOU Bin
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
Members of the DnaJ family are proteins that play a pivotal role in various cellular processes, such as protein folding, protein transport and cellular responses to stress. In the present study, we identified and characterized the full-length DnaJ cDNA sequence from expressed sequence tags of Pyropia yezoensis(PyDnaJ) via rapid identification of cDNA ends. This cDNA encoded a protein of 429 amino acids, which shared high sequence similarity with other identified DnaJ proteins, such as a heat shock protein 40/DnaJ from Pyropia haitanensis. The relative mRNA expression level of PyDnaJ was investigated using real-time PCR to determine its specific expression during the algal life cycle and during desiccation. The relative mRNA expression level in sporophytes was higher than that in gametophytes and significantly increased during the whole desiccation process. These results indicate that PyDnaJ is an authentic member of the DnaJ family in plants and red algae and might play a pivotal role in mitigating damage to P. yezoensis during desiccation.
Key words:    DnaJ|desiccation|life cycle|mRNA expression|Pyropia yezoensis   
Received: 2014-11-17   Revised: 2015-01-26
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