Long-term effects of di-octyl phthalate on the expression of immune-related genes in <i>Tegillarca granosa</i> -- Journal of Oceanology and Limnology,2016,34(3):423-429

	Cite this paper:
	WANG Ji, LI Ye, DAI Juan, SU Xiurong, LI Chenghua, SHEN Lingling. Long-term effects of di-octyl phthalate on the expression of immune-related genes in Tegillarca granosa[J]. Journal of Oceanology and Limnology, 2016, 34(3): 423-429

Long-term effects of di-octyl phthalate on the expression of immune-related genes in Tegillarca granosa

WANG Ji, LI Ye, DAI Juan, SU Xiurong, LI Chenghua, SHEN Lingling

School of Marine Sciences, Ningbo University, Ningbo 315211, China

Abstract:

Di-octyl phthalate (DOP) is widely used as a plasticizer in the plastics industry. As a result, DOP is often found in marine water ecosystems where many species are exposed to it. Our objective was to evaluate the effect of long-term (14 d) DOP exposure (2.6, 7.8, or 31.2 mg/L) on the expression of immunerelated genes in Tegillarca granosa. The expression of small heat shock protein (sHSPs) and tissue inhibitor of metalloproteinase (TIMP) were highest in clams exposed to 31.2 mg/L DOP on days 7 and 14. The relative expression of Tg-ferritin, superoxide dismutase (SOD), and metallothionein (MT) increased initially then decreased as the concentration of DOP increased. The hemoglobin of T. granosa (Tg-HbI) exhibited two distinct expression patterns at two time points. Our results suggest that the immune response of T. granosa against DOP pollution varies depending on the dose. Additionally, we identified some immune-related genes that are promising candidates for biomarkers of DOP.

Key words: Tegillarca granosa|di-octyl phthalate|immune response

Received: 2014-11-24 Revised: 2015-01-30

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Articles by WANG Ji

Articles by LI Ye

Articles by DAI Juan

Articles by SU Xiurong

Articles by LI Chenghua

Articles by SHEN Lingling

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