Significant population genetic structure detected in the rock bream <i>Oplegnathus fasciatus</i> (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis -- Journal of Oceanology and Limnology,2016,34(3):441-450

	Cite this paper:
	XIAO Yongshuang, MA Daoyuan, XU Shihong, LIU Qinghua, WANG Yanfeng, XIAO Zhizhong, LI Jun. Significant population genetic structure detected in the rock bream Oplegnathus fasciatus (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis[J]. Journal of Oceanology and Limnology, 2016, 34(3): 441-450

Significant population genetic structure detected in the rock bream Oplegnathus fasciatus (Temminck & Schlegel, 1844) inferred from fluorescent-AFLP analysis

XIAO Yongshuang^1,2, MA Daoyuan¹, XU Shihong¹, LIU Qinghua¹, WANG Yanfeng¹, XIAO Zhizhong¹, LI Jun¹

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Nantong Branch, Institute of Oceanology, Chinese Academy of Sciences, Nantong 226004, China

Abstract:

Oplegnathus fasciatus (rock bream) is a commercial rocky reef fish species in East Asia that has been considered for aquaculture. We estimated the population genetic diversity and population structure of the species along the coastal waters of China using fluorescent-amplifi ed fragment length polymorphisms technology. Using 53 individuals from three populations and four pairs of selective primers, we amplified 1264 bands, 98.73% of which were polymorphic. The Zhoushan population showed the highest Nei's genetic diversity and Shannon genetic diversity. The results of analysis of molecular variance (AMOVA) showed that 59.55% of genetic variation existed among populations and 40.45% occurred within populations, which indicated that a signifi cant population genetic structure existed in the species. The pairwise fixation index F_st ranged from 0.20 to 0.63 and were signifi cant after sequential Bonferroni correction. The topology of an unweighted pair group method with arithmetic mean tree showed two signifi cant genealogical branches corresponding to the sampling locations of North and South China. The AMOVA and STRUCTURE analyses suggested that the O. fasciatus populations examined should comprise two stocks.

Key words: Oplegnathus fasciatus|fluorescent-AFLP|genetic diversity|genetic structure

Received: 2014-12-31 Revised: 2015-03-03

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Articles by XIAO Yongshuang

Articles by MA Daoyuan

Articles by XU Shihong

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Articles by WANG Yanfeng

Articles by XIAO Zhizhong

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