Identifi cation and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of <i>Pyropia haitanensis</i> -- Journal of Oceanology and Limnology,2016,34(3):451-459

	Cite this paper:
	HUANG Aiyou, WANG Guangce. Identifi cation and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of Pyropia haitanensis[J]. Journal of Oceanology and Limnology, 2016, 34(3): 451-459

Identifi cation and bioinformatics analysis of microRNAs from the sporophyte and gametophyte of Pyropia haitanensis

HUANG Aiyou, WANG Guangce

Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Abstract:

Pyropia haitanensis (T. J. Chang et B. F. Zheng) N. Kikuchi et M. Miyata (Porphyra haitanensis) is an economically important genus that is cultured widely in China. P. haitanensis is cultured on a larger scale than Pyropia yezoensis, making up an important part of the total production of cultivated Pyropia in China. However, the majority of molecular mechanisms underlying the physiological processes of P. haitanensis remain unknown. P. haitanensis could utilize inorganic carbon and the sporophytes of P. haitanensis might possess a PCK-type C₄-like carbon-fi xation pathway. To identify microRNAs and their probable roles in sporophyte and gametophyte development, we constructed and sequenced small RNA libraries from sporophytes and gametophytes of P. haitanensis. Five microRNAs were identifi ed that shared no sequence homology with known microRNAs. Our results indicated that P. haitanensis might posses a complex sRNA processing system in which the novel microRNAs act as important regulators of the development of diff erent generations of P. haitanensis.

Key words: microRNA|Pyropia haitanensis|small RNA|sporophyte|gametophyte

Received: 2014-08-29 Revised: 2015-01-15

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Articles by HUANG Aiyou

Articles by WANG Guangce

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