An outbreak of granulomatous infl ammation associated with Francisella noatunensis subsp. orientalis in farmed tilapia (Oreochromis niloticus×O. aureus) in China -- Journal of Oceanology and Limnology,2016,34(3):460-466

	Cite this paper:
	LIN Qiang, LI Ningqiu, FU Xiaozhe, HU Qiandong, CHANG Ouqin, LIU Lihui, ZHANG Defeng, WANG Guangjun, SAN Guibao, WU Shuqin. An outbreak of granulomatous infl ammation associated with Francisella noatunensis subsp. orientalis in farmed tilapia (Oreochromis niloticus×O. aureus) in China[J]. Journal of Oceanology and Limnology, 2016, 34(3): 460-466

An outbreak of granulomatous infl ammation associated with Francisella noatunensis subsp. orientalis in farmed tilapia (Oreochromis niloticus×O. aureus) in China

LIN Qiang^1,2, LI Ningqiu^1,2, FU Xiaozhe^1,2, HU Qiandong¹, CHANG Ouqin¹, LIU Lihui^1,2, ZHANG Defeng^1,2, WANG Guangjun¹, SAN Guibao³, WU Shuqin^1,2

1 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Guangzhou 510380, China;
2 Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China;
3 Panyu District Agricultural Science Research Institute, Guangdong Province, Guangzhou 511450, China

Abstract:

In 2013, a novel disease was detected in tilapia (Oreochromis niloticus×O. aureus) in Guangzhou, South China. To identify the causative pathogen, we conducted histological examination, bacteria isolation, and purifi cation, and sequenced the 16S rRNA gene of isolates. Infected fish had a large number of white granulomas in the kidney, liver, heart, and spleen. The head kidney and spleen were markedly swollen. A bacterium strain designated as gz201301 was recovered from the spleen of infected tilapia. The 16S rRNA sequence of gz201301 revealed that it was highly similar to F. noatunensis subsp. orientalis. This is the first report of a Francisella-like infection in farmed tilapia in China.

Key words: granulomatous|tilapia|Francisella|16s rRNA

Received: 2014-11-14 Revised: 2015-02-06

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References:
Antuofermo E, Pais A, Nuvoli S, Hetzel U, Burrai G P, Rocca S, Caff ara M, Giorgi I, Pedron C, Prearo M. 2014. Mycobacterium chelonae associated with tumor-like skin and oral masses in farmed Russian sturgeons (Acipenser gueldenstaedtii). BMC Vet. Res., 10(1):18, http://dx.doi.org/10.1186/1746-6148-10-18.
Aro L, Correa K, Martínez A, Ildefonso R, Yáñez J M. 2014. Characterization of Mycobacterium salmoniphilum as causal agent of mycobacteriosis in Atlantic salmon, Salmo salar L., from a freshwater recirculation system. J. Fish Dis., 37(4):341-348, http://dx.doi.org/10.1111/jfd.12108.
Birkbeck T H, Bordevik M, Frøystad M K, Baklien Å. 2007. Identifi cation of Francisella sp. from Atlantic salmon, Salmo salar L., in Chile. J. Fish Dis., 30(8):505-507, http://dx.doi.org/10.1111/j.1365-2761.2007.00837.x.
Birkbeck T H, Feist S W, Verner-Jeff reys D W. 2011. Francisella infections in fish and shellfish. J. Fish Dis., 34(3):173-187, http://dx.doi.org/10.1111/j.1365-2761.2010.01226.x.
Chen S C, Tung M C, Chen S P, Tsai J F, Wang P C, Chen R S, Lin S C, Adams A. 1994. Systematic granulomas caused by a rickettsia-like organism in Nile tilapia, Oreochronuis niloticus (L.), from southern Taiwan. Journal of Fish Diseases, 17(6):591-599, http://dx.doi.org/10.1046/j.1365-2761.2003.00460.x.
Chern R S, Chao C B. 1994. Outbreaks of a disease caused by rickettsia-like organism in cultured tilapias[Oreochromis spp. and Tilapia spp.] in Taiwan. Fish Pathology, 29(2):61-71.
Colquhoun D J, Duodu S. 2011. Francisella infections in farmed and wild aquatic organisms. Vet. Res., 42(45):1-15, http://dx.doi.org/10.1186/1297-9716-42-47.
Fryer J L, Hedrick R P. 2003. Piscirickettsia salmonis:a Gramnegative intracellular bacterial pathogen of fish. J. Fish Dis., 26(5):251-262, http://dx.doi.org/10.1046/j.1365-2761.2003.00460.x
Goodwin A E, Lochmann R T, Tieman D M, Mitchell A J. 2002. Massive hepatic necrosis and nodular regeneration in largemouth bass fed diets high in available carbohydrate. Journal of the World Aquaculture Society, 33(4):466-477.
Gudmundsdottir B K, Gudmundsdottir S, Gudmundsdottir S, Magnadottir B. 2014. Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions. J. Fish Dis., 37(6):511-519, http://dx.doi.org/10.1111/jfd.12139.
Hsieh C Y, Tung M C, Tu C, Chang C D, Tsai S S. 2006. Enzootics of visceral granulomas associated with Francisella-like organism infection in tilapia(Oreochromis spp.). Aquaculture, 254(1-4):129-138, http://dx.doi.org/10.1016/j.aquaculture.2006.03.044.
Hsieh C Y, Wu Z B, Tung M C, Tsai S S. 2007. PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids. Dis. Aquat. Organ., 75:29-36, http://dx.doi.org/10.3354/dao075029.
Jeff ery K R, Stone D, Feist S W, Verner-Jeff reys D W. 2010. An outbreak of disease caused by Francisella sp. in Nile tilapia Oreochromis niloticus at a recirculation fish farm in the UK. Dis. Aquat. Organ., 91(2):161-165, http://dx.doi.org/10.3354/dao02260.
Kamaishi T, Fukuda Y, Nishiyama M, Kawakami H, Matsuyama T, Yoshinaga T, Oseko N. 2005. Identifi cation and pathogenicity of intracellular Francisella bacterium in three-line grunt Parapristipoma trilineatum. Fish Pathology, 40(2):67-72.
Kamaishi T, Miwa S, Goto E, Matsuyama T, Oseko N. 2010. Mass mortality of giant abalone Haliotis gigantea caused by a Francisella sp. bacterium. Dis. Aquat. Organ., 89(2):145-154, http://dx.doi.org/10.3354/dao02188.
Liu J Y, Li A H. 2012. First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China. J. Fish Dis., 35(5):335-342, http://dx.doi.org/10.1111/j.1365-2761.2012.01350.x.
Mauel M J, Miller D L, Styer E, Pouder D B, Yanong R P E, Goodwin A E, Schwedler T E. 2005. Occurrence of Piscirickettsiosis-like syndrome in tilapia in the continental United States. Journal of Veterinary Diagnostic Investigation, 17(6):601-605, http://dx.doi.org/10.1177/104063870501700616.
Mauel M J, Soto E, Moralis J A, Hawke J. 2007. A piscirickettsiosis-like syndrome in cultured Nile tilapia in Latin America with Francisella spp. as the pathogenic agent. J. Aquat. Anim. Health, 19(1):27-34, http://dx.doi.org/10.1577/H06-025.1.
Mikalsen J, Olsen A B, Tengs T, Colquhoun D J. 2007. Francisella philomiragia subsp. noatunensis subsp. nov., isolated from farmed Atlantic cod (Gadus morhua L.). Int. J. Syst. Evol. Microbiol., 57(9):1960-1965, http://dx.doi.org/10.1099/ijs.0.64765-0.
Nylund A, Ottem K F, Watanabe K, Karlsbakk E, Krossoy B. 2006. Francisella sp. (Family Francisellaceae) causing mortality in Norwegian cod (Gadus morhua) farming. Arch. Microbiol., 185(5):383-392, http://dx.doi.org/10.1007/s00203-006-0109-5.
Olsen A, Mikalsen J, Rode M, Alfjorden A, Hoel E, Straum-Lie K, Haldorsen R, Colquhoun D. 2006. A novel systemic granulomatous infl ammatory disease in farmed Atlantic cod, Gadus morhua L., associated with a bacterium belonging to the genus Francisella. Journal of Fish Diseases, 29(5):307-311, http://dx.doi.org/10.1111/j.1365-2761.2006.00714.x.
Ostland V E, Stannard J A, Creek J J, Hedrick R P, Ferguson H W, Carlberg J M, Westerman M E. 2006. Aquatic Francisella-like bacterium associated with mortality of intensively cultured hybrid striped bass Morone chrysops×M. saxatilis. Diseases of Aquatic Organisms, 72(2):135-145, http://dx.doi.org/10.3354/dao072135.
Ottem K F, Nylund A, Karlsbakk E, Friis-Moller A, Krossoy B. 2007. Characterization of Francisella sp., GM2212, the first Francisella isolate from marine fish, Atlantic cod(Gadus morhua). Arch. Microbiol., 187(5):343-350, http://dx.doi.org/10.1007/s00203-006-0198-1.
Romana-Eguia M R R, Ikeda M, Basiao Z U, Taniguchi N. 2004. Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA analysis. Aquaculture, 236(1-4):131-150, http://dx.doi.org/10.1016/j.aquaculture.2004.01.026.
Schefzyk M, Richter E, Röhrbein J H, Schaefer T, Wedi B, Raap U. 2012. The fate of 20 sea breams. Mycobacterium marinum infection. Hautarzt., 63(9):716-718, http://dx.doi.org/10.1007/s00105-011-2316-5. (in German)
Soto E, Baumgartner W, Wiles J, Hawke J P. 2011. Francisella asiatica as the causative agent of piscine francisellosis in cultured tilapia (Oreochromis sp.) in the United States. J. Vet. Diagn. Invest., 23(4):821-825, http://dx.doi.org/10.1177/1040638711407058.
Soto E, Hawke J P, Fernandez D, Morales J A. 2009. Francisella sp., an emerging pathogen of tilapia, Oreochromis niloticus(L.), in Costa Rica. J. Fish Dis., 32(8):713-722, http://dx.doi.org/10.1111/j.1365-2761.2009.01070.x.
Varello K, Prearo M, Serracca L, Meloni D, Rossini I, Righetti M, Pezzolato M, Fioravanti M L, Ercolini C, Bozzetta E. 2014. Granulomatous lesions in a wild mullet population from the eastern Ligurian Sea (Italy):mycobacteriosis vs. pseudotuberculosis. J. Fish Dis., 37(6):553-558, http://dx.doi.org/10.1111/jfd.12155.
Vojtech L N, Sanders G E, Conway C, Ostland V, Hansen J D. 2009. Host immune response and acute disease in a zebrafish model of Francisella pathogenesis. Infect. Immun., 77(2):914-925, http://dx.doi.org/10.1128/IAI.01201-08.
Wang G L, Yuan S P, Jin S. 2005. Nocardiosis in large yellow croaker, Larimichthys crocea (Richardson). J. Fish Dis., 28(6):339-345, http://dx.doi.org/10.1111/j.1365-2761.2005.00637.x.
Zerihun M A, Berg V, Lyche J L, Colquhoun D J, Poppe T T. 2011. Mycobacterium salmoniphilum infection in burbot Lota lota. Dis. Aquat. Organ., 95(1):57-64, http://dx.doi.org/10.3354/dao02347.
Zhang J T, Zhou S M, An S W, Chen L, Wang G L. 2014. Visceral granulomas in farmed large yellow croaker, Larimichthys crocea (Richardson), caused by a bacterial pathogen, Pseudomonas plecoglossicida. J. Fish Dis., 37(2):113-121, http://dx.doi.org/10.1111/jfd.12075.