Phylogenetic diversity of culturable bacteria in surface seawater from the Drake Passage, Antarctica -- Journal of Oceanology and Limnology,2016,34(5):952-963

	Cite this paper:
	LI Zhao, XING Mengxin, WANG Wei, WANG Dan, ZHU Jiancheng, SUN Mi. Phylogenetic diversity of culturable bacteria in surface seawater from the Drake Passage, Antarctica[J]. Journal of Oceanology and Limnology, 2016, 34(5): 952-963

Phylogenetic diversity of culturable bacteria in surface seawater from the Drake Passage, Antarctica

LI Zhao, XING Mengxin, WANG Wei, WANG Dan, ZHU Jiancheng, SUN Mi

Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Research Center for Quality and Safety of Marine Biological Resources and Control Engineering in Shandong, Qingdao 266000, China

Abstract:

The Drake Passage is located between the Antarctic Peninsula and Tierra del Fuego in the south of South America. Surface seawater samples were collected at seven sites in the Drake Passage during the austral summer of 2012. The 16S rRNA sequences were analyzed from 187 isolated bacterial strains. Three phyla, 29 genera and 56 species were identified. The three phyla were Actinobacteria, Firmicutes and Proteobacteria; the Proteobacteria included α-Proteobacteria, β-Proteobacteria and γ-Proteobacteria. γ-Proteobacteria, Actinobacteria and Firmicutes were the dominant class or phyla in terms of quantity and species. Gram-positive bacteria (Actinobacteria and Firmicutes) accounted for 57.8% of all types identified. There were nine dominant genera, including Curtobacterium, Staphylococcus, and Halomonas, and 14 dominant species including Curtobacterium flaccumfaciens, Curtobacterium pusillum, and Staphylococcus sciuri. Of the strains identified, 87.2% were catalase positive or weakly positive.

Key words: Antarctic|surface seawater|culturable bacteria|phylogenetic diversity|catalase activities

Received: 2015-04-21 Revised: 2015-06-15

Tools

PDF (607 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by LI Zhao

Articles by XING Mengxin

Articles by WANG Wei

Articles by WANG Dan

Articles by ZHU Jiancheng

Articles by SUN Mi

References:
Abele D, Burlando B, Viarengo A, Pörtner H O. 1998.Exposure to elevated temperatures and hydrogen peroxide elicits oxidative stress and antioxidant response in the Antarctic intertidal limpet Nacella concinna. Comparative Biochemistry and Physiology, 120(2):425-435.
Abele D, Ferreyra G A, Schloss I. 1999. H₂O₂ accumulation from photochemical production and atmospheric wet deposition in Antarctic coastal and off-shore waters of Potter Cove, King George Island, South Shetland Islands.Antarctic Science, 11(2):131-139.
Abele-Oeschger D, Sartoris F J, Pörtner H O. 1996. Effect of elevated hydrogen peroxide levels on aerobic metabolic rate, lactate formation, ATP homeostasis and intracellular pH in the sand shrimp Crangon crangon. Comparative Biochemistry and Physiology, 117C:123-129.
Antony R, Krishnan K P, Laluraj C M, Thamban M, Dhakephalkar P K, Engineer A S, Shivaji S. 2012.Diversity and physiology of culturable bacteria associated with a coastal Antarctic ice core. Microbiological Research, 167(6):372-380.
Bano N, Hollibaugh J T. 2002. Phylogenetic composition of bacterioplankton assemblages from the Arctic Ocean.Applied and Environmental Microbiology, 68(2):505-518.
Boraso A, Williams A J. 1994. Modification of the gating of the cardiac sarcoplasmic reticulum Ca²⁺-release channel by H₂O₂ and dithiothreitol. American Physiological Society, 267(3Pt2):H1010-H1016.
Bowman J P, Rea S M, McCammon S A, McMeekin T A. 2000. Diversity and community structure within anoxic sediment from marine salinity meromictic lakes and a coastal meromictic marine basin, Vestfold Hilds, Eastern Antarctica. Environmental Microbiology, 2(2):227-237.
Brinkmeyer R, Knittel K, Jürgens J, Weyland H, Amann R, Helmke E. 2003. Diversity and structure of bacterial communities in arctic versus antarctic pack ice. Applied and Environmental Microbiology, 69(11):6 610-6 619.
Britschgi T B, Giovannoni S J. 1991. Phylogenetic analysis of a natural marine bacterioplankton population by rRNA gene cloning and sequencing. Applied and Environmental Microbiology, 57(6):1 707-1 713.
Brown M V, Bowman J P. 2001. A molecular phylogenetic survey of sea-ice microbial communities (SIMCO).FEMS Microbiology Ecology, 35(3):267-275.
Cooper W J, Zika R G. 1983. Photochemical formation of hydrogen peroxide in surface and ground waters exposed to sunlight. Science, 220(4598):711-712.
De Souza M J, Nair S, Bharathi P A L, Chandramohan D. 2006. Metal and antibiotic-resistance in psychrotrophic bacteria from Antarctic Marine waters. Ecotoxicology, 15(4):379-384.
Fuhrman J A, McCallum K, Davis A A. 1993. Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific oceans. Applied and Environmental Microbiology, 59(5):1 294-1 302.
Ghiglione J F, Galand P E, Pommier T, Pedrós-Alió C, Maas E W, Bakker K, Bertilson S, Kirchman D L, Lovejoy C, Yager P L, Murray A E. 2012. Pole-to-pole biogeography of surface and deep marine bacterial communities.Proceedings of the National Academy of Sciences of the United States of America, 109(43):17 633-17 638.
Glöckner F O, Zaichikov E, Belkova N, Denissova L, Pernthaler J, Pernthaler A, Amann R. 2000. Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed phylogenetic clusters including an abundant group of Actinobacteria. Applied and Environmental Microbiology, 66(11):5 053-5 065.
Groudieva T, Kambourova M, Yusef H, Royter M, Grote R, Trinks H, Antranikian G. 2004. Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen. Extremophiles, 8(6):475-488.
Gupta P, Agrawal H K, Bandopadhyay R. 2015. Distribution pattern of bacteria in the two geographic poles and Southern Ocean from the reported 16S rDNA sequences.Current Science, 108(10):1 926-1 930.
Hitschke K, Bühler R, Apell H J, Stark G. 1994. Inactivation of the Na, K-ATPase by radiation-induced free radicals Evidence for a radical-chain mechanism. FEBS Letters, 353(3):297-300.
Hollibaugh J T, Bano N, Ducklow H W. 2002. Widespread distribution in polar oceans of 16S rRNA gene sequence with affinity to Nitrosospira-like ammonia-oxidizing bacteria. Applied and Environmental Microbiology, 68(3):1 478-1 484.
Johnson S S, Hebsgaard M B, Christensen T R, Mastepanov M, Nielsen R, Munch K, Brand T, Gilbert M T P, Zuber M T, Bunce M, Rønn R, Gilichinsky D, Froese D, Willerslev E. 2007. Ancient bacteria show evidence of DNA repair.Proceedings of the National Academy of Sciences of the United States of America, 104(36):14 401-14 405.
Jones D P. 1985. The role of oxygen concentration in oxidative stress:hypoxic and hyperoxic models. In:Sifs H eds.Oxidative Stress. Academic Press, London. p.151-195.
Karl D M, Resing J, Tien G, Letelier R. 1993. Palmer LTER:Hydrogen peroxide in the Palmer LTER region:I. An introduction. Antarctic Journal of the United States, 28(5):225-226.
Kim O S, Cho Y J, Lee K, Yoon S H, Kim M, Na H, Park S C, Jeon Y S, Lee J H, Yi H, Won S, Chun J. 2012. Introducing EzTaxon-e:a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62(3):716-721.
Legendre L, Ackley S F, Dieckmann G S, Gulliksen B, Horner R, Hoshiai T, Melnikov I A, Reeburgh W S, Spindler M, Sullivan C W. 1992. Ecology of sea ice biota. Polar Biology, 12(3):429-444.
Liu Z L, Ning X R, Cai Y M, Liu C G, Zhu G H. 2000. Primary productivity and chlorophyll a in the surface water on the route encircling the Antarctica during austral summer of 1999-2000. Chinese Journal of Polar Research, 12(4):235-244. (in Chinese with English abstract)
Liu Z L, Ning X R, Zhu G H, Shi J X. 1993. Size-fractionated biomass and productivity of phytoplankton and particulate organic carbon in the surface on the routine encircling the Antarctica. Antarctic Research (Chinese Edition), 5(4):63-72. (in Chinese with English abstract)
Mense M, Stark G, Apell H J. 1997. Effects of free radicals on partial reactions of the Na, K-ATPase. Journa1 of Membrane Biology, 156(1):63-71.
Moffett J W, Zajiriou O C. 1990. An investigation of hydrogen peroxide chemistry in surface waters of Vineyard Sound with H₂¹⁸O₂ and ¹⁸O₂. Limnology and Oceanography, 35(6):1 221-1 229.
Mullins T D, Britschgi T B, Krest R L, Giovannoni S J. 1995.Genetic comparisons reveal the same unknown bacterial lineages in Atlantic and Pacific bacterioplankton communities. Limnology and Oceanography, 40(1):148-158.
Neužil J, Gebicki J M, Stocker R. 1993. Radical-induced chain oxidation of proteins and its inhibition by chain-breaking antioxidants. Biochemical Journal, 293(3):601-606.
Oliveros J C. 2007. VENNY. An interactive tool for comparing lists with Venn Diagrams. http://bioinfogp.cnb.csic.es/tools/venny/index.html.
Prabagaran S R, Manorama R, Delille D, Shivaji S. 2007.Predominance of Roseobacter, Sulfitobacter, Glaciecola and Psychrobacter in seawater collected off Ushuaia, Argentina, Sub-Antarctica. FEMS Microbiology Ecology, 59(2):342-355.
Ravenschlag K, Sahm K, Amann R. 2001. Quantitative molecular analysis of the microbial community in marine Arctic sediments (Svalbard). Applied and Environmental Microbiology, 67(1):387-395.
Riemann L, Leitet C, Pommier T, Simu K, Holmfeldt K, Larsson U, Hagström Å. 2008. The native bacterioplankton community in the central Baltic Sea is infuenced by freshwater bacterial species. Applied and Environmental Microbiology, 74(2):503-515.
Schulte-Frohlinde D, Sonntag C V. 1985. Radiolysis of DNA and model systems in the presence of oxygen. In:Sies H eds. Oxidative Stress. Academic Press, London. p.11-40.
Seiburth J M. 1979. Sea Microbial Seascapes. University Park Press, Baltimore, America.
Sievert S M, Brinkhoff T, Muyzer G, Ziebis W, Kuever J. 1999.Spatial heterogeneity of bacterial populations along an environmental gradient at a shallow submarine hydrothermal vent near milos island (Greece). Applied and Environmental Microbiology, 65(9):3 834-3 842.
Stark G. 1991. The effect of ionizing radiation on lipid membranes. Biochimica et Biophysica Acta (BBA)-Reviews on Biomembranes, 1071(2):103-122.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4:molecular evolutionary genetics analysis (MEGA)software version 4.0. Molecular Biology and Evolution, 24(8):1 596-1 599.
Thomas C E, Reed D J. 1990. Radical-induced inactivation of kidney Na⁺, K⁺,-ATPase:sensitivity to membrane lipid peroxidation and the protective effect of vitamin E.Archives of Biochemistry and Biophysics, 281(1):96-105.
Tindall B J, Sikorski J, Smibert R A, Krieg N R. 2007.Phenotypic characterization and the principles of comparative systematics. In:Reddy C A, Beveridge T J, Breznak J A, Marzluf G, Schmidt T M, Snyder L R eds.Methods for General and Molecular Microbiology. 3rd edn. ASM Press, Washington DC. p.330-393.
Viarengo A, Abele-Oeschgder D, Burlando B. 1998. Effects of low temperature on prooxidants processes and antioxidant defence systems in marine organisms. In:Pörtener H O, Playle R C eds. Cold Ocean Physiology. Cambridge University Press, Cambridge. p.213-235.
Ward A C, Bora N. 2006. Diversity and biogeography of marine actinobacteria. Current Opinion in Microbiology, 9(3):279-286.
Webster N C, Wilson K J, Blackall L L, Hill R T. 2001.Phylogenetic diversity of bacteria associated with the marine sponge Rhopaloeides odorabile. Applied and Environmental Microbiology, 67(1):434-444.
Weisburg W G, Barns S M, Pelletier D A, Lane D J. 1991. 16S ribosomal DNA amplification for phylogenetic study.Journal of Bacteriology, 173(2):697-703.
Xu H S, Roberts N, Singleton F L, Attwell R W, Grimes D J, Colwell R R. 1982. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment. Microbial Ecology, 8(3):313-323.
Yu Y, Li H R, Chen B, Zeng Y X, He J F, Cai M H. 2006.Phylogenetic diversity and cold-adaptive hydrolytic enzymes of culturable psychrophilic bacteria associated with sea ice from high latitude ocean, Arctic. Acta Microbiologica Sinica, 46(2):184-190. (in Chinese with English abstract)
Zdanowski M K, Żmuda-Baranowska M J, Borsuk P, Światecki A, Górniak D, Wolicka D, Jankowska K M, Grzesiak J. 2013. Culturable bacteria community development in postglacial soils of Ecology Glacier, King George Island, Antarctica. Polar Biology, 36(4):511-527.
Zeng Y X, Yu Y, Qiao Z Y, Jin H Y, Li H R. 2014. Diversity of bacterioplankton in coastal seawaters of Fildes Peninsula, King George Island, Antarctica. Archives of Microbiology, 196(2):137-147.
Zeng Y X, Zheng T L, Li H R. 2009. Community composition of the marine bacterioplankton in Kongsfjorden(Spitsbergen) as revealed by 16S rRNA gene analysis.Polar Biology, 32(10):1 447-1 460.
Zeng Y X, Zou Y, Grebmeier J M, He J F, Zheng T L. 2012.Culture-independent and-dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea. Polar Biology, 35(1):117-129.
Zhang X H. 2007. Marine Microbiology. China Ocean University Press, Qingdao, China. (in Chinese)
Zwart G, Hiorns W D, Methé B A, van Agterveld M P, Huismans R, Nold S C, Zehr J P, Lannbroek H J. 1998.Nearly identical 16S rRNA sequences recovered from lakes in North America and Europe indicate the existence of clades of globally distributed freshwater bacteria.Systematic and Applied Microbiology, 21(4):546-556.