Effects of dietary genistein on GH/IGF-I axis of Nile tilapia <i>Oreochromis niloticus</i> -- Journal of Oceanology and Limnology,2016,34(5):1004-1012

	Cite this paper:
	CHEN Dong, WANG Wei, RU Shaoguo. Effects of dietary genistein on GH/IGF-I axis of Nile tilapia Oreochromis niloticus[J]. Journal of Oceanology and Limnology, 2016, 34(5): 1004-1012

Effects of dietary genistein on GH/IGF-I axis of Nile tilapia Oreochromis niloticus

CHEN Dong^1,2, WANG Wei¹, RU Shaoguo¹

1 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;
2 School of Environmental and Municipal Engineering, Qingdao Technological University, Qingdao 266033, China

Abstract:

There is considerable concern that isoflavones, such as genistein in fish feed composed of soybean protein, affects somatic growth in fish. Our previous works demonstrated that 30 and 300 μg/g dietary genistein had no significant effect on growth performance in Nile tilapia (Oreochromis niloticus), but the higher level of genistein (3 000 μg/g) significantly depressed growth. This study was conducted to further examine the effects of dietary genistein on the endocrine disruption on growth hormone/insulin-like growth factor-I (GH/IGF-I) axis in Nile tilapia (O. niloticus). Juvenile fish were fed by hand twice daily to satiation with one of four isonitrogenous and isoenergetic diets, each containing either 0,30, 300 or 3 000 μg/g genistein. Following an 8-week feeding period, plasma GH and IGF-I levels were investigated by radioimmunoassay and gene expression levels of gh, ghrelin, gnrhs, ghr, npy, npyrs, pacap, ghrs, igf-I, igf-Ir, and igfbp3 were examined by real-time PCR. The results show that no significant change in plasma GH and IGF-I levels in fish fed with diets containing 30 μg/g and 300 μg/g genistein. mRNA expression of genes along the GH/IGF-I axis remained unaffected, except for igf-Ir, which was stimulated by the 300 μg/g genistein diet. While in fish fed the 3 000 μg/ggenistein diet, the plasma GH and IGF-I levels decreased, and mRNA expression of gh, ghr2, npyr1, igf-I, and igf-Ir were also significantly depressed. In contrast, npy and igfbp3 mRNA expression were enhanced. This study provides convincing evidence for growth impediment by genistein by disturbing the GH/IGF-I axis in Nile tilapia O. niloticus.

Key words: genistein|hormone/insulin-like growth factor-I (GH/IGF-I) axis|Nile tilapia|growth rate|aquaculture

Received: 2015-01-10 Revised: 2015-04-30

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Articles by WANG Wei

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