Ontogenetic changes in digestive enzyme activities and the amino acid profile of starry flounder <i>Platichthys stellatus</i> -- Journal of Oceanology and Limnology,2016,34(5):1013-1024

	Cite this paper:
	SONG Zhidong, WANG Jiying, QIAO Hongjin, LI Peiyu, ZHANG Limin, XIA Bin. Ontogenetic changes in digestive enzyme activities and the amino acid profile of starry flounder Platichthys stellatus[J]. Journal of Oceanology and Limnology, 2016, 34(5): 1013-1024

Ontogenetic changes in digestive enzyme activities and the amino acid profile of starry flounder Platichthys stellatus

SONG Zhidong¹, WANG Jiying¹, QIAO Hongjin¹, LI Peiyu^1,2, ZHANG Limin¹, XIA Bin¹

1 Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
2 Shandong Shengsuo Aquatic Feed Research Center, Yantai 265601, China

Abstract:

Ontogenetic changes in digestive enzyme activities and the amino acid (AA) profile of starry flounder, Platichthys stellatus, were investigated and limiting amino acids were estimated compared with the essential AA profile between larvae and live food to clarify starry flounder larval nutritional requirements. Larvae were collected at the egg stage and 0, 2, 4, 7, 12, 17, 24 days after hatching (DAH) for analysis. Larvae grew from 1.91 mm at hatching to 12.13 mm at 24 DAH. Trypsin and chymotrypsin activities changed slightly by 4 DAH and then increased significantly 4 DAH. Pepsin activity increased sharply beginning 17 DAH. Lipase activity increased significantly 4 DAH and increased progressively with larval growth. Amylase activity was also detected in newly hatched larvae and increased 7 DAH followed by a gradual decrease. High free amino acid (FAA) content was detected in starry flounder eggs (110.72 mg/g dry weight). Total FAA content dropped to 43.29 mg/g in 4-DAH larvae and then decreased gradually to 13.74 mg/g in 24-DAH larvae. Most FAAs (except lysine and methionine) decreased >50% in 4-DAH larvae compared with those in eggs and then decreased to the lowest values in 24-DAH larvae. Changes in the protein amino acid (PAA) profile were much milder than those observed for FAAs. Most PAAs increased gradually during larval development, except lysine and phenylalanine. The percentages of free threonine, valine, isoleucine, and leucine decreased until the end of the trial, whereas the protein forms of these four AAs followed the opposite trend. A comparison of the essential AA composition of live food (rotifers, Artemia nauplii, and Artemia metanauplii) and larvae suggested that methionine was potentially the first limiting AA. These results may help develop starry flounder larviculture methods by solving the AA imbalance in live food. Moreover, the increased digestive enzyme activities indicate the possibility of introducing artificial compound feed.

Key words: aquaculture|flatfish|larval development|weaning food|limiting amino acid

Received: 2015-03-10 Revised: 2015-04-20

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Articles by SONG Zhidong

Articles by WANG Jiying

Articles by QIAO Hongjin

Articles by LI Peiyu

Articles by ZHANG Limin

Articles by XIA Bin

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