Activation of macrophages by an exopolysaccharide isolated from Antarctic <i>Psychrobacter</i> sp. B-3 -- Journal of Oceanology and Limnology,2016,34(5):1064-1071

	Cite this paper:
	YU Leiye, SUN Guojie, WEI Jingfang, WANG Yingze, DU Chao, LI Jiang. Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3[J]. Journal of Oceanology and Limnology, 2016, 34(5): 1064-1071

Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

YU Leiye¹, SUN Guojie¹, WEI Jingfang¹, WANG Yingze¹, DU Chao¹, LI Jiang²

1 College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China;
2 Key Laboratory of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration(SOA), Qingdao 266061, China

Abstract:

An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF)α secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and IκBα degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-κB signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.

Key words: exopolysaccharide (EPS)|Antarctic bacterium|RAW264.7|TLR4|NF-κB

Received: 2014-12-25 Revised: 2015-06-06

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Articles by YU Leiye

Articles by SUN Guojie

Articles by WEI Jingfang

Articles by WANG Yingze

Articles by DU Chao

Articles by LI Jiang

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