Biomass and nutrient productivities of <i>Tetraselmis chuii</i> under mixotrophic culture conditions with various C: N ratios -- Journal of Oceanology and Limnology,2017,35(2):303-312

	Cite this paper:
	LU Lin, WANG Jun, YANG Guanpin, ZHU Baohua, PAN Kehou. Biomass and nutrient productivities of Tetraselmis chuii under mixotrophic culture conditions with various C: N ratios[J]. Journal of Oceanology and Limnology, 2017, 35(2): 303-312

Biomass and nutrient productivities of Tetraselmis chuii under mixotrophic culture conditions with various C: N ratios

LU Lin¹, WANG Jun², YANG Guanpin², ZHU Baohua¹, PAN Kehou^1,3

1 Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China;
2 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;
3 Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

Abstract:

Mass microalgal culture plays an irreplaceable role in aquaculture, but microalgal productivity is restricted by traditional autotrophic culture conditions. In the present study, a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea. The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen (C:N) ratios than those under autotrophic conditions. When the C:N ratio was 16, the optical density and biomass productivity were 3.7- and 5-fold higher than their corresponding values under autotrophic culture conditions, respectively. Moreover, T. chuii synthesized more polysaccharides and total lipids under mixotrophic conditions. In addition, T. chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions. At a C:N ratio of 16, the percentage of C16:0 and C18:1 reached 30.08% and 24.65% of the total fatty acid (TFA) content, respectively. These findings may provide a basis for largescale mixotrophic culture of T. chuii, as a potential bait-microalga.

Key words: bait-microalga|C:N ratio|mixotrophic culture|Tetraselmis chuii

Received: 2015-10-23 Revised: 2015-12-31

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