Measurement and feature analysis of absorption spectra of four algal species -- Journal of Oceanology and Limnology,2017,35(2):350-359

	Cite this paper:
	ZHU Jianhua, ZHOU Hongli, HAN Bing, LI Tongji. Measurement and feature analysis of absorption spectra of four algal species[J]. Journal of Oceanology and Limnology, 2017, 35(2): 350-359

Measurement and feature analysis of absorption spectra of four algal species

ZHU Jianhua, ZHOU Hongli, HAN Bing, LI Tongji

National Ocean Technology Center, Tianjin 300111, China

Abstract:

Two methods for particulate pigments (i.e., quantitative filter technique, QFT, and in vivo measurement, InVivo, respectively) and two methods for dissolved pigments (i.e., Acetone Extracts, AceEx, and high-performance liquid chromatography, HPLC, respectively) were used to obtain the optical absorption coefficient spectra for cultures of four typical algal species. Through normalization and analysis of the spectra, it is shown that (1) the four methods are able to measure optical absorption spectra of particulate and/or dissolved pigments; (2) that the optical absorption spectra of particulate and dissolved pigments were consistent in terms of the peak position in the blue wavelength, and the difference of the peak position in the near infrared wavelength was~10 nm between each other; and (3) that the leveling effect of the absorption spectra of particulate pigments was significant. These four methods can all effectively measure the absorption coefficients of phytoplankton pigments, while each one has its unique advantages in different applications. Therefore, appropriate method should be carefully selected for various application due to their intrinsic difference.

Key words: algal species|optical absorption coefficient|spectral pattern|normalization

Received: 2015-11-12 Revised: 2015-12-31

Tools

PDF (574 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by ZHU Jianhua

Articles by ZHOU Hongli

Articles by HAN Bing

Articles by LI Tongji

References:
Abramowitz A, Segun I A. 1968. Handbook of Mathematical Functions. Dover, New York.
Akima H. 1970. A new method of interpolation and smooth curve fitting based on local procedures. Journal of the ACM, 17(4):589-602.
Allali K, Bricaud A, Babin M, Morel A, Chang P. 1995. A new method for measuring spectral absorption coefficients of marine particles. Limnology and Oceanography, 40(8):1 526-1 523.
Bidigare R R, Morrow J H, Kiefer D A. 1989. Derivative analysis of spectral absorption by photosynthetic pigments in the western Sargasso Sea. Journal of Marine Research, 47(2):323-341.
Bidigare R R, Ondrusek M E, Morrow J H et al. 1990. In-vivo absorption properties of algal pigments. In:Proceedings of SPIE, Ocean Optical X. SPIE, Orlando, FL, United States, 1302:290-302.
Bricaud A, Babin M, Morel A et al. 1995. Variability in the chlorophyll-specific absorption coefficients of natural phytoplankton analysis and parameterize:analysis and parameterization. Journal of Geophysical Research, 100(C7):13 321-13 332.
Bricaud A, Claustre H, Ras J et al. 2004. Natural variability of phytoplanktonic absorption in oceanic waters:influence of the size structure of algal populations. Journal of Geophysical Research Oceans, 109(C11), http://dx.doi.org/10.1029/2004JC002419.
Bricaud A, Mejia C, Blondeau-Patissier D et al. 2007. Retrieval of pigment concentrations and size structure of algal populations from their absorption spectra using multilayered perceptrons. Appl. Opt., 46(8):1 251-1 260.
Bricaud A, Stramski D. 1995. Spectral absorption coefficients of living phytoplankton and non-algal biogenous matter:a comparison between the Peru upwelling area and the Sargasso Sea. Limnology and Oceanography, 35(3):562-582.
Chavez F, Buck K R, Bidigare R R, Karl D M, Hebel D, Latasa M, Campbell L, Newton J. 1995. On the chlorophyll a retention properties of glass-fiber GF/F filters. Limnology and Oceanography, 40(2):428-433.
Cleveland J S. 1995. Regional models for phytoplankton absorption as a function of chlorophyll a concentration.Journal of Geophysical Research, 100(C7):13 333-13 344.
Ferrari G M, Tassan S. 1996. Use of the 0.22 μm Millipore membrane for light-transmission measurements of aquatic particles. Journal of Plankton Research, 18(7):1 261-1 267.
Ferrari G M, Tassan S. 1999. A method using chemical oxidation to remove light absorption by phytoplankton pigments. Journal of Phycology, 35(5):1 090-1 098.
Finkel Z V. 2001. Light absorption and size scaling of lightlimited metabolism in marine Diatoms. Limnology and Oceanography, 46(1):86-94.
Jeffrey S W, Vesk M. 1997. Introduction to marine phytoplankton and their pigment signatures. In:Jeffrey S W, Mantoura R F C, Wright S W eds. Phytoplankton Pigments in Oceanography:Guidelines to Modern Methods. UNESCO, Paris, France.
Lan W L, Li M M, Li T S. 2013. Phytoplankton community structure based on pigment composition in Qinzhou bay during average water period. Acta Ecologica Sinica,33(20):6 595-6 603. (in Chinese with English abstract)
Lohrenz S E, Weidemann A D, Tuel M. 2003. Phytoplankton spectral absorption as influenced by community size structure and pigment composition. Journal of Plankton Research, 25(1):35-61.
Lorenzen C J 1967. Determination of chlorophyll and pheopigments:spectrophotometric equations. Limnology and Oceanography, 12(2):343-346.
Marra J, Trees C C, O'Reilly J E. 2007. Phytoplankton pigment absorption:a strong predictor of primary productivity in the surface ocean. Deep Sea Research Part I:Oceanographic Research Papers, 54(2):155-163.
Maske H, Haardt H. 1987. Quantitative in vivo absorption spectra of phytoplankton:detrital absorption and comparison with fluorescence excitation spectra.Limnology and Oceanography, 32(3):620-633.
Millán-Núñez E, Lara-Lara J R, Cleveland J S et al. 1998.
Variations in specific absorption coefficients and total phytoplankton in the gulf of california. California Cooperative Oceanic Fisheries Investigations Reports, 39(10):159-168.
Mitchell B G, Kiefer D A. 1988a. Chlorophyll a specific absorption and fluorescence excitation spectra for lightlimited phytoplankton. Deep Sea Research Part A.Oceanographic Research Papers, 35(5):639-663.
Mitchell B G. 1990. Algorithms for determining the absorption coefficient of aquatic particulates using the quantitative filter technique. In:Proceedings of SPIE, Ocean Optics X.SPIE, Orlando, FL, United States. 1302:137-148.
Mitchell G, Bricaud A, Carder K et al. 2000. Determination of spectral absorption coefficients of particles, dissolved material, and phytoplankton for discrete water samples.In:Fargion G S, Mueller J L eds. Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 2.NASA/TM-2000-209966, NASA, Goddard Space Flight Center, Greenbelt, MD. p.125-153.
Morel A, Bricaud A. 1981. Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton. Deep Sea Research Part A. Oceanographic Research Papers, 28(11):1 375-1 393.
Ras J, Claustre H, Uitz J. 2008. Spatial variability of phytoplankton pigment distributions in the Subtropical South Pacific Ocean:comparison between in situ and predicted data. Biogeosciences, 5(2):353-369.
Ruivo M, Amorim A, Cartaxana P. 2011. Effects of growth phase and irradiance on phytoplankton pigment ratios:implications for chemotaxonomy in coastal waters.Journal of Plankton Research, 33(7):1 012-1 022.
Sathyendranath S, Lazzara L, Prieur L. 1987. Variations in the spectral values of specific absorption of phytoplankton. Limnology and Oceanography, 32(2):403-415.
Suzuki K, Kishino M, Sasaoka K, Saitoh S I, Saino T. 1998.Chlorophyll-specific absorption coefficients and pigments of phytoplankton off Sanriku, northwestern north Pacific.Journal of Oceanography, 54(5):517-526.
Tassan S, Ferrari G M, Bricaud A, Babin M. 2000. Variability of the amplification factor of light absorption by filterretained aquatic particles in the coastal environment.Journal of Plankton Research, 22(4):659-668.
Tassan S, Ferrari G M. 1995a. An alternative approach to absorption measurements of aquatic particles retained on filters. Limnology and Oceanography, 40(8):1 358-1 368.
Tassan S, Ferrari G. 1998. Measurement of the light absorption by aquatic particles retained on filters:determination of the optical pathlength amplification by the ‘transmittancereflectance’ method. Journal of Plankton Research, 20(9):1 699-1 709.
van de Hulst H C. 1957. Light Scattering by Small Particles.John Wiley & Sons, New York, US.
Wang G F, Cao W X, Xu D Z et al. 2007. Effects of size structure and pigment composition of algal population on phytoplankton absorption coefficients in the South China Sea. Acta Oceanologica Sinica, 29(1):38-48.
Wright S W, Jeffrey S W, Mantoura R F C, Llewellyn C A, Bjørnland T, Repeta D, Welschmeyer N. 1991. Improved HPLC method for the analysis of chlorophylls and carotenoids from marine phytoplankton. Mar. Ecol. Prog.Ser., 77(2-3):183-196.
Yentsch C S, Phinney D A. 1989. A bridge between ocean optics and microbial ecology. Limnology and Oceanography, 34(8):1 694-1 705.
Yentsch C S. 1960. The influence of phytoplankton pigments on the colour of sea water. Deep Sea Research, 7(1):1-9.
Yentsch C S. 1962. Measurement of visible light absorption by particulate matter in the ocean. Limnology and Oceanography, 7(2):207-217.
Zhou H L, Zhu J H, Han B et al. 2010. Comparison of spectral absorption characteristics of several typical algae. Journal of Ocean Technology, 29(3):78-81, 92. (in Chinese with English abstract)
Zhou H L, Zhu J H, Yang A A et al. 2011. Analysis of prorocentrum donghaiense lu absorb spectral characteristic. Journal of Ocean Technology, 30(1):65-67, 77. (in Chinese with English abstract)
Zhu J H, Li T J. 2004. Relationship research of phytoplankton pigments absorption and chlorophyll a concentration in in the East China Sea and Yellow Sea. Journal of Ocean Technology, 23(4):117-122. (in Chinese with English abstract)
Zhu J H, Wu H M, Zhou H L et al. 2005. Phytoplankton pigments concentration of Qinghai Lake by HPLC.Journal of Ocean Technology, 24(2):46-49, 54. (in Chinese with English abstract)
Zhu J H. 2003. Measurement of the ocean phytoplankton pigments concentration by HPLC. Journal of Ocean Technology, 22(1):14-19. (in Chinese with English abstract)
Zhuang Y P, Jin H Y, Li H L et al. 2014. Phytoplankton composition and its ecological effect in subsurface cold pool of the northern Bering Sea in summer as revealed by HPLC derived pigment signatures. Acta Oceanologica Sinica, 33(6):103-111.