Spatiotemporal variation of dissolved carbohydrates and amino acids in Jiaozhou Bay, China -- Journal of Oceanology and Limnology,2017,35(2):383-399

	Cite this paper:
	SHI Di, YANG Guipeng, SUN Yan, WU Guanwei. Spatiotemporal variation of dissolved carbohydrates and amino acids in Jiaozhou Bay, China[J]. Journal of Oceanology and Limnology, 2017, 35(2): 383-399

Spatiotemporal variation of dissolved carbohydrates and amino acids in Jiaozhou Bay, China

SHI Di¹, YANG Guipeng^1,2,3, SUN Yan¹, WU Guanwei^1,3

1 Key Laboratory of Marine Chemistry Theory and Technology, Ocean University of China, Ministry of Education/Qingdao Collaborative Innovation Center of Marine Science and Technology, Qingdao 266100, China;
2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 Institute of Marine Chemistry, Ocean University of China, Qingdao 266100, China

Abstract:

Surface seawater samples were collected from Jiaozhou Bay, China, during six cruises (March- May 2010, September-November 2010) to study the distribution of dissolved organic matter including dissolved organic carbon (DOC), total dissolved carbohydrates, namely monosaccharides (MCHO) and polysaccharides (PCHO) and total hydrolysable amino acids. These included dissolved free amino acids (DFAA) and combined amino acids (DCAA). The goal was to investigate possible relationships between these dissolved organic compounds and environmental parameters. During spring, the concentrations of MCHO and PCHO were 9.6 (2.8-22.6) and 11.0 (2.9-42.5) μmol C/L, respectively. In autumn, MCHO and PCHO were 9.1 (2.6-27.0) and 10.8 (2.4-25.6) μmol C/L, respectively. The spring concentrations of DFAA and DCAA were 1.7 (1.1-4.1) and 7.6 (1.1-31.0) μmol C/L, respectively, while in autumn, DFAA and DCAA were 2.3 (1.1-8.0) and 3.3 (0.6-7.2) μmol C/L, respectively. Among these compounds, the concentrations of PCHO were the highest, accounting for nearly a quarter of the DOC, followed by MCHO, DCAA and DFAA. The concentrations of the organic compounds exhibited a decreasing trend from the coastal to the central regions of the bay. A negative correlation between concentrations of DOC and salinity in each cruise suggested that riverine inputs around the bay have an important impact on the distribution of DOC in the surface water. A significant positive correlation was found between DOC and total bacteria count in spring and autumn, suggesting bacteria play an important role in the marine carbon cycle.

Received: 2015-08-09 Revised: 2015-10-26

Tools

PDF (854 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by SHI Di

Articles by YANG Guipeng

Articles by SUN Yan

Articles by WU Guanwei

References:
Admiraal W, Riaux-Gobin C, Laane R W P M. 1987.Interactions of ammonium, nitrate, and D- and L-amino acids in the nitrogen assimilation of two species of estuarine benthic diatoms. Mar. Ecol. Prog. Ser., 40(3):267-273.
Amon R M W, Benner R. 2003. Combined neutral sugars as indicators of the diagenetic state of dissolved organic matter in the Arctic Ocean. Deep Sea Res. Part I, 50(1):151-169.
Amon R M W, Fitznar H P, Benner R. 2001. Linkages among the bioreactivity, chemical composition, and diagenetic state of marine dissolved organic matter. Limnol.Oceanogr., 46(2):287-297.
Avigad G. 1968. A modified procedure for the colorimetric, ultramicro determination of reducing sugars with the alkaline ferricyanide reagent. Carbohydr. Res., 7:94-97.
Azam F, Fenchel T, Field J G, Gray J S, Meyer-Reil L A, Thingstad F. 1983. The ecological role of water-column microbes in the sea. Mar. Ecol. Prog. Ser., 10:257-263.
Bard A, Goldberg E D, Spencer D W. 1988. Modern chemistry and chemical technology applied to the ocean and its resources introduction. Appl. Geochem., 3(1):3-8.
Benner R. 2002. Chemical composition and reactivity. In:Hansell D A, Carlson C A eds. Biogeochemistry of Marine Dissolved Organic Matter. Academic Press, New York.p.59-90.
Bhosle N B, Bhaskar P V, Ramachandran S. 1998. Abundance of dissolved polysaccharides in the oxygen minimum layer of the Northern Indian Ocean. Mar. Chem., 63(1-2):171-182.
Børsheim K Y, Vadstein O, Myklestad S M, Reinertsen H, Kirkvold S, Olsen Y. 2005. Photosynthetic algal production, accumulation and release of phytoplankton storage carbohydrates and bacterial production in a gradient in daily nutrient supply. J. Plankton Res., 27(8):743-755.
Carlson C A, Ducklow H W, Michaels A F. 1994. Annual flux of dissolved organic carbon from the euphotic zone in the northwestern Sargasso Sea. Nature, 371(6496):405-408.
Chen S J, Sun W X, Wang H T. 1982. Numerical modeling of the circulation and the pollutant dispersion in Jiaozhou Bay Ⅱ. Computation of pollutant dispersion. J. Shandong Coll. Oceanol., 12(4):1-12. (in Chinese with English abstract)
Chen Y, Yang G P, Wu G W, Gao X C, Xia Q Y. 2013.Concentration and characterization of dissolved organic matter in the surface microlayer and subsurface water of the Bohai Sea, China. Cont. Shelf Res., 52:97-107.
Chester R. 2000. Marine Geochemistry. 2nd ed. Blackwell Science Ltd, Oxford. 506p.
Garrasi C, Degens E T, Mopper K. 1979. The free amino acid composition of seawater obtained without desalting and preconcentration. Mar. Chem., 8(1):71-85.
Görs S, Rentsch D, Schiewer U, Karsten U, Schumann R. 2007. Dissolved organic matter along the eutrophication gradient of the Darβ-Zingst Bodden Chain, Southern Baltic Sea:I. Chemical characterisation and composition.Mar. Chem., 104(3-4):125-142.
Granum E, Kirkvold S, Myklestad S M. 2002. Cellular and extracellular production of carbohydrates and amino acids by the marine diatom Skeletonema costatum:diel variations and effects of N depletion. Mar. Ecol. Prog.Ser., 242:83-94.
Guéguen C, Guo L D, Wang D L, Tanaka N, Hung C C. 2006.Chemical characteristics and origin of dissolved organic matter in the Yukon River. Biogeochemistry, 77(2):139-155.
Hansell D A, Carlson C A. 1998. Net community production of dissolved organic carbon. Global Biogeochem. Cycles., 12(3):443-453.
Hedges J I. 1992. Global biogeochemical cycles:progress and problems. Mar. Chem., 39(1-3):67-93.
Hernes P J, Hedges J I, Peterson M L, Wakeham S G, Lee C. 1996. Neutral carbohydrate geochemistry of particulate material in the central equatorial Pacific. Deep Sea Res.Part Ⅱ, 43(4-6):1 181-1 204.
Hubberten U, Lara R J, Kattner G. 1994. Amino acid composition of seawater and dissolved humic substances in the Greenland Sea. Mar. Chem., 45(1-2):121-128.
Hung C C, Tang D G, Warnken K W, Santschi P H. 2001.Distributions of carbohydrates, including uronic acids, in estuarine waters of Galveston Bay. Mar. Chem., 73(3-4):305-318.
Ietswaart T, Schneider P J, Prins R A. 1994. Utilization of organic nitrogen sources by two phytoplankton species and a bacterial isolate in a pure and a mixed cultures.Appl. Environ. Microbiol., 60(5):1 554-1 560.
Ji M H, Cao W D, Qiu X Q. 1983. Distribution of dissolved carbohydrate in seawater of Jiaozhou Bay. Trans.Oceanol. Limnol., (2):40-44. (in Chinese with English abstract)
Keil R G, Kirchman D L. 1991. Dissolved combined amino acids in marine waters as determined by a vapor-phase hydrolysis method. Mar. Chem., 33(3):243-259.
Keil R G, Kirchman D L. 1993. Dissolved combined amino acids:chemical form and utilization by marine bacteria.Limnol. Oceanogr., 38(6):1 256-1 270.
Khodse V B, Bhosle N B, Matondkar S G P. 2010. Distribution of dissolved carbohydrates and uronic acids in a tropical estuary, India. J. Earth Syst. Sci., 119(4):519-530.
Khodse V B, Bhosle N B. 2011. Bacterial utilization of sizefractionated dissolved organic matter. Aquat. Microb.Ecol., 64(3):299-309.
Khodse V B, Fernandes L, Bhosle N B, Sardessai S. 2008.Carbohydrates, uronic acids and alkali extractable carbohydrates in contrasting marine and estuarine sediments:distribution, size fractionation and partial chemical characterization. Org. Geochem., 39(3):265-283.
Linares A, Sundbäck K. 2006. Uptake of dissolved free amino acids (DFAA) by microphytobenthic communities. Aquat.Microb. Ecol., 42(2):175-186.
Lindroth P, Mopper K. 1979. High performance liquid chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde. Anal. Chem., 51(11):1 667-1 674.
Liu D Y, Sun J, Zhang L Y. 2003. Structural characteristics of phytoplankton community during harmful algae bloom in Jiaozhou Bay. Chin. J. Appl. Ecol., 14(11):1 963-1 966.(in Chinese with English abstract)
Liu S M, Zhang J, Chen H T, Zhang G S. 2005. Factors influencing nutrient dynamics in the eutrophic Jiaozhou Bay, North China. Prog. Oceanogr., 66(1):66-85.
Meon B, Kirchman D L. 2001. Dynamics and molecular composition of dissolved organic material during experimental phytoplankton blooms. Mar. Chem., 75(3):185-199.
Myklestad S M, Børsheim K Y. 2007. Dynamics of carbohydrates in the Norwegian Sea inferred from monthly profiles collected during 3 years at 66°N, 2°E.Mar. Chem., 107(4):475-485.
Myklestad S M, Skånøy E, Hestmann S. 1997. A sensitive and rapid method for analysis of dissolved mono- and polysaccharides in seawater. Mar. Chem., 56(3-4):279-286.
Pakulski J D, Benner R. 1994. Abundance and distribution of carbohydrates in the ocean. Limnol. Oceanogr., 39(4):930-940.
Parsons T R, Maita Y, Lalli C M. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford. p.23-58.
Pettine M, Patrolecco L, Manganelli M, Capri S, Farrace M G. 1999. Seasonal variations of dissolved organic matter in the northern Adriatic Sea. Mar. Chem., 64(3):153-169.
Pomeroy L R. 1974. The ocean's food web, a changing paradigm. BioScience, 24(9):499-504.
Porter K G, Feig Y S. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25(5):943-948.
Rawat I, Ranjith R R, Mutanda T, Bux F. 2011. Dual role of microalgae:phycoremediation of domestic wastewater and biomass production for sustainable biofuels production. Appl. Energy, 88(10):3 411-3 424.
Senior W, Chevolot L. 1991. Studies of dissolved carbohydrates(or carbohydrate-like substances) in an estuarine environment. Mar. Chem., 32(1):19-35.
Shen Z L. 2001. Historical changes in nutrient structure and its influences on phytoplantkon composition in Jiaozhou Bay. Estuar. Coast. Shelf Sci., 52(2):211-224.
Shiah F K, Gong G C, Chen T Y, Chen C C. 2000. Temperature dependence of bacterial specific growth rates on the continental shelf of the East China Sea and its potential application in estimating bacterial production. Aquat.Microb. Ecol., 22(2):155-162.
Skoog A, Benner R. 1997. Aldoses in various size fractions of marine organic matter:Implications for carbon cycling.Limnol. Oceanogr., 42(8):1 803-1 813.
Sommerville K, Preston T. 2001. Characterisation of dissolved combined amino acids in marine waters. Rapid Commun.Mass Spectrom., 15(15):1 287-1 290.
Søndergaard M, Middelboe M. 1995. A cross-system analysis of labile dissolved organic carbon. Mar. Ecol. Prog. Ser., 118:283-294.
Sugimura Y, Suzuki Y. 1983. Amino acids dissolved in the western North Pacific waters. Papers Meteorol. Geophys., 34(4):267-289.
Sun S, Sun X X, Zhang G T, Tang H B, Liu Q, Li G M. 2011.Long-term changes in major meteorological and hydrological factors in the Jiaozhou Bay. Oceanol.Limnol. Sinica, 42(5):632-638. (in Chinese with English abstract)
Wang X R, Cai Y H, Guo L D. 2010. Preferential removal of dissolved carbohydrates during estuarine mixing in the Bay of Saint Louis in the northern Gulf of Mexico. Mar.Chem., 119(1-4):130-138.
Witter A E, Luther Ⅲ G W. 2002. Spectrophotometric measurement of seawater carbohydrate concentrations in neritic and oceanic waters from the U.S. Middle Atlantic Bight and the Delaware estuary. Mar. Chem., 77(2-3):143-156.
Wu G W, Yang G P. 2013. Distributions of dissolved carbohydrates in the Yellow Sea and the Northern East China Sea in early winter. J. Coast. Res., 29(2):449-459.
Yamashita Y, Tanoue E. 2003. Distribution and alteration of amino acids in bulk DOM along a transect from bay to oceanic waters. Mar. Chem., 82(3-4):145-160.
Yang G P, Zhang Y P, Lu X L, Ding H B. 2010. Distributions and seasonal variations of dissolved carbohydrates in the Jiaozhou Bay, China. Estuar. Coast. Shelf Sci., 88(1):12-20.
Yao Y, Zheng S Q, Shen Z L. 2007. Study on the mechanism of eutrophication in the Jiaozhou Bay. Mar. Sci. Bull., 26(4):91-98. (in Chinese with English abstract)
Youssef D H, El-Said G F, Shobier A H. 2014. Distribution of total carbohydrates in surface sediments of the Egyptian Mediterranean coast, in relation to some inorganic factors.Arab. J. Chem., 7(5):823-832.
Zhang G L, Zhang J, Xu J, Zhang F. 2006. Distributions, sources and atmospheric fluxes of nitrous oxide in Jiaozhou Bay. Estuar. Coast. Shelf Sci., 68(3-4):557-566.
Zhang Y P, Yang G P, Chen Y. 2009. Chemical characterization and composition of dissolved organic matter in Jiaozhou Bay. Chin. J. Oceanol. Limnol., 27(4):851-858.
Zhang Z B. 2004. Marine Chemistry. China Ocean University Press, Qingdao, China. p.188. (in Chinese)
Zhou C Y, Zhang G L, Yang Y P, Chen C M. 1996. The characterization and the distribution of the dissolved free amino acids in Jiulong River Estuary, Xiamen. J. Xiamen Univ. (Nat. Sci.), 35(3):402-406. (in Chinese with English abstract)