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WANG Hui, NIU Jingyan, LIU Jiahui, YANG Hongshuai, LIU Zhigang. Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach[J]. Journal of Oceanology and Limnology, 2017, 35(3): 537-545

Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

WANG Hui1, NIU Jingyan2, LIU Jiahui3, YANG Hongshuai3, LIU Zhigang3
1 Life Sciences College, Huaiyin Normal University, Huai'an 223300, China;
2 Polytechnic Department, Jiaozuo Teachers College, Jiaozuo 454000, China;
3 Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China
The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate (SGR) and three important environmental factors (temperature, salinity, and pH) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant (P < 0.05), and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant (P > 0.05); the interactive effect of temperature and pH was significant (P < 0.05), whereas the temperature × salinity and salinity × pH interactions were not significant (P > 0.05); all of the quadratic effects of the three factors were significant (P < 0.05). A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25℃/35 of salinity/pH 7.9 was obtained, at which the maximum specific growth rate (0.65) was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.
Key words:    Tetraselmis subcordiformis|specific growth|temperature|salinity|pH|growth model   
Received: 2015-10-24   Revised: 2016-04-05
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