Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber <i>Apostichopus japonicus</i> -- Journal of Oceanology and Limnology,2015,33(2):309-318

	Cite this paper:
	LI Chengze, CHANG Yaqing, PANG Zhenguo, DING Jun, JI Nanjing. Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus[J]. Journal of Oceanology and Limnology, 2015, 33(2): 309-318

Expression responses of five cold tolerant related genes to two temperature dropping treatments in sea cucumber Apostichopus japonicus

LI Chengze, CHANG Yaqing, PANG Zhenguo, DING Jun, JI Nanjing

Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China

Abstract:

Environmental conditions, including ambient temperature, play important roles in survival, growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profi les in A. japonicus by suppression subtractive hybridization (SSH). Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profi les of these genes were investigated in different tissues (coelomocyte, respiratory tree, intestine, longitudinal muscle) after exposure to acute and mild temperature dropping treatments. The results show that (1) the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; (2) under the temperature dropping treatments, three types of transcriptional regulation patterns were observed: primary suppression followed by up-regulation at-2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and (3) gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature (-2℃).

Key words: Apostichopus japonicus|transcriptional regulation|low temperature stress|gene expression

Received: 2014-06-09 Revised: 2014-06-23

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Articles by CHANG Yaqing

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