Acute toxicity effects of perfluorooctane sulfonate on sperm vitality, kinematics and fertilization success in zebrafish -- Journal of Oceanology and Limnology,2017,35(4):723-728

	Cite this paper:
	XIA Jigang, NIU Cuijuan. Acute toxicity effects of perfluorooctane sulfonate on sperm vitality, kinematics and fertilization success in zebrafish[J]. Journal of Oceanology and Limnology, 2017, 35(4): 723-728

Acute toxicity effects of perfluorooctane sulfonate on sperm vitality, kinematics and fertilization success in zebrafish

XIA Jigang^1,2, NIU Cuijuan²

1 Laboratory of Evolutionary Physiology and Behavior, Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
2 Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China

Abstract:

Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertilization success in zebrafish (Danio rerio). Sperm were activated in aqueous media containing a range of PFOS concentrations (0, 0.09, 0.9 and 9 mg/L). Viabilities and kinematics of the sperm exposed to different PFOS treatments were assessed via computer-assisted sperm analysis (CASA) at 20, 40, 60, and 80 s after activation. PFOS exposure decreased the percentage of motile sperm, the curvilinear velocity (VCL), and the mean angular displacement (MAD) of spermatozoa, but showed no influence on the straightline velocity (VSL) or the angular path velocity (VAP). Furthermore, a significant decrease in fertilization success was observed in spermatozoa that were exposed to 0.9 mg/L PFOS or more. These findings indicate that PFOS pollution in natural aquatic environment may be a potential threaten to successful reproduction of fish.

Key words: perfluorooctane sulfonate (PFOS)|acute toxicity|sperm viability|sperm kinematics|fertilization success

Received: 2016-03-19 Revised: 2016-04-29

Tools

PDF (309 KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by XIA Jigang

Articles by NIU Cuijuan

References:
Alavi S M H, Cosson J. 2005. Sperm motility in fishes. I. Effects of temperature and pH:a review. Cell Biol. Int., 29(2):101-110.
Alavi S M H, Gela D, Rodina M, Linhart O. 2011. Roles of osmolality, calcium-potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm.Comp. Biochem. Phys. A, 160(2):166-174.
Alavi S M H, Rodina M, Viveiros A T M, Cosson J, Gela D, Boryshpolets S, Linhart O. 2009. Effects of osmolality on sperm morphology, motility and flagellar wave parameters in Northern pike (Esox lucius L.). Theriogenology, 72(1):32-43.
Ankley G T, Kuehl D W, Kahl M D, Jensen K M, Linnum A, Leino R L, Villeneuve D A. 2005. Reproductive and developmental toxicity and bioconcentration of perfluorooctanesulfonate in a partial life-cycle test with the fathead minnow (Pimephales promelas). Environ. Toxicol. Chem., 24(9):2 316-2 324.
Bobe J, Labbé C. 2010. Egg and sperm quality in fish. Gen.Comp. Endocrinol., 165(3):535-548.
Dorts J, Kestemont P, Marchand P A, D'Hollander W, Thézenas M L, Raes M, Silvestre F. 2011. Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS). Aquat. Toxicol., 103(1-2):1-8.
Du G Z, Hu J L, Huang H Y, Qin Y F, Han X M, Wu D, Song L, Xia Y K, Wang X R. 2013. Perfluorooctane sulfonate(PFOS) affects hormone receptor activity, steroidogenesis, and expression of endocrine-related genes in vitro and in vivo. Environ. Toxicol. Chem., 32(2):353-360.
Du Y B, Shi X J, Liu C S, Yu K, Zhou B S. 2009. Chronic effects of water-borne PFOS exposure on growth, survival and hepatotoxicity in zebrafish:a partial life-cycle test. Chemosphere, 74(5):723-729.
Giesy J P, Kannan K. 2001. Global distribution of perfluorooctane sulfonate in wildlife. Environ. Sci. Technol., 35(7):1 339-1 342.
Hagedorn M, McCarthy M, Carter V L, Meyers S A. 2012. Oxidative stress in zebrafish (Danio rerio) sperm. PLoS One, 7(6):e39 397.
Hagenaars A, Stinckens E, Vergauwen L, Bervoets L, Knapen D. 2014. PFOS affects posterior swim bladder chamber inflation and swimming performance of zebrafish larvae. Aquat. Toxicol., 157:225-235.
Han J, Fang Z Q. 2010. Estrogenic effects, reproductive impairment and developmental toxicity in ovoviparous swordtail fish (Xiphophorus helleri) exposed to perfluorooctane sulfonate (PFOS). Aquat. Toxicol., 99(2):281-290.
Hara Y, Strüssmann C A, Hashimoto S. 2007. Assessment of short-term exposure to nonylphenol in Japanese medaka using sperm velocity and frequency of motile sperm. Arch. Environ. Con. Tox., 53(3):406-410.
Hatef A, Alavi S M H, Golshan M, Linhart O. 2013. Toxicity of environmental contaminants to fish spermatozoa function in vitro-a review. Aquat. Toxicol., 140-141:134-144.
Hill A J, Teraoka H, Heideman W, Peterson R E. 2005. Zebrafish as a model vertebrate for investigating chemical toxicity. Toxicol. Sci., 86(1):6-19.
Hu W Y, Jones P D, DeCoen W, King L, Fraker P, Newsted J, Giesy J P. 2003. Alterations in cell membrane properties caused by perfluorinated compounds. Comp. Biochem. Phys. C, 135(1):77-88.
Huang H H, Huang C J, Wang L J, Ye X W, Bai C L, Simonich M T, Tanguay R L, Dong Q X. 2010. Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid(PFOS). Aquat. Toxicol., 98(2):139-147.
Inaba K. 2007. Molecular basis of sperm flagellar axonemes. Ann. NY. Acad. Sci., 1101:506-526.
Ingermann R L. 2008. Energy metabolism and respiration in fish spermatozoa. In:Alavi S M H, Cosson J, Coward K, Rafiee G eds. Fish Spermatology. Alpha Science Ltd, Oxford. p.241-266.
Jeon J, Lim H K, Kannan K, Kim S D. 2010. Effect of perfluorooctanesulfonate on osmoregulation in marine fish, Sebastesschlegeli, under different salinities. Chemosphere, 81(2):228-234.
Jing R Y, Huang C J, Bai C L, Tanguay R, Dong Q X. 2009. Optimization of activation, collection, dilution, and storage methods for zebrafish sperm. Aquaculture, 290(1-2):165-171.
Khan A T, Weis J S. 1987. Effects of methylmercury on sperm and egg viability of two populations of Killifish (Fundulus heteroclitus). Arch. Environ. Con. Tox., 16(4):499-505.
Kime D E, Ebrahimi M, Nysten K, Roelants I, Rurangwa E, Moore H D M, Ollevier F. 1996. Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish; application to the effects of heavy metals. Aquat. Toxicol., 36(3-4):223-237.
Kunacheva C, Fujii S, Tanaka S, Seneviratne S T M L D, Lien N P H, Nozoe M, Kimura K, Shivakoti B R, Harada H. 2012. Worldwide surveys of perfluorooctane sulfonate(PFOS) and perfluorooctanoic acid (PFOA) in water environment in recent years. Water Sci. Technol., 66(12):2 764-2 771.
Lahnsteiner F, Berger B, Grubinger F, Weismann T. 2005. The effect of 4-nonylphenol on semen quality, viability of gametes, fertilization success, and embryo and larvae survival in rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol., 71(4):297-306.
Lahnsteiner F, Patzner R A. 2008. Sperm morphology and ultrastructure in fish. In:Alavi S M H, Cosson J, Coward K, Rafiee G eds. Fish Spermatology. Alpha Science Ltd, Oxford. p.1-61.
Liu C S, Du Y B, Zhou B S. 2007. Evaluation of estrogenic activities and mechanism of action of perfluorinated chemicals determined by vitellogenin induction in primary cultured tilapia hepatocytes. Aquat. Toxicol., 85(4):267-277.
López-Doval S, Salgado R, Pereiro N, Moyano R, Lafuente A. 2014. Perfluorooctane sulfonate effects on the reproductive axis in adult male rats. Environ. Res., 134:158-168.
Morisawa M, Morisawa S. 1994. Acquisition and initiation of sperm motility. In:Gagnon C ed. Controls of Sperm Motility:Biological and Clinical Aspects. CRC Press, Boca Raton, FL. p.137-151.
Pereiro N, Moyano R, Blanco A, Lafuente A. 2014. Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic-pituitary-adrenal axis in adult male rats. Toxicol. Lett., 230(2):252-262.
Rosety M, Ordoñez F J, Rosety-Rodriguez M, Rosety J M, Rosety I. 2003. In vitro acute toxicity of anionic surfactant linear alkylbenzene sulphonate (LAS) on the motility of gilthead (Sparus aurata L.) sperm. Histol. Histopathol., 18(2):475-478.
Rurangwa E, Roelants I, Huyskens G, Ebrahimi M, Kime D E, Ollevier F. 1998. The minimum effective spermatozoa:egg ratio for artificial insemination and the effects of mercury on sperm motility and fertilization ability in Clarias gariepinus. J. Fish Biol., 53(2):402-413.
UNEP. 2009. Report of the conference of the parties of the Stockholm Convention on persistent organic pollutants on the work of its fourth meeting. UNEP/POPS/COP. 4/38.
Van Gossum H, Bots J, Snijkers T, Meyer J, Van Wassenbergh S, De Coen W, De Bruyn L. 2009. Behaviour of damselfly larvae (Enallagma cyathigerum) (Insecta, Odonata) after long-term exposure to PFOS. Environ. Pollut., 157(4):1 332-1 336.
Wang M Y, Chen J F, Lin K F, Chen Y H, Hu W, Tanguay R L, Huang C J, Dong Q X. 2011. Chronic zebrafish PFOS exposure alters sex ratio and maternal related effects in F1 offspring. Environ. Toxicol. Chem., 30(9):2 073-2 080.
Westerfield M. 1995. The Zebrafish Book:A Guide for the Laboratory Use of Zebrafish (Danio rerio). University of Oregon, Eugene, OR, USA. p.267-272.
Xia J G, Ma Y J, Guo W M, Huang L, Fu S J. 2015a. Temperature-dependent effects of PFOS on risk recognition and fast-start performance in juvenile Spinibarbus sinensis. Aquat. Biol., 24(2):101-108.
Xia J G, Nie L J, Mi X M, Wang W Z, Ma Y J, Cao Z D, Fu S J. 2015b. Behavior, metabolism and swimming physiology in juvenile Spinibarbus sinensis exposed to PFOS under different temperatures. Fish Physiol. Biochem., 41(5):1 293-1 304.
Zhang L L, Niu J F, Li Y, Wang Y J, Sun D. 2013. Evaluating the sub-lethal toxicity of PFOS and PFOA using rotifer Brachionus calyciflorus. Environ. Pollut., 180:34-40.
Zhang X, Chen L, Fei X C, Ma Y S, Gao H W. 2009. Binding of PFOS to serum albumin and DNA:insight into the molecular toxicity of perfluorochemicals. BMC Mol. Biol., 10:16.