Transcriptome profiles of embryos before and after cleavage in <i>Eriocheir sinensis</i>: identification of developmental genes at the earliest stages -- Journal of Oceanology and Limnology,2017,35(4):770-781

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	HUI Min, CUI Zhaoxia, LIU Yuan, SONG Chengwen. Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages[J]. Journal of Oceanology and Limnology, 2017, 35(4): 770-781

Transcriptome profiles of embryos before and after cleavage in Eriocheir sinensis: identification of developmental genes at the earliest stages

HUI Min¹, CUI Zhaoxia^1,2,3, LIU Yuan¹, SONG Chengwen^1,4

1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao 266071, China;
3 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
4 University of Chinese Academy of Sciences, Beijing 100049, China

Abstract:

In crab, embryogenesis is a complicated developmental program marked by a series of critical events. RNA-Sequencing technology offers developmental biologists a way to identify many more developmental genes than ever before. Here, we present a comprehensive analysis of the transcriptomes of Eriocheir sinensis oosperms (Os) and embryos at the 2-4 cell stage (Cs), which are separated by a cleavage event. A total of 18 923 unigenes were identified, and 403 genes matched with gene ontology (GO) terms related to developmental processes. In total, 432 differentially expressed genes (DEGs) were detected between the two stages. Nine DEGs were specifically expressed at only one stage. These DEGs may be relevant to stage-specific molecular events during development. A number of DEGs related to ‘hedgehog signaling pathway’, ‘Wnt signaling pathway’ ‘germplasm’, ‘nervous system’, ‘sensory perception’ and ‘segment polarity’ were identified as being up-regulated at the Cs stage. The results suggest that these embryonic developmental events begin before the early cleavage event in crabs, and that many of the genes expressed in the two transcriptomes might be maternal genes. Our study provides ample information for further research on the molecular mechanisms underlying crab development.

Key words: mitten crabs|embryo development|fertilized egg|cleavage|transcriptomics

Received: 2015-12-18 Revised: 2016-03-03

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