Improvement of <i>Nannochloropsis oceanica</i> growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling -- Journal of Oceanology and Limnology,2017,35(4):792-802

	Cite this paper:
	LIANG Sijie, GUO Li, LIN Genmei, ZHANG Zhongyi, DING Haiyan, WANG Yamei, YANG Guanpin. Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling[J]. Journal of Oceanology and Limnology, 2017, 35(4): 792-802

Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling

LIANG Sijie^1,2, GUO Li^1,2, LIN Genmei^1,2, ZHANG Zhongyi^1,2, DING Haiyan^1,2, WANG Yamei^1,2, YANG Guanpin^1,2,3

1 Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China;
2 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;
3 Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China

Abstract:

Nannochloropsis oceanica promises to be an industrial-level producer of polyunsaturated fatty acids. In this study, the fastest and slowest growing N. oceanica mutants were selected through N-methyl-N'-nitro-N-nitrosoguanidine mutation, and two mutant strains and the wild type (WT) subjected to transcriptome profiling. It was found that the OD₆₈₀ reads at stationary growth phase of both WT and its mutants were proportional to their cell density, thus indicating their division rate and growth speed during culture. This chemical mutation was effective for improving growth performance, and the fast strain divided faster by upregulating the expression of genes functioning in the cell cycle and downregulating genes involved in synthesis of amino acids, fatty acids, and sugars as well as the construction of ribosome and photosynthetic machinery. However, the relationship among the effected genes responsible for cell cycle, metabolism of fatty and amino acids, and construction of ribosome and photosynthetic machinery remained unclear. Further genetic studies are required for clarifying the genetic/metabolic networks underpinning the growth performance of N. oceanica. These findings demonstrated that this mutation strategy was effective for improving the growth performance of this species and explored a means of microalgal genetic improvement, particularly in species possessing a monoploid nucleus and asexual reproduction.

Key words: Nannochloropsis oceanica|mutation|cell cycle|transcriptome

Received: 2016-01-27 Revised: 2016-04-25

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Articles by LIANG Sijie

Articles by GUO Li

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