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Cite this paper: |
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WU Qingyang, WANG Shuqi, CHEN Xiaopeng, LI Ping. Reproductive toxicity assessment of benzo[a]pyrene in the marine polychaete Perinereis nuntia[J]. Journal of Oceanology and Limnology, 2017, 35(4): 867-873 |
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Reproductive toxicity assessment of benzo[a]pyrene in the marine polychaete Perinereis nuntia |
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WU Qingyang1, WANG Shuqi1, CHEN Xiaopeng2, LI Ping1 |
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1 Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China; 2 Administration of Ocean and Fisheries of Shantou, Shantou 515000, China |
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Abstract: |
Benzo[a]pyrene (B[a]P) is an increasingly present marine environmental pollutant, yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited. To test the reproductive toxicity of B[a]P on polychaetes, Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation, the sex ratio, number of eggs spawned, fertilization and hatching rated, as well as vitellogenin (VTG) mRNA expression levels were analyzed. A low concentration of B[a]P (2.5 μg/L) had no effects on the rate of sexual maturation, spawning, or fertilization but significantly increased the sex ratio (female:male) from 1.6±0.15:1 to 2.3±0.18:1, inhibited hatching rate by 27%, and significantly increased VTG mRNA expression level by 3.7-fold following a 60-day exposure, compared with those in the solvent controls. A higher concentration of B[a]P (25 μg/L) caused more serious effects; sexual maturation, fertilization success, and hatching decreased by 31%, 17% and 46%, respectively, and the sex ratio (female:male) and VTG mRNA gene expression level increased by 54% and 7.1-fold, respectively. These results demonstrate that sublethal concentrations of B[a]P negatively affect reproductive performance of the sandworm P. nuntia. |
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Key words:
Perinereis nuntia|benzo[a]pyrene|reproductive biology|vitellogenin
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Received: 2016-01-26 Revised: 2016-04-06 |
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