Cite this paper:
WANG Yiyan, SUN Hushan, WANG Yanjie, YAN Dongchun, WANG Lei. Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)[J]. Journal of Oceanology and Limnology, 2015, 33(2): 339-346

Cytochemical characterization of yolk granule acid phosphatase during early development of the oyster Crassostrea gigas (Thunberg)

WANG Yiyan, SUN Hushan, WANG Yanjie, YAN Dongchun, WANG Lei
College of Life Science, Ludong University, Yantai 264025, China
Abstract:
In this study, a cytochemical method and transmission electron microscopy was used to examine acid phosphatase activities of yolk granules throughout the early developmental stages of the Pacific oyster Crassostrea gigas. This study aimed to investigate the dynamic change of yolk granule acid phosphatase, and the mechanisms underlying its involvement in yolk degradation during the early developmental stages of molluscs. Three types of yolk granules (YGI, YGII, and YGⅢ) that differed in electron density and acid phosphatase reaction were identifi ed in early cleavage, morula, blastula, gastrula, trochophore, and veliger stages. The morphological heterogeneities of the yolk granules were related to acid phosphatase activity and degrees of yolk degradation, indicating the association of acid phosphatase with yolk degradation in embryos and larvae of molluscs. Fusion of yolk granules was observed during embryogenesis and larval development of C. gigas. The fusion of YGI (free of acid phosphatase reaction) with YGII (rich in acid phosphatase reaction) could be the way by which yolk degradation is triggered.
Key words:    cytochemistry|eggs|embryo|larvae|molluscs|Pacific oyster|yolk granules   
Received: 2013-12-29   Revised: 2014-02-17
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Articles by WANG Yiyan
Articles by SUN Hushan
Articles by WANG Yanjie
Articles by YAN Dongchun
Articles by WANG Lei
References:
Aladaileh S, Nair S V, Birch D, Raftos D A. 2007. Sydney rock oyster (Saccostrea glomerata) hemocytes: morphology and function. J. Invertebr. Pathol., 96 (1): 48-63.
Almendros A, Porcel D. 1992. Phosphatase activity in the hepatopancreas of Helix aspersa. Comp. Biochem. Physiol., 103 (A3): 455-460.
Ben C N, Li S G. 2001. Histochemistry. People's Medical Publishing House, Beijing, China. p.272-28. (in Chinese) Boucaud-Camou E, Roper C F E. 1995. Digestive enzymes in paralarval cephalopods. B. Mar. Sci., 57 (2): 313-327.
Cheng T C, Butler M S. 1979. Experimentally induced elevations in acid phosphatase activity in hemolymph of Biomphalaria glabrata (Mollusca). J. Invertebr. Pathol., 34 (2): 119-124.
Fagotto F. 1991. Yolk degradation in tick eggs: Ⅲ. Developmentally regulated acidification of the yolk spheres. Develop. Growth Differ., 33 (1): 57-66.
Fagotto F. 1995. Regulation of yolk degradation, or how to make sleepy lysosomes. J. Cell Sci., 108 (12): 3 645-3 647.
Fausto A, Gambellini G, Mazzini M, Cecchettini A, Masetti M, Giorgi F. 2001. Yolk granules are differentially acidifi ed during embryo development in the stick insect Carausius morosus. Cell Tissue Res., 305 (3): 433-443.
Fialho E, Nakamura A, Juliano L, Masuda H, Silva-Neto M A C. 2005. Cathepsin D-mediated yolk protein degradation is blocked by acid phosphatase inhibitors. Arch. Biochem. Biophys., 436 (2): 246-253.
Fialho E, Silveira A B, Masuda H, Silva-Neto M A C. 2002. Oocyte fertilization triggers acid phosphatase activity during Rhodnius prolixus embryogenesis. Insect Biochem. Molec., 32 (8): 871-880.
Komazaki S, Hiruma T. 1999. Degradation of yolk platelets in the early amphibian embryo is regulated by fusion with late endosomes. Dev. Growth Differ., 41 (2): 173-181.
Lemanski L F, Aldoroty R. 1977. Role of acid phosphatase in the breakdown of yolk platelets in developing amphibian embryos. J. Morph., 153 (3): 419-425.
Luna-González A, Maeda-Martínez A N, Ascencio-Valle F, Robles-Mungaray M. 2004. Ontogenetic variations of hydrolytic enzymes in the Pacific oyster Crassostrea gigas. Fish Shellfish Immun., 16 (3): 287-294.
Mallya S K, Partin J S, Valdizan M C, Lennarz W J. 1992. Proteolysis of the major yolk glycoproteins is regulated by acidification of the yolk platelets in sea urchin embryos. J. Cell Biol., 117 (6): 1 211-1 221.
Martin G G, Martin A, Tsai W, Hafner J C. 2011. Production of digestive enzymes along the gut of the giant keyhole limpet Megathura crenulata (Mollusca: Vetigastropoda). Comp. Biochem. Physiol., 160 (A3): 365-373.
Medina M, Vallejo C G. 1989. The maternal origin of acid hydrolases in Drosophila and their relation with yolk degradation. Dev. Growth Differ., 31 (3): 241-247.
Mohandas A, Cheng T C, Cheng J B. 1985. Mechanism of lysosomal enzyme release from Mercenaria mercenaria granulocytes: a scanning electron microscope study. J. Invertebr. Pathol., 46 (2): 189-197.
Oliveira D M P, Ramos I B, Reis F C G, Lima A P C A, Machado E A. 2008. Interplay between acid phosphatase and cysteine proteases in mediating vitellin degradation during early embryogenesis of Periplaneta americana. J. Insect Physiol., 54 (5): 883-891.
Oxford G S, Fish L J. 1979. Ultrastructural localization of esterase and acid phosphatase in digestive gland cells of fed and starved Cepaea nemoralis (L.) (Mollusca: Helicidae). Protoplasma, 101 (3): 181-196.
Pasteels J J. 1966. Multivesicular bodies of the egg of Barnea Candida studied with the electron microscope. J. Embryol. Exp. Morph., 16 (2): 301-310. (in French with English abstract)
Perona R, Bés J C, Vallejo C G. 1988. Degradation of yolk in the brine shrimp Artemia. Biochemical and morphological studies on the involvement of the lysosomal system. Biol. Cell., 63 (3): 361-366.
Perona R, Vallejo C G. 1985. Acid hydrolases during Artemia development: a role in yolk degradation. Comp. Biochem. Physiol., 81 (B4): 993-1 000.
Perona R, Vallejo C G. 1989. Mechanisms of yolk degradation in Artemia : a morphological study. Comp. Biochem. Physiol., 94 (A2): 231-242.
Pipe R K, Moore M N. 1985. The ultrastructural localization of lysosomal acid hydrolases in developing oocytes of the common marine mussel Mytilus edulis. Histochem. J., 17 (8): 939-949.
Ramos I B, Miranda K, Souza W, Machado E A. 2006. Calcium-regulated fusion of yolk granules during early embryogenesis of Periplaneta Americana. Mol. Reprod. Dev., 73 (10): 1 247-1 254.
Ribolla P E M, Daffre S, Bianchi A G D. 1993. Cathepsin B and acid phosphatase activities during Musca domestica embryogenesis. Insect Biochem. Mol. Biol., 23 (2): 217- 223.
Sun H S, Wang Y Y, Ling J G, Ran Z, Zeng P, Han Q. 2008. Acid and alkaline phosphatase activity in Mussel Mytilus edulis in early development stages. Oceanologia e t Limnologia Sinica, 39 (1): 42-47. (in Chinese with English abstract)
Volland J M, Gros O. 2012. Cytochemical investigation of the digestive gland of two strombidae species (Strombus gigas and Strombus pugili s) in relation to the nutrition. Microsc. Res. Techniq., 75 (10): 1 353-1 360.
Wang S, Wang Y, Ma J, Ding Y, Zhang S. 2011. Phosvitin plays a critical role in the immunity of zebrafish embryos via acting as a pattern recognition receptor and an antimicrobial effector. J. Biol. Chem., 286 (25): 22 653-22 664.
Yoshino T P, Cheng T C. 1976. Fine structural localization of acid phosphatase in granulocytes of the pelecypod Mercenaria mercenaria. Trans. Amer. Micros. Soc., 95 (2): 215-220.
Zhang S, Wang Z, Wang H. 2013. Maternal immunity in fi sh. Dev. Comp. Immunol., 39 (1-2): 72-78.
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