Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot <i>Scophthalmus maximus</i> based on response surface methodology -- Journal of Oceanology and Limnology,2015,33(1):28-36

	Cite this paper:
	HUANG Zhihui, MA Aijun, WANG Xin'an, LEI Jilin, LI Weiye, WANG Ting, YANG Zhi, QU Jiangbo. Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology[J]. Journal of Oceanology and Limnology, 2015, 33(1): 28-36

Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology

HUANG Zhihui^1,2, MA Aijun², WANG Xin'an², LEI Jilin², LI Weiye², WANG Ting², YANG Zhi³, QU Jiangbo³

1 College of Fisheries, Ocean University of China, Qingdao 266071, China;
2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China;
3 Yantai Tianyuan Aquatic Limited Corporation, Yantai 264003, China

Abstract:

Central Composite Design (CCD) and response surface methodology were used in the experiment to examine the combined effect of temperature (16-28℃) and salinity (18-42) on Hsp70 and IgM genes expression levels in turbot (Scophthalmus maximus) liver and kidney. The results showed that the coefficients of determination (R² =0.965 2 for liver Hsp70, 0.972 9 for kidney Hsp70, 0.921 for liver IgM and 0.962 1 for kidney IgM) and probability values (P <0.01) were significant for the regression model. The interactive effect between temperature and salinity on liver Hsp70, kidney Hsp70 and liver IgM were not significant (P >0.05), while the interactive effect between temperature and salinity on kidney IgM was significant (P <0.01). The model equation could be used in practice for forecasting Hsp70 and IgM genes expression levels in the liver and kidney of juvenile turbot via applying statistical optimization of the response of interest, at which the maximum liver Hsp70, kidney Hsp70, liver IgM and kidney IgM of 1.48, 1.49, 2.48, and 1.38, respectively, were reached. The present model may be valuable in assessing the feasibility of turbot farming at different geographic locations and, furthermore, could be a useful reference for scientists studying the immunity of turbot.

Key words: turbot (Scophthalmus maximus)|response surface methodology (RSM)|Central Composite Design (CCD)|Hsp70|immunoglobulin M (IgM)

Received: 2014-02-11 Revised: 2014-05-16

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