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ZHANG Chunhui, LIU Jianguo, ZHANG Litao. Cell cycles and proliferation patterns in Haematococcus pluvialis[J]. HaiyangYuHuZhao, 2017, 35(5): 1205-1211

Cell cycles and proliferation patterns in Haematococcus pluvialis

ZHANG Chunhui1,3, LIU Jianguo1,2, ZHANG Litao1,2
1 National & Local Joint Engineering Laboratory of Ecological Mariculture, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 National-Local Joint Engineering Research Center forHaematococcus pluvialisand Astaxanthin Products, Yunnan Alphy Biotech Co., Ltd., Chuxiong 675012, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China
Most studies on Haematococcus pluvialis have been focused on cell growth and astaxanthin accumulation; far less attention has been paid to cell cycles and proliferation patterns. The purpose of this study was to clarify cell cycles and proliferation patterns in H. pluvialis microscopically using a camera and video recorder system. The complicated life history of H. pluvialis can be divided into two stages:the motile stage and the non-motile stage. All the cells can be classified into forms as follows:motile cell, nonmotile cell, zoospore and aplanospore. The main cell proliferation, both in the motile phase and non-motile phase in H. pluvialis, is by asexual reproduction. Under normal growth conditions, a motile cell usually produces two, sometimes four, and exceptionally eight zoospores. Under unfavorable conditions, the motile cell loses its flagella and transforms into a non-motile cell, and the non-motile cell usually produces 2, 4 or 8 aplanospores, and occasionally 20-32 aplanospores, which further develop into non-motile cells. Under suitable conditions, the non-motile cell is also able to release zoospores. The larger non-motile cells produce more than 16 zoospores, and the smaller ones produce 4 or 8 zoospores. Vegetative reproduction is by direct cell division in the motile phase and by occasional cell budding in the non-motile phase. There is, as yet, no convincing direct evidence for sexual reproduction.
Key words:    size frequency|length-weight|sex ratio|allometric growth|Clupisoma garua   
Received: 2016-03-31   Revised: 2016-05-06
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